A5027244580- Influenza Virus Research Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Birth, Development, and Health
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Pregnancy and preeclampsia studies
- Cardiomyopathy and Myosin Studies
- Cardiovascular Function and Risk Factors
- Inflammasome and immune disorders
- Cardiovascular Disease and Adiposity
- Eicosanoids and Hypertension Pharmacology
- Sodium Intake and Health
- Respiratory viral infections research
- Atherosclerosis and Cardiovascular Diseases
- Medical and Biological Ozone Research
- Immune cells in cancer
- Neuroendocrine regulation and behavior
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Cardiac Fibrosis and Remodeling
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Hormonal Regulation and Hypertension
- Endoplasmic Reticulum Stress and Disease
- Vitamin C and Antioxidants Research
- Antioxidant Activity and Oxidative Stress
- Tryptophan and brain disorders
RMIT University
2019-2024
Hudson Institute of Medical Research
2022-2023
Monash University
2022-2023
Baker Heart and Diabetes Institute
2017-2021
MIT University
2021
Aims: Reactive oxygen species (ROS) are highly reactive molecules generated in different subcellular sites or compartments, including endosomes via the NOX2-containing nicotinamide adenine dinucleotide phosphate oxidase during an immune response and mitochondria cellular respiration. However, while endosomal NOX2 promotes innate inflammation to influenza A virus (IAV) infection, role of mitochondrial ROS (mtROS) has not been comprehensively investigated context viral infections vivo....
Significance Influenza infection during pregnancy is associated with increased maternal and perinatal complications. Here, we show that, pregnancy, influenza leads to viral dissemination into the aorta, resulting in a peripheral “vascular storm” characterized by enhanced inflammatory mediators; influx of Ly6C monocytes, neutrophils, T cells; impaired vascular function. The ensuing storm induced hypoxia placenta fetal brain caused an increase circulating cell free DNA soluble Flt1 release. We...
Macrophages undergo a metabolic switch from oxidative phosphorylation to glycolysis when exposed gram-negative bacterial lipopolysaccharide (LPS), which modulates antibacterial host defence mechanisms. Here, we show that LPS treatment of macrophages increased the classical burst response via NADPH oxidase (NOX) 2 enzyme, was blocked by 2-deoxyglucose (2-DG) inhibition glycolysis. The pentose phosphate pathway with 6-aminonicotinamide (6-AN) also suppressed LPS-induced increase in NOX2...
Influenza A virus (IAV) infection in pregnancy resembles a preeclamptic phenotype characterised by vascular dysfunction and foetal growth retardation. Given that low dose aspirin (ASA) is safe used to prevent preeclampsia, we investigated whether ASA or NO-conjugated aspirin, NCX4016, resolve inflammation function improve offspring outcomes following IAV pregnant mice. Pregnant mice were intranasally infected with mouse adapted strain (Hkx31; 10 4 plaque forming units) received daily...
Respiratory syncytial virus (RSV) commonly infects the upper respiratory tract (URT) of humans, manifesting with mild cold or flu-like symptoms. However, in infants and elderly, severe disease lower (LRT) often occurs can develop into chronic airway disease. A better understanding how an acute RSV infection transitions to a LRT inflammatory is critically important improve patient care long-term health outcomes. To model phases disease, we infected wild-type C57BL/6 toll-like receptor 7...
Diabetes is a major contributor to the increasing burden of heart failure prevalence globally, at least in part due disease process termed diabetic cardiomyopathy. Diabetic cardiomyopathy characterised by cardiac structural changes that are caused chronic exposure milieu. These cause left ventricular (LV) wall stiffness and development LV dysfunction. In current study, we investigated therapeutic potential cardiac-targeted bone morphogenetic protein 7 (BMP7) gene therapy, administered once...
Influenza A virus (IAV) infection during pregnancy initiates significant aortic endothelial and vascular smooth muscle dysfunction, with inflammation T cell activation, but the details of mechanism are yet to be clearly defined. Here we demonstrate that IAV disseminates preferentially into perivascular adipose tissue (PVAT) aorta in mice. mRNA levels PVAT increased at 1–3 days post (d.p.i) being ~4–8 fold higher compared vessel wall. also Ly6C low patrolling monocytes high pro-inflammatory...
Abstract Objectives Novel host‐targeted therapeutics could treat severe influenza A virus (IAV) infections, with reduced risk of drug resistance. LAT8881 is a synthetic form the naturally occurring C‐terminal fragment human growth hormone. Acting independently hormone receptor, it can reduce inflammation‐induced damage and promote tissue repair in an animal model osteoarthritis. has been assessed clinical trials for treatment obesity neuropathy excellent safety profile. We investigated...
Influenza A virus (IAV) infection during pregnancy can increase the risk for neurodevelopmental disorders in offspring, however, underlying neurobiological mechanisms are largely unknown. To recapitulate viral infection, preclinical studies have traditionally focused on using synthetic mimetics, rather than live IAV, to examine consequences of maternal immune activation (MIA)-dependent processes offspring. In contrast, few used IAV assess effects global gene expression, and none date...
There is an urgent need to develop effective therapeutic strategies including immunomodulators combat influenza A virus (IAV) infection. Influenza viruses increase ROS production, which suppress anti-viral responses and contribute pathological inflammation morbidity. Two major cellular sites of production are endosomes via the NOX2-oxidase enzyme electron transport chain in mitochondria. Here we examined effect administration Cgp91ds-TAT, endosome-targeted NOX2 oxidase inhibitor, combination...
People with chronic obstructive pulmonary disease (COPD) are susceptible to respiratory infections which exacerbate and/or cardiovascular complications, increasing their likelihood of death. The mechanisms driving these complications remain unknown but increased oxidative stress has been implicated. Here we investigated whether influenza A virus (IAV) infection, following cigarette smoke (CS) exposure, worsens vascular function and if so, the antioxidant ebselen alleviates this dysfunction....
T-cell accumulation within the aorta promotes endothelial dysfunction and genesis of cardiovascular disease, including hypertension atherosclerosis. Viral infection during pregnancy is also known to mediate marked acute dysfunction, but it not clear whether T cells are recruited persists postpartum. Here, we demonstrate that influenza A virus (IAV) in a murine model resulted aorta, which persisted for up 60 days postinfection was associated with higher levels IFN-γ mRNA expression tissue. In...
Background and Purpose: People with COPD are susceptible to respiratory infections which exacerbate pulmonary and/or cardiovascular complications, increasing their likelihood of death. The mechanisms driving these complications remain unknown but increased oxidative stress has been implicated. Here we investigated whether influenza A virus (IAV) infection, following chronic cigarette smoke (CS) exposure, worsens vascular function if so, the antioxidant ebselen alleviates this dysfunction....