A5081054983- Blood properties and coagulation
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Erythrocyte Function and Pathophysiology
- Ovarian function and disorders
- Blood Coagulation and Thrombosis Mechanisms
- Gynecological conditions and treatments
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Estrogen and related hormone effects
- Protease and Inhibitor Mechanisms
- Acute Myocardial Infarction Research
- Protein purification and stability
- Proteoglycans and glycosaminoglycans research
- Blood groups and transfusion
- Reproductive Biology and Fertility
- Potato Plant Research
- Advanced Biosensing Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Preterm Birth and Chorioamnionitis
- Connective tissue disorders research
- Retinoids in leukemia and cellular processes
- Health and Medical Research Impacts
- Endoplasmic Reticulum Stress and Disease
- Hemostasis and retained surgical items
Hudson Institute of Medical Research
2014-2023
Monash University
2014-2023
Monash Institute of Medical Research
2012-2014
Monash Medical Centre
2012
Xilinx (United States)
2010
National Institute for Biological Standards and Control
1995-1999
Nottingham Trent University
1998
Royal Veterinary College
1992-1996
University of London
1992-1996
Potters Bar Community Hospital
1995
The primary hypothesis to be tested in this study was that the diagnostic performance (as assessed by area under receiver operator characteristic curve, AUC) of a multianalyte panel correctly identify women with ovarian cancer significantly greater than for CA-125 alone.A retrospective, case-control (phase II biomarker trial) conducted involved 362 plasma samples obtained from (n = 150) and healthy controls 212). A multivariate classification model developed incorporated five biomarkers...
The aims of this study were: to characterise and compare plasma concentrations midkine (MDK) in normal healthy women with observed ovarian cancer; establish the performance MDK that anterior gradient 2 protein (AGR2) CA125 development multi-analyte classification algorithms for cancer. Median immunoreactive MDK, AGR2 were significantly greater case cohort (909 pg/ml, 765 pg/ml 502 U/ml, respectively n = 46) than control (383 188 13 61) (p < 0.001). area under receiver operator characteristic...
Ovarian cancer is often asymptomatic and diagnosed at an advanced stage with poor survival rates, thus there urgent need to develop biomarkers for earlier detection of ovarian cancer. In the present study, we demonstrate first time that previously reported metastasis-inducing protein AGR2 (anterior gradient 2) can be detected in blood patients. Using a newly developed ELISA, show significantly increased concentrations plasma from patients relative normal controls. Plasma were highest stages...
The in vivo formation of fibrin and its subsequent secondary fibrinolytic digestion yields a variety crosslinked degradation products (XL/FnDP). One these is known as D-dimer ELISA-type commercial kits using monoclonal antibodies to have been developed. We investigated the possibility establishing standard such that various might indicate same levels samples. concluded because it seems each individual antibody targets unique distinct epitope FnDP fraction notion introducing which will...
The regenerative, proliferative phase of a woman's menstrual cycle is critical period which lays the foundation for subsequent, receptive secretory phase. Although endometrial glands and their secretions are essential embryo implantation survival, phase, when these form, has been rarely examined. We hypothesized that alterations in secreted proteome endometrium idiopathic infertile women would reflect disturbance regeneration. Our aim was to compare proteomic profile uterine fluid from...
A healthy pregnancy requires successful blastocyst implantation into an adequately prepared or 'receptive' endometrium. Decidualization of uterine endometrial stromal fibroblast cells (hESF) is critical for the establishment a pregnancy. microRNAs (miRs) are regulators cellular function that can be released by donor cell to influence physiological state recipient cells. We aimed determine how decidualization affects hESF miR release and investigated one regulated miR, miR-19b-3p, previously...
The endometrium undergoes substantial morphological and functional changes to become receptive embryo implantation enable establishment of a successful pregnancy. Reduced Delta-like ligand 1 (DLL1, Notch ligand) in the is associated with infertility. DLL1 can be cleaved by 'a disintegrin metalloprotease' (ADAM) proteases produce soluble that may act inhibit signalling. We used an enzyme-linked immunosorbent assay quantify uterine lavages from fertile infertile women secretory phase menstrual...
Summary Four monoclonal antibodies have previously been raised in our laboratory for possible use thrombus imaging and the targeting of thrombolytic agents. These were to various human fibrin-related immunogens each antibody had selected its specificity towards fibrin not fibrinogen. To study further these animal circulation models both vivo vitro, their selectivity as opposed other fibrins was examined. In this dissociation constants with six species (human, baboon, pig, dog, sheep, rabbit)...
The current International Standard (IS) for alpha-thrombin was established in 1991. It related unitage with the 1st IS Thrombin 1975 and contains 100 IU of thrombin activity. National Institute Health (NIH) standard (Lot J) is common use calibration commercial reagents this study reports on a comparison units defined by these 2 standards. This has indicated that one "NIH" unit equivalent to 1.1 1.3 thrombin, depending influence PEG assay. A ratio figure 1.15 recommended following analysis...
There is frequent use of human and animal proteins as stabilizers during lyophilization a variety biological substances with view to long term stable storage. This report describes the comparative excellent stabilizing effect porcine collagen peptide fraction (CPF) subsequent storage three commonly used substances, alkaline phosphatase, tissue plasminogen activator thrombin. The CPF was heated 150 degrees C for one hour before use. shown have some advantage lyophilized over serum albumin....
Summary Potato tubers of the cultivar Pentland Dell were investigated for ultrastructural changes following impact. Studies carried out on after 12 and 24 wk storage using a falling bolt delivering 0.7 J energy. Ultra‐thin sections examined by light transmission electron microscopy. Tubers stored exhibited no visual bruise formation, whereas visible bruising resulted impact wk. An sequence events during was established as (1) collapse intracellular compartmentation, (2) increased ribosomal...
Since the finding that plasminogen activator inhibitor-1 (PAI-1) may influence initiation and progression of acute myocardial infarction, assay PAI-1 in plasma using a variety commercial kits has become commonplace. The need for standard to define activity prompted an international collaborative study (ICS) calibrate functional potency lyophilised preparation (92/654). inhibits 2 major activators, tissue-type (t-PA) urinary-type (u-PA) equimolar manner it was important establish inhibitor...