A5067840886- Preterm Birth and Chorioamnionitis
- Pregnancy-related medical research
- Reproductive System and Pregnancy
- Urological Disorders and Treatments
- Pregnancy and preeclampsia studies
- Neonatal Respiratory Health Research
- Birth, Development, and Health
- Pediatric Urology and Nephrology Studies
- Advanced Proteomics Techniques and Applications
- Philosophy and History of Science
- Urinary and Genital Oncology Studies
- Gestational Diabetes Research and Management
- Bladder and Urothelial Cancer Treatments
- Ecosystem dynamics and resilience
- Gastrointestinal disorders and treatments
- Evolutionary Game Theory and Cooperation
- Maternal and Perinatal Health Interventions
- Urologic and reproductive health conditions
- Evolution and Science Education
- Apelin-related biomedical research
- Extracellular vesicles in disease
- Environmental Philosophy and Ethics
- Species Distribution and Climate Change
- Teratomas and Epidermoid Cysts
- Tumors and Oncological Cases
Uppsala University Hospital
2006-2022
Western University
2013-2021
Uppsala University
2014-2020
The University of Melbourne
1991-2018
Mercy Hospital for Women
2010-2018
University of Bristol
2016
Baker Heart and Diabetes Institute
2010-2012
The University of Queensland
2010-2012
Bucknell University
2005-2007
Royal Women's Hospital
2003-2005
Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these in the search for potential diagnostic/prognostic biomarkers serum patients has been limited by presence highly abundant albumin immunoglobulins that constitute approximately 60-97% total proteins. purpose study was evaluate whether treatment human with Affi-Gel Blue alone or combination Protein A (Aurum protein mini kit, Bio-Rad) before two-dimensional gel electrophoresis...
A tenet of contemporary obstetrics is that a significant proportion preterm births involve bacterial infection. Bacterial endotoxin induces pro-inflammatory cytokines, prostaglandins and proteases via the pathway nuclear factor-κB (NF-κB), which plays key role in initiating uterine contractions rupture foetal membranes. In non-gestational tissues, phytophenols curcumin, naringenin apigenin exert anti-inflammatory properties inhibition NF-κB. The aim this study was to determine whether these...
EACH CHAPTER ENDS WITH SUGGESTIONS FOR FURTHER READING. 1: SCIENCE AND PHILOSOPHY 1.0 Scientific disputes and philosophical questions 1.1 Modern science: A brief history 1.2 Images of Science 2: THE ANALYTIC PROJECT 2.0 Demarcating 2.1 Confirmation 2.2 Theories 2.3 Explanation 2.4 Failures-and Successes 3: VIEW FROM SCIENCES 3.0 The sciences on their own terms 3.1 ideal unified science 3.2 ineradicability causation 3.3 Against the supernatural 3.4 Making sense ourselves 3.5 Naturalizing...
Epithelial ovarian cancer is the fourth leading cause of death among women. Due to asymptomatic nature and poor survival characteristic disease, screening for specific biomarkers a major health priority. Differentially expressed proteins in serum patients have potential be used as cancer-specific biomarkers. In this study, proteomic methods were screen 24 samples from women with high-grade compared control group 11 healthy Affigel-Blue treated processed either by linear (pH 4–7) or narrow...
Human sirtuin (SIRT) 1 and SIRT2, which possess nicotinamide adenosine dinucleotide (NAD+)-dependent deacetylase activity, exhibit anti-inflammatory actions. However, there are no data available on SIRT1 SIRT2 expression regulation in human intrauterine tissues. Thus, the aim of this study was to characterize localization 1) placenta fetal membranes before after term spontaneous labor onset, 2) prelabor from supracervical site (SCS) a distal (DS), 3) response proinflammatory stimuli....
The primary hypothesis to be tested in this study was that the diagnostic performance (as assessed by area under receiver operator characteristic curve, AUC) of a multianalyte panel correctly identify women with ovarian cancer significantly greater than for CA-125 alone.A retrospective, case-control (phase II biomarker trial) conducted involved 362 plasma samples obtained from (n = 150) and healthy controls 212). A multivariate classification model developed incorporated five biomarkers...
Screening for specific biomarkers of early-stage detection ovarian cancer is a major health priority due to the asymptomatic nature and poor survival characteristic disease. We utilised two-dimensional gel electrophoresis (2DE) identify differentially expressed proteins in serum patients that may be useful as this In study, 38 at different pathological grades (grade 1 (n=6), grade 2 (n=8) 3 (n=24)) were compared control group eight healthy women. Serum samples treated with mixture...
In order to determine the outcome and associated chromosomal structural anomalies in fetuses diagnosed utero as having a congenital diaphragmatic hernia, we reviewed 48 consecutive cases referred our regional Fetal Diagnostic Unit between 1988 1995. All babies were delivered units with appropriate neonatal resuscitation facilities. Thirteen [34 per cent of those tested, confidence interval (CI) 19-49 cent] had karyotypic abnormalities. Three trisomies but other nine more complex...
Objective The aim of this study was to determine (i) the effect maternal obesity and gestational diabetes mellitus (GDM) on circulating levels omentin-1 in cord plasma, (ii) gene expression release from human placenta adipose tissue. pregnancy also determined. Design Omentin-1 were measured plasma obese non-obese normal glucose tolerant women (NGT; n = 44) with GDM (n 39) at time term elective Caesarean section. Placenta tissue 22 NGT collected 13 11 28 weeks gestation 7 postpartum. Results...
Problem Toll‐like receptors ( TLR s) 2 and 5 induce inflammation via the adapter proteins myeloid differentiation factor 88 M y D 88) TNFR ‐associated 6 TRAF 6) transcription nuclear factor‐kappa B NF ‐κ ). The aims of this study were to determine effects ligand flagellin FSL ‐1 on pro‐inflammatory pro‐labour mediators in human fetal membranes myometrium, establish whether their actions are dependent 88, . Method Study Tissue explants performed effect myometrium. si RNA knockdown was primary...
Preterm birth is a major determinant of neonatal mortality and morbidity, affecting approximately one-third preterm births as result prelabor rupturing membranes. Infection inflammation have strong causal links to delivery, resulting in the activation nuclear factor-kappaB (NFKB) its downstream targets. Human sirtuin (SIRT) 6, which has ADP-ribosyl transferase deacetylase activity, exhibits anti-inflammatory actions. The aims this study were determine effect 1) human labor on SIRT6...
Our aim was to study the protein expression profiles of placenta obtained from lean and obese pregnant women with normal glucose tolerance at time term Caesarean section. We used two-dimensional difference gel electrophoresis (2D-DIGE), utilising narrow-range immobilised pH gradient strips that encompassed broad range 4-5 5-6, followed by MALDI-TOF mass spectrometry selected spots. Western blot quantitative RT-PCR (qRT-PCR) analyses were performed validate representative findings 2D-DIGE...
Gestational diabetes mellitus (GDM) is a significant risk factor for the type 2 epidemic in many populations. Maternal adipose tissue plays central role pathophysiology of GDM. Thus, aim this study was to determine effect GDM on proteome tissue. Omental obtained at time term Caesarean section from women with normal glucose tolerance (NGT) or 2D-difference gel electrophoresis (DIGE), followed by mass spectrometry, used identify protein spots ( n =6 patients per group). Western blotting...
Gestational diabetes mellitus (GDM), which complicates up to 20% of all pregnancies, is associated with low-grade maternal inflammation and peripheral insulin resistance. Sterile infection are key mediators this Resveratrol, a stilbene-type phytophenol, has been implicated exert beneficial properties including potent anti-inflammatory antidiabetic effects in non-pregnant humans experimental animal models GDM. However, studies showing the resveratrol on resistance GDM human tissues have...
Abstract Functional diversity holds the promise of understanding ecosystems in ways unattainable by taxonomic studies. Underlying this is intuition that investigating what organisms actually do (i.e., their functional traits) within will generate more reliable insights into these behave, compared to considering only species diversity. But also rests on several conceptual and methodological epistemic) assumptions cut across various theories domains ecology. These should be clearly addressed,...
The peroxisome proliferator-activated receptors (PPARs) belong to a subclass of nuclear hormone receptor that executes important cellular transcriptional functions. Previous studies have demonstrated the expression PPARγ in several tumours including colon, breast, bladder, prostate, lung and stomach. This study demonstrates relative normal ovaries different pathological grades ovarian serous, mucinous, endometrioid, clear cell mixed subtypes. A total 56 specimens 10 normal, eight benign,...
Preterm birth is the leading factor causing neonatal mortality and morbidity. Inflammation plays a central role in stimulating uterine contractility, which responsible for approximately one-third of all preterm births. Recent studies have shown that transcription Forkhead box O3 (FOXO3) regulates inflammation nongestational tissues such as adipocytes hepatocytes. Thus, this study, we sought to determine effect 1) human term labor on myometrial FOXO3 expression 2) inhibition overexpression...
Preterm birth is the largest single cause of neonatal death and morbidity. By activating cytokine- Toll-like receptor (TLR)-signaling pathways, infection and/or inflammation are strongly associated with preterm delivery. Interferon regulatory factor-1 (IRF1) an important regulator inflammatory response. The aims this study were to establish effect 1) labor on IRF1 expression in human fetal membranes myometrium, 2) prolabor mediators activity, 3) small interfering RNA mediators. was higher...
Infection-induced preterm birth is the largest cause of infant death and neurological disabilities in survivors. Silibinin, from milk thistle, exerts potent anti-inflammatory activities non-gestational tissues. The aims this study were to determine effect silibinin on pro-inflammatory mediators (i) human fetal membranes myometrium treated with bacterial endotoxin lipopolysaccharide (LPS) or cytokine IL-1β, (ii) active infection. infection induced inflammation brain injury pregnant mice was...