A5024646408- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Fibroblast Growth Factor Research
- Axon Guidance and Neuronal Signaling
- RNA Interference and Gene Delivery
- Growth Hormone and Insulin-like Growth Factors
- Glaucoma and retinal disorders
- Corneal Surgery and Treatments
- Retinal Diseases and Treatments
- Proteoglycans and glycosaminoglycans research
- Retinal Development and Disorders
- Virus-based gene therapy research
- Traumatic Brain Injury and Neurovascular Disturbances
- Wound Healing and Treatments
- Retinal and Macular Surgery
- Ocular Surface and Contact Lens
- Traumatic Ocular and Foreign Body Injuries
- Cell death mechanisms and regulation
- S100 Proteins and Annexins
- Cancer, Hypoxia, and Metabolism
- TGF-β signaling in diseases
- Signaling Pathways in Disease
- Intraocular Surgery and Lenses
- Skin Protection and Aging
- Mesenchymal stem cell research
University of Warwick
2021-2025
Teer Coatings (United Kingdom)
2021-2024
University Dermatology
2023
University of Birmingham
2012-2022
NIHR Surgical Reconstruction and Microbiology Research Centre
2012-2020
University Hospitals Birmingham NHS Foundation Trust
2020
The Edgbaston Hospital
2018-2019
Queen Elizabeth Hospital Birmingham
1990-2017
Queen Elizabeth Hospital
1995-2017
National Institute for Health Research
2012-2017
Swelling of the brain or spinal cord (CNS edema) affects millions people every year. All potential pharmacological interventions have failed in clinical trials, meaning that symptom management is only treatment option. The water channel protein aquaporin-4 (AQP4) expressed astrocytes and mediates flux across blood-brain blood-spinal barriers. Here we show AQP4 cell-surface abundance increases response to hypoxia-induced cell swelling a calmodulin-dependent manner. Calmodulin directly binds...
We have investigated and compared the neurotrophic activity of human dental pulp stem cells (hDPSC), bone marrow-derived mesenchymal (hBMSC) adipose-derived (hAMSC) on axotomised adult rat retinal ganglion (RGC) in vitro order to evaluate their therapeutic potential for neurodegenerative conditions RGC. Using transwell system, RGC survival length/number neurites were quantified coculture with presence or absence specific Fc-receptor inhibitors determine role NGF, BDNF, NT-3, VEGF, GDNF,...
Purpose.: To investigate the potential therapeutic benefit of intravitreally implanted dental pulp stem cells (DPSCs) on axotomized adult rat retinal ganglion (RGCs) using in vitro and vivo neural injury models. Methods.: Conditioned media collected from cultured DPSCs bone marrow–derived mesenchymal (BMSCs) were assayed for nerve growth factor (NGF), brain-derived neurotrophic (BDNF), neurotrophin-3 (NT-3) secretion ELISA. or BMSCs cocultured with cells, without Fc-TrK inhibitors, a...
Abstract In the central nervous system (CNS), nerve regeneration after traumatic injury fails. The formation of a dense fibrous scar is thought to restrict in part growth axonal projections, providing one many reasons that complete lesions neural pathways adult mammalian CNS are rarely followed by significant functional recovery. order determine which mechanisms mediate and investigate whether they can be modulated vivo , we have attempted define potential role trophic factors. Our previous...
Traumatic injury to the CNS initiates transient and unsuccessful regeneration of damaged neural pathways, accompanied by reactive gliosis, angiogenesis, deposition a dense fibrous glial/meningeal scar at wound site. Basic fibroblast growth factor (basic FGF) is protein with potent effects on neurons, glia, fibroblasts, vascular endothelial cells. Hybridization immunocytochemical methods were used examine temporal spatial changes in distribution levels basic FGF mRNA also its receptor (flg),...
Synthetic vectors based on reducible polycations consisting of histidine and polylysine residues (HIS RPCs) were evaluated for their ability to deliver nucleic acids. Initial experiments showed that RPC-based with at least 70% content mediated efficient levels gene transfer without requirement the endosomolytic agent chloroquine. Significant was observed in a range cell types achieving up 5-fold increase percentage transfected cells compared 25 kDa PEI, gold standard synthetic vector. In...
Traumatic brain injury (TBI) is the leading cause of death and disability in people younger than 45 Western countries. Despite many studies, no reliable biomarkers have been found to assess TBI severity predict recovery. MicroRNA (miRNA) profiling has become widely used identify therapeutic targets. Through use TaqMan Array Human A+B Cards, expression 754 miRNAs was analyzed serum five mild (mTBI) patients with extra-cranial (EC), severe (sTBI) EC, healthy volunteers (HV) at 1 day 15 days...
This review will describe the unique advantages that are offered by visual system of mammals and other vertebrates for studying regenerative responses central nervous (CNS) to injury, recent insights provided such studies. In mouse rat a variety experimental paradigms promote survival retinal ganglion cells (RGC) optic nerve regeneration, probably through stimulation neurotrophic factors (NTF) either directly, or indirectly astrocyte/Müller cell intermediary activation. NTF induce...
Retinal ganglion cell (RGC) loss after optic nerve damage is a hallmark of certain human ophthalmic diseases including ischemic neuropathy (ION) and glaucoma. In rat model transection, in which 80% RGCs are eliminated within 14 days, caspase-2 was found to be expressed cleaved (activated) predominantly RGC. Inhibition expression by chemically modified synthetic short interfering ribonucleic acid (siRNA) delivered intravitreal administration significantly enhanced RGC survival over period at...
Concussion is difficult to diagnose, particularly when and symptoms are atypical or late in presenting. An accurate timely initial assessment crucial for clinical management. Cerebral spinal fluid (CSF) blood markers of traumatic brain injury show promising results but their applicability concussion has significant limitations. In the study, we explored saliva as a new source biomarkers concussion. Saliva samples concussed players were collected after 48-72 hours from analyzed by...
We have demonstrated a 50-fold increase in the concentration of insulin-like growth factor-binding protein-5 (IGFBP-5) milk after 2 days mammary involution induced by removal suckling young. IGFBP-5 was identified its immunoreactivity with an antiserum to and shown situ hybridization be synthesized secretory epithelial cells undergoing apoptosis. Smaller increases IGFBP-2 -4 messenger RNAs (mRNAs) were also evident, but neither protein could detected on Western ligand blots milk. Preliminary...
The therapeutic effects of individual neurotrophic factors (NTF) have proved disappointing in clinical trials for neuronal repair and axon regeneration. Here, we demonstrate NTF synergistic responses after a combination basic fibroblast growth factor, neurotrophin-3 brain derived factor delivered to the somata retinal ganglion cells promoted greater survival than did sum each alone. Triple not single treatments potentiated regulated intramembraneous proteolysis p75(NTR), ectodomain shedding...
In post-haemorrhagic and other forms of communicating hydrocephalus, cerebrospinal fluid flow drainage is obstructed by subarachnoid fibrosis in which the potent fibrogenic cytokine transforming growth factor-β has been aetiologically implicated. Here, hypothesis that antagonist decorin therapeutic potential for reducing ventriculomegaly was tested using a rat model juvenile hydrocephalus. Hydrocephalus induced single basal cistern injection kaolin 3-week-old rats, immediately followed 3 or...
PURPOSE.To evaluate the efficacy of anti-VEGF agents for treating choroidal neovascularization (CNV) when delivered topically using novel cell-penetrating peptides (CPPs) compared with delivery by intravitreal (ivit) injection.METHODS.CPP toxicity was investigated in cell cultures.Ivit concentrations ranibizumab and bevacizumab after topical administration were measured ELISA.The biological þ CPP complexes ivit injections an established model CNV.RESULTS.CPPs nontoxic vitro.In vivo, eye drop...