A5018380234- S100 Proteins and Annexins
- RNA and protein synthesis mechanisms
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- RNA Research and Splicing
- Biotin and Related Studies
- Fungal and yeast genetics research
- Blood properties and coagulation
- Protease and Inhibitor Mechanisms
- Tendon Structure and Treatment
- RNA modifications and cancer
- ATP Synthase and ATPases Research
- Neonatal Respiratory Health Research
- Reproductive System and Pregnancy
- Cell death mechanisms and regulation
- Chemical Synthesis and Analysis
- Pregnancy and preeclampsia studies
- Bacterial Genetics and Biotechnology
- Vitamin D Research Studies
- Pregnancy and Medication Impact
- Immune Response and Inflammation
- Nuclear Structure and Function
- Aldose Reductase and Taurine
- Genomics and Phylogenetic Studies
- Bioactive Compounds and Antitumor Agents
Aston University
2010-2023
University of Birmingham
2022-2023
Royal Veterinary College
2022-2023
University College London
2022-2023
Institut de Recherche en Infectiologie de Montpellier
2023
Centre National de la Recherche Scientifique
2023
Johnson University
2003-2010
Fondazione Umberto Di Mario
2010
Liverpool John Moores University
2009
Rutgers, The State University of New Jersey
2005-2006
Increasing evidence suggests that tissue transglutaminase (tTGase; type II) is externalized from cells, where it may play a key role in cell attachment and spreading the stabilization of extracellular matrix (ECM) through protein cross-linking. However, relationship between these different functions enzyme's mechanism secretion not fully understood. We have investigated tTGase migration using two stably transfected fibroblast lines which expression its active inactive (C277S mutant) states...
Release of cytochrome c from mitochondria is a major event during apoptosis. Released has been shown to activate caspase-dependent apoptotic signals. In this report, we provide evidence for novel role in caspase-independent nuclear We showed that c, released upon apoptosis induction, gradually accumulates the nucleus as evidenced by both immunofluorescence and subcellular fractionation. Parallel accumulation acetylated histone H2A, but not unmodified was cytoplasm. Addition purified isolated...
Using a cytochemical approach, we examined the role of tissue transglutaminase (tTgase, Type II) in incorporation latent TGF-β binding protein-1 (LTBP-1) extracellular matrix Swiss 3T3 fibroblasts which tTgase expression can be modulated through tetracycline-controlled promoter. Increased led to an increased rate LTBP-1 deposition matrix, was accompanied by pool deoxycholate-insoluble fibronectin. Matrix could also reduced competitive amine substrate putrescine. Immunolocalization at...
Although the actin cytoskeleton and translation machinery are considered to be separate cellular complexes, growing evidence supports overlapping regulation of two systems. Because its interaction with actin, eukaryotic elongation factor 1A (eEF1A) is proposed a regulator or link between these processes. Using genetic approach yeast Saccharomyces cerevisiae, specific regions eEF1A responsible for interactions bundling were identified. Five new mutations identified along one face eEF1A....
The dipeptide carnosine (β-alanyl-L-histidine) has contrasting but beneficial effects on cellular activity. It delays senescence and rejuvenates cultured senescent mammalian cells. However, it also inhibits the growth of tumour Based studies in several organisms, we speculate that exerts these apparently opposing actions by affecting energy metabolism and/or protein homeostasis (proteostasis). Specific include dipeptide's influence ATP concentrations. Carnosine's ability to reduce formation...
S100 proteins promote cancer cell migration and metastasis. To investigate their roles in the process of we have constructed inducible systems for S100P rat mammary human HeLa cells that show a linear relationship between its intracellular levels migration. S100P, like S100A4, differentially interacts with isoforms nonmuscle myosin II (NMIIA, Kd = 0.5 μm; IIB, 8 IIC, 1.0 μm). Accordingly, dissociates NMIIA IIC filaments but not IIB vitro. knockdown increases non-induced there is no further...
// EL-Habib Dakir 1, 2, 8 , Adam Pickard 1 Kirtiman Srivastava Cian M. McCrudden 3 Stephane R. Gross 4 Stephen Lloyd 5 Shu-Dong Zhang 6 Andriana Margariti 7 Richard Morgan Philip S. Rudland 9 and Mohamed El-Tanani Center for Cancer Research Cell Biology, Queen's University, Belfast, UK 2 Instituto de Biología Molecular y Celular del Cáncer, Centro Investigación Consejo Superior Investigaciones Científicas (CSIC), Universidad Salamanca, Spain School of Pharmacy, University Life Health...
The region of tenascin-C containing only alternately spliced fibronectin type-III repeat D (fnD) increases neurite outgrowth by itself and also as part tenascin-C. We previously localized the active site within fnD to an eight amino acid sequence unique tenascin-C, VFDNFVLK, showed that acids FD FV are required for activity. purpose this study was identify neuronal receptor interacts with VFDNFVLK investigate hypothesis important binding. Function-blocking antibodies against both α7 β1...
Eukaryotic initiation factor 2A (eIF2A) has been shown to direct binding of the initiator methionyl-tRNA (Met-tRNA(i)) 40 S ribosomal subunits in a codon-dependent manner, contrast eIF2, which requires GTP but not AUG codon bind tRNA subunits. We show here that yeast eIF2A genetically interacts with eIF4E, suggesting both proteins function same pathway. The double eIF2A/eIF4E-ts mutant strain displays severe slow growth phenotype, correlated accumulation 85% cells arrested at G(2)/M border....
Aquaporins are membrane proteins that regulate cellular water flow. Recently, aquaporins have been proposed as mediators of cancer cell biology. A subset aquaporins, referred to aquaglyceroporins known facilitate the transport glycerol. The present study describes effect gene knockdown aquaglyceroporin AQP3 on MDA-MB-231 breast proliferation, migration, invasion, adherence and response chemotherapeutic agent 5-fluorouracil. shRNA mediated induced a 28% reduction in proliferation (P<0.01),...
Elevated levels of the calcium-binding protein S100A4 promote metastasis and in carcinoma cells are associated with reduced survival cancer patients. interacts target proteins that affect a number activities metastatic cells. However, it is not known how many these interactions required for S100A4-promoted metastasis, thus hampering design specific inhibitors S100A4-induced metastasis. Intracellular exists as homodimer through previously identified, well conserved, predominantly hydrophobic...
The protein ezrin has been shown to enhance cancer cell motility and invasion leading malignant behaviours in solid tumours, but a similar regulatory function the early physiological reproduction state is, however, much less clear. We speculated that may play key role promoting first-trimester extravillous trophoblast (EVT) migration/invasion. Ezrin, as well its Thr567 phosphorylation, were found all trophoblasts studied, whether primary cells or lines. Interestingly, proteins seen distinct...
Eukaryotic translation elongation factor 3 (eEF3) is a fungal-specific ATPase proposed to catalyze the release of deacylated-tRNA from ribosomal E-site. In addition, it has been shown interact with aminoacyl-tRNA binding GTPase 1A (eEF1A), perhaps linking E and A sites. Domain mapping demonstrates that amino acids 775-980 contain eEF1A III eEF1A, which also involved in actin-related functions, site eEF3 binding. The enhanced by ADP, indicating interaction favored post-ATP hydrolysis but not...
S100P has been shown to be a marker for carcinogenesis where its expression in solid tumours correlates with metastasis and poor patient prognosis. This protein's role any physiological process is, however, unknown. Here we first show that is expressed both trophoblasts vivo as well some corresponding cell lines culture. We demonstrate predominantly during the early stage of placental formation highest levels occurring trimester gestation, particularly invading columns anchoring villi. Using...
Membrane proteins are drug targets for a wide range of diseases. Having access to appropriate samples further research underpins the pharmaceutical industry's strategy developing new drugs. This is typically achieved by synthesizing protein interest in host cells that can be cultured on large scale, allowing isolation pure quantities much higher than those found protein's native source. Yeast popular as it eukaryote with similar synthetic machinery human source many interest, while also...
Early pregnancy is characterised by elevated circulating levels of vitamin D binding protein (DBP). The impact this on maternal and fetal health unclear but DBP present in the placenta, gene variants have been linked to malplacentation disorders such as preeclampsia. functional role placenta was investigated using trophoblastic JEG3, BeWo HTR8 cells. All three cell lines showed intracellular with increased expression nuclear localisation cells treated active form D, 1,25-dihydroxyvitamin...