A5089425163- S100 Proteins and Annexins
- Protease and Inhibitor Mechanisms
- Bone and Dental Protein Studies
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Thyroid Cancer Diagnosis and Treatment
- Fibroblast Growth Factor Research
- Cancer-related Molecular Pathways
- Wnt/β-catenin signaling in development and cancer
- RNA modifications and cancer
- Cell Adhesion Molecules Research
- Animal Genetics and Reproduction
- Cancer-related gene regulation
- Proteoglycans and glycosaminoglycans research
- Biomarkers in Disease Mechanisms
- Heat shock proteins research
- Peptidase Inhibition and Analysis
- Cellular Mechanics and Interactions
- Cancer, Hypoxia, and Metabolism
- ATP Synthase and ATPases Research
- Computational Drug Discovery Methods
- Neonatal Respiratory Health Research
- Endoplasmic Reticulum Stress and Disease
- HER2/EGFR in Cancer Research
- Connexins and lens biology
University of Liverpool
2012-2023
Cancer and Polio Research Fund
1999-2011
Molecular Oncology (United States)
2010
Mater Health Services
2010
Royal Liverpool University Hospital
2006
Christchurch Hospital
2001
Royal Marsden Hospital
1984-1987
Ludwig Cancer Research
1984
Royal Marsden NHS Foundation Trust
1984
University of Warwick
1979-1980
Abstract X‐box binding protein 1 (XBP‐1) is stimulated by endoplasmic reticulum stress as part of the unfolded response (UPR), which can promote apoptosis or cell survival. Non‐conventional splicing, during UPR, converts mRNA for “unspliced” XBP‐1U to “spliced” XBP‐1S mRNA. XBP‐1 oestrogen‐responsive, but confers oestrogen independence and anti‐oestrogen resistance breast cancer lines. We therefore evaluated splicing a factor in patients endocrine treatment. isoforms were measured...
Abstract Osteoporosis is the most common age-related bone disease worldwide and usually clinically asymptomatic until first fracture happens. MicroRNAs are critical molecular regulators in remodelling processes stabilised blood. The aim of this project was to identify circulatory microRNAs associated with osteoporosis using advanced PCR arrays initially identified differentially-expressed were validated clinical samples RT-qPCR. A total 161 participants recruited 139 included study local...
Abstract S100P, an EF-hand calcium-binding protein, has been reported to be associated with the progression of many types cancers. Transfection expression vector for S100P into a benign, nonmetastatic rat mammary cell line causes 4- 6-fold increase in its level all four transformant clones. When resultant lines are introduced turn fat pads syngeneic Furth-Wistar rats, there is significant 3-fold local muscle invasion and induction metastasis 64% 75% tumor-bearing animals. In group 303 breast...
The anterior gradient protein-2 (AGR2) is inducible by oestrogen and itself can induce metastasis in a rat model for breast cancer. Here, rabbit antibody to recombinant human AGR2 was used assess its prognostic significance retrospective cohort of 351 cancer patients treated adjuvant hormonal therapy. stains 66% carcinomas varying degrees. percentage positive carcinoma cells tumours directly correlates with the level mRNA (Spearman's rank correlation, P=0.0007) protein (linear regression...
Abstract The calcium‐binding protein S100A4 induces the metastatic phenotype in rodent models of breast cancer and its expression correlates strongly with reduced survival human cancer. normal bladders 101 bladder tumours has been studied using immunocytochemistry. Moderate or strong was found 28% tumours, whilst remaining urothelium either failed to stain showed weak staining. staining more frequently observed invasive than non‐invasive ( p <0.05). In usually strongest regions single...
Elevated levels of the calcium-binding protein S100A4 are associated with poor patient survival in breast cancer patients and induce metastasis rodent models. To investigate effects on different components metastatic process, epithelial cells lines have been isolated from nonmalignant tumours neu transgenic mice malignant neu/S100A4 double mice. Additional cell expressing both Neu also derived by transfection rat cDNA into tumour cloned single Using these transfilter migration assays, it has...
A suppression subtraction cDNA library representing mRNAs expressed at a higher level in benign breast tumour-derived cell line relative to the malignant MCF-7A contained cDNAs corresponding for plasminogen activator inhibitor I, annexin VIII and EF-hand protein S100A2. S100A2 has previously been shown be normal human epithelium, but not carcinoma lines. Using PCR-based assay situ hybridization on histological sections of specimens, mRNA was present all lesions examined as well epithelium....
Abstract PURPOSE: S100A4 and the estrogen-inducible osteopontin are alone capable of inducing angiogenesis metastasis in rodent models for breast cancer. The present study assesses relationship with vessel density estrogen receptor alpha (ERalpha) primary tumors survival patients to ascertain their involvement metastatic EXPERIMENTAL DESIGN: Primary from 312 treated minimally invasive human cancer were immunocytochemically stained then assessed significance association each other using...
S100 proteins promote cancer cell migration and metastasis. To investigate their roles in the process of we have constructed inducible systems for S100P rat mammary human HeLa cells that show a linear relationship between its intracellular levels migration. S100P, like S100A4, differentially interacts with isoforms nonmuscle myosin II (NMIIA, Kd = 0.5 μm; IIB, 8 IIC, 1.0 μm). Accordingly, dissociates NMIIA IIC filaments but not IIB vitro. knockdown increases non-induced there is no further...
Anterior gradient 2 (AGR2) is a normal endoplasmic reticulum protein that has two important abnormal functions, amphibian limb regeneration and human cancer metastasis promotion. These intracellular extracellular roles can be attributed to the multidomain structure of AGR2. The NMR shows AGR2 consists an unstructured N-terminal region followed by thioredoxin fold. exists in monomer–dimer equilibrium with Kd 8.83 μM, intermolecular salt bridges involving E60 K64 within folded domain serve...