A5005942893- Preterm Birth and Chorioamnionitis
- Reproductive System and Pregnancy
- Pregnancy-related medical research
- Pregnancy and preeclampsia studies
- Influenza Virus Research Studies
- Gestational Diabetes Research and Management
- Birth, Development, and Health
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Respiratory viral infections research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- Atherosclerosis and Cardiovascular Diseases
- Apelin-related biomedical research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Immune cells in cancer
- COVID-19 Impact on Reproduction
- Cardiovascular Disease and Adiposity
- Cardiovascular Issues in Pregnancy
- Neuroinflammation and Neurodegeneration Mechanisms
- Infant Nutrition and Health
- Hepatitis Viruses Studies and Epidemiology
- Neuroendocrine Tumor Research Advances
- Respiratory Support and Mechanisms
RMIT University
2019-2025
National Cancer Centre Singapore
2024-2025
Victoria University
2023
Mercy Hospital for Women
2013-2018
The University of Melbourne
2011-2018
Royal Women's Hospital
2016
Mount Sinai Hospital
2012
Institute of Cancer Research
1997
Aims: Reactive oxygen species (ROS) are highly reactive molecules generated in different subcellular sites or compartments, including endosomes via the NOX2-containing nicotinamide adenine dinucleotide phosphate oxidase during an immune response and mitochondria cellular respiration. However, while endosomal NOX2 promotes innate inflammation to influenza A virus (IAV) infection, role of mitochondrial ROS (mtROS) has not been comprehensively investigated context viral infections vivo....
Objective To identify cervicovaginal fluid ( CVF ) biomarkers predictive of spontaneous preterm birth in women with symptoms labour. Design Retrospective cohort study. Setting Melbourne, A ustralia. Population Women a singleton pregnancy admitted to the Emergency Department between 22 and 36 weeks gestation presenting Methods Two‐dimensional electrophoresis was used analyse proteome. Validation putative performed using enzyme‐linked immunosorbent assay ELISA an independent cohort. Optimal...
Significance Influenza infection during pregnancy is associated with increased maternal and perinatal complications. Here, we show that, pregnancy, influenza leads to viral dissemination into the aorta, resulting in a peripheral “vascular storm” characterized by enhanced inflammatory mediators; influx of Ly6C monocytes, neutrophils, T cells; impaired vascular function. The ensuing storm induced hypoxia placenta fetal brain caused an increase circulating cell free DNA soluble Flt1 release. We...
Abstract Toll-like receptor 7 (TLR7) is a pattern recognition that recognizes viral RNA following endocytosis of the virus and initiates powerful immune response characterized by Type I IFN production pro-inflammatory cytokine production. Despite this response, causes very significant pathology, which may be inflammation-dependent. In present study, we examined effect intranasal delivery TLR7 agonist, imiquimod or its topical formulation Aldara, on inflammation pathogenesis caused IAV...
Macrophages undergo a metabolic switch from oxidative phosphorylation to glycolysis when exposed gram-negative bacterial lipopolysaccharide (LPS), which modulates antibacterial host defence mechanisms. Here, we show that LPS treatment of macrophages increased the classical burst response via NADPH oxidase (NOX) 2 enzyme, was blocked by 2-deoxyglucose (2-DG) inhibition glycolysis. The pentose phosphate pathway with 6-aminonicotinamide (6-AN) also suppressed LPS-induced increase in NOX2...
ABSTRACT Specific protein receptors that mediate internalization and entry of influenza A virus (IAV) have not been identified for any cell type. Sialic acid (SIA), the primary attachment factor IAV hemagglutinin, is expressed by numerous surface glycoproteins glycolipids, confounding efforts to identify specific involved in infection. Lec1 Chinese hamster ovary (CHO) epithelial cells express SIA bind yet are largely resistant Here, we demonstrate expression murine macrophage galactose-type...
Gestational diabetes mellitus (GDM), which complicates up to 20% of all pregnancies, is associated with low-grade maternal inflammation and peripheral insulin resistance. Sterile infection are key mediators this Resveratrol, a stilbene-type phytophenol, has been implicated exert beneficial properties including potent anti-inflammatory antidiabetic effects in non-pregnant humans experimental animal models GDM. However, studies showing the resveratrol on resistance GDM human tissues have...
Maternal obesity and gestational diabetes mellitus (GDM) are two increasingly common important obstetric complications that associated with severe long-term health risks to mothers babies. IL-1β, which is increased in obese GDM pregnancies, plays an role the pathophysiology of these pregnancy complications. In non-pregnant tissues, endoplasmic (ER) stress can induce IL-1β via inflammasome activation. The aim this study was determine whether ER omental adipose tissue women GDM, if also...
328 Background: The prognosis of PLGC patients (pts) is poor with standard systemic therapy. pts who undergo CRS after BD chemotherapy (chemo) demonstrate prolonged survival. We retrospectively analysed the outcomes underwent chemo followed by and HIPEC in a single institution, stratifying peritoneal carcinomatosis index (PCI) regimen. Methods: received between January 2016 to September 2023 were enrolled consented for data collection. was either 3 weekly paclitaxel (PTX) (50mg/m2), S-1...
TLR7 plays a key role in recognizing viral RNA to initiate an immune response. Sex-based differences the severity of RSV respiratory infections have been noted, and this may be related higher expression X-linked toll-like receptor 7 (TLR7) female cells. Indeed, has shown influence sex responses other viruses; however, its infection remains underexplored. We infected adult C57Bl/6 or knockout mice with compared specific lung between different sexes. Gene analysis revealed that had elevated...
Vesicular stomatitis virus, human immunodeficiency virus type 2, and foamy which were produced by cell lines expressing galactosyl(alpha1-3)galactosyl (alphaGal) sugars, found to be less stable in serum than those from alphaGal-negative cells, indicating that galactosyl(alpha1-3)galactosylation sensitizes these viruses as well mammalian C oncoviruses (Rother et al., J. Exp. Med. 182:1345-1355, 1995; Takeuchi Nature (London) 379:85-88, 1996) complement killing via natural anti-alphaGal...
Increasing evidence indicates that endoplasmic reticulum (ER) stress is involved in various diseases. In nongestational tissues, several markers of the unfolded protein response (UPR) have been shown to regulate inflammatory response. Thus, aim this study was determine effect human labor on ER fetal membranes and myometrium. addition, inhibition expression secretion proinflammatory prolabor mediators also assessed. The stress, GRP78, IRE1, spliced XBP1 (XBP1s), were significantly increased...
Preterm birth is the largest single cause of neonatal death and morbidity. By activating cytokine- Toll-like receptor (TLR)-signaling pathways, infection and/or inflammation are strongly associated with preterm delivery. Interferon regulatory factor-1 (IRF1) an important regulator inflammatory response. The aims this study were to establish effect 1) labor on IRF1 expression in human fetal membranes myometrium, 2) prolabor mediators activity, 3) small interfering RNA mediators. was higher...
Background and Objective Neurocognitive dysfunction is present in up to ∼61% of people with chronic obstructive pulmonary disease (COPD), symptoms including learning memory deficiencies, negatively impacting the quality life these individuals. As mechanisms responsible for neurocognitive deficits COPD remain unknown, we explored whether cigarette smoke (CS) exposure causes mice this associated neuroinflammation an altered neuropathology. Methods Male BALB/c were exposed room air (sham) or CS...
Influenza A virus (IAV) infection in pregnancy resembles a preeclamptic phenotype characterised by vascular dysfunction and foetal growth retardation. Given that low dose aspirin (ASA) is safe used to prevent preeclampsia, we investigated whether ASA or NO-conjugated aspirin, NCX4016, resolve inflammation function improve offspring outcomes following IAV pregnant mice. Pregnant mice were intranasally infected with mouse adapted strain (Hkx31; 10 4 plaque forming units) received daily...
Respiratory syncytial virus (RSV) commonly infects the upper respiratory tract (URT) of humans, manifesting with mild cold or flu-like symptoms. However, in infants and elderly, severe disease lower (LRT) often occurs can develop into chronic airway disease. A better understanding how an acute RSV infection transitions to a LRT inflammatory is critically important improve patient care long-term health outcomes. To model phases disease, we infected wild-type C57BL/6 toll-like receptor 7...
The ability to recognise women who are at-risk of preterm labour (PTL) is often difficult. Over 50% identified with factors associated an increased risk birth will ultimately deliver at term. cervicovaginal fluid (CVF) comprises a range proteins secreted by gestational tissues, making it ideal candidate for the screening differentially expressed PTL. CVF samples were collected from asymptomatic women. Two-dimensional gel electrophoresis techniques used examine proteome spontaneously...
Vitamin D binding protein (VDBP) has previously been identified in the amniotic fluid and cervicovaginal (CVF) of pregnant women. The biological functions VDBP include acting as a carrier for vitamin metabolites, clearance actin that is released during tissue injury augmentation pro-inflammatory response. This longitudinal observational study was conducted on 221 healthy women who spontaneously laboured delivered either at term or preterm. Serial CVF samples were collected measured by ELISA....