A5085722901- Ion Transport and Channel Regulation
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Ion channel regulation and function
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Bacterial Genetics and Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Biofuel production and bioconversion
- Monoclonal and Polyclonal Antibodies Research
- Electrolyte and hormonal disorders
- Receptor Mechanisms and Signaling
- Lipid Membrane Structure and Behavior
- Carbohydrate Chemistry and Synthesis
- Membrane-based Ion Separation Techniques
- Protein purification and stability
- Transgenic Plants and Applications
- Drug Transport and Resistance Mechanisms
- Pancreatic function and diabetes
- Enzyme Production and Characterization
- Enzyme Structure and Function
- Chemical Reactions and Isotopes
- Neuropeptides and Animal Physiology
- Neonatal Respiratory Health Research
- Cellular transport and secretion
Aston University
2016-2025
University College Birmingham
2022
Imperial College London
2009
Chalmers University of Technology
2003-2006
University of Gothenburg
1998-2004
University of Edinburgh
1999-2001
University of Michigan
1996-1998
Dyson (United Kingdom)
1997
John Radcliffe Hospital
1995-1997
University of Oxford
1995-1997
Swelling of the brain or spinal cord (CNS edema) affects millions people every year. All potential pharmacological interventions have failed in clinical trials, meaning that symptom management is only treatment option. The water channel protein aquaporin-4 (AQP4) expressed astrocytes and mediates flux across blood-brain blood-spinal barriers. Here we show AQP4 cell-surface abundance increases response to hypoxia-induced cell swelling a calmodulin-dependent manner. Calmodulin directly binds...
The Concise Guide to PHARMACOLOGY 2021/22 is the fifth in this series of biennial publications. provides concise overviews, mostly tabular format, key properties nearly 1900 human drug targets with an emphasis on selective pharmacology (where available), plus links open access knowledgebase source and their ligands (www.guidetopharmacology.org), which more detailed views target ligand properties. Although constitutes over 500 pages, material presented substantially reduced compared...
Abstract Aquaporin channels facilitate bidirectional water flow in all cells and tissues. AQP4 is highly expressed astrocytes. In the CNS, it enriched astrocyte endfeet, at synapses, glia limitans, where mediates exchange across blood–spinal cord blood–brain barriers (BSCB/BBB), controls cell volume, extracellular space migration. Perivascular enrichment of BSCB/BBB suggests a role glymphatic function. Recently, we have demonstrated that localization also dynamically regulated subcellular...
The Concise Guide to PHARMACOLOGY 2023/24 is the sixth in this series of biennial publications. provides concise overviews, mostly tabular format, key properties approximately 1800 drug targets, and over 6000 interactions with about 3900 ligands. There an emphasis on selective pharmacology (where available), plus links open access knowledgebase source targets their ligands (https://www.guidetopharmacology.org/), which more detailed views target ligand properties. Although constitutes almost...
G-protein coupled receptors (GPCRs) constitute the largest class of membrane proteins and are a major drug target. A serious obstacle to studying GPCR structure/function characteristics is requirement extract from their native environment in plasma membrane, with inherent instability GPCRs detergents required for solubilization. In present study, we report first solubilization purification functional [human adenosine A2A receptor (A2AR)], total absence detergent at any stage, by exploiting...
Several host systems are available for the production of recombinant proteins, ranging from Escherichia coli to mammalian cell-lines. This article highlights benefits using yeast, especially more challenging targets such as membrane proteins. On account wide range molecular, genetic, and microbiological tools available, use well-studied model organism, Saccharomyces cerevisiae, provides many opportunities optimize functional yields a target protein. Despite this wealth resources, it is...
The aquaporin family of integral membrane proteins is composed channels that mediate cellular water flow. Aquaporin 4 (AQP4) highly expressed in the glial cells central nervous system and facilitates osmotically driven pathological brain swelling associated with stroke traumatic injury. Here we show AQP4 cell surface expression can be rapidly reversibly regulated response to changes tonicity primary cortical rat astrocytes transfected HEK293 cells. translocation mechanism involves PKA...
Abstract Human aquaporin 4 ( AQP 4) is the primary water channel protein in brain astrocytes. Hypothermia known to cause astrocyte swelling culture, but precise role of this process unknown. Primary human cortical astrocytes were cultured under hypothermic (32 °C) or normothermic (37 conditions. transcript, total and surface‐localized quantified using RT ‐ qPCR , sandwich ELISA with whole cell lysates surface biotinylation, followed by analysis protein, respectively. Four‐hour mild treatment...
ABSTRACT The yeast Saccharomyces cerevisiae predominantly ferments glucose to ethanol at high external concentrations, irrespective of the presence oxygen. In contrast, low concentrations and in oxygen, as a glucose-limited chemostat, no is produced. importance concentration suggests central role for affinity maximal transport rates yeast's transporters control production. Here we present series strains producing functional chimeras between hexose Hxt1 Hxt7, each which has distinct...
The control of cellular water flow is mediated by the aquaporin (AQP) family membrane proteins. structural features and mechanism selective passage through AQP pore are established, but there remains a gap in our knowledge how transport regulated. Two broad possibilities exist. One controlling pore, this only has been observed as phenomenon some plant microbial AQPs. An alternative number AQPs cell membrane. Here, we describe novel pathway mammalian cells whereby hypotonic stimulus directly...