A5038629041- Advancements in Transdermal Drug Delivery
- Immunotherapy and Immune Responses
- Advanced Drug Delivery Systems
- RNA Interference and Gene Delivery
- Dermatology and Skin Diseases
- Advanced Biosensing Techniques and Applications
- Particle Dynamics in Fluid Flows
- Monoclonal and Polyclonal Antibodies Research
- Microfluidic and Bio-sensing Technologies
- Bee Products Chemical Analysis
- 3D Printing in Biomedical Research
- Herpesvirus Infections and Treatments
- Intramuscular injections and effects
- Biosensors and Analytical Detection
- Advanced Fluorescence Microscopy Techniques
- Transgenic Plants and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Textile materials and evaluations
- Cellular Mechanics and Interactions
- Lipid Membrane Structure and Behavior
- Toxin Mechanisms and Immunotoxins
- Fluid Dynamics and Turbulent Flows
- Granular flow and fluidized beds
- Microfluidic and Capillary Electrophoresis Applications
- Tendon Structure and Treatment
The University of Queensland
2012-2022
Royal Brisbane and Women's Hospital
2014-2019
Australian National University
2018-2019
Vaxxas (Australia)
2013-2019
Translational Research Institute
2014-2019
Australian e-Health Research Centre
2014-2018
Princess Alexandra Hospital
2010-2016
University of Oxford
2000-2007
Science Oxford
2004-2006
Plymouth Marine Laboratory
1990
Emerging micro-scale medical devices are showing promise, whether in delivering drugs or extracting diagnostic biomarkers from skin. In progressing these through animal models towards clinical products, understanding the mechanical properties and skin tissue structure with which they interact will be important. Here, measurement analytical modelling, we advanced knowledge of for commonly used laboratory animals humans (~30 g to ~150 kg). We hypothesised that skin's stiffness is a function...
Synthetic vectors based on reducible polycations consisting of histidine and polylysine residues (HIS RPCs) were evaluated for their ability to deliver nucleic acids. Initial experiments showed that RPC-based with at least 70% content mediated efficient levels gene transfer without requirement the endosomolytic agent chloroquine. Significant was observed in a range cell types achieving up 5-fold increase percentage transfected cells compared 25 kDa PEI, gold standard synthetic vector. In...
Background Over 14 million people die each year from infectious diseases despite extensive vaccine use [1]. The needle and syringe—first invented in 1853—is still the primary delivery device, injecting liquid into muscle. Vaccines could be far more effective if they were precisely delivered narrow layer just beneath skin surface that contains a much higher density of potent antigen-presenting cells (APCs) essential to generate protective immune response. We hypothesized successful...
Abstract The ‘Nanopatch’ (NP) comprises arrays of densely packed projections with a defined geometry and distribution designed to physically target vaccines directly thousands epidermal dermal antigen presenting cells (APCs). These miniaturized are two orders magnitude smaller than standard needles—which deliver most vaccines—and also much current microneedle arrays. NP is dry‐coated antigen, adjuvant, and/or DNA payloads. After the was pressed onto mouse skin, protein payload co‐localized...
Abstract Targeting of vaccines to abundant immune cell populations within our outer thin skin layers using miniaturized devices—much thinner than a needle and syringe, could improve the efficacy (and other immunotherapies). To meet this goal, densely packed dissolving microprojection array (dissolving Nanopatch) is designed, achieving functional miniaturization by 1) formulating small microneedles (two orders magnitude smaller standard syringe) 2) multiple layering payload microprojections...
Systematic studies probing the effects of nanoparticle surface modification and formulation pH are important in nanotoxicology nanomedicine. In this study, we use laser-scanning fluorescence confocal microscopy to evaluate penetration viable excised human skin that was intact or tape-stripped. Quantum dot (QD) fluorescent nanoparticles with three modifications: Polyethylene glycol (PEG), PEG-amine (PEG-NH2) PEG-carboxyl (PEG-COOH) were evaluated for from aqueous solutions at 7.0 pHs provided...
Background Better delivery systems are needed for routinely used vaccines, to improve vaccine uptake. Many vaccines contain alum or based adjuvants. Here we investigate a novel dry-coated densely-packed micro-projection array skin patch (Nanopatch™) as an alternate system intramuscular injection delivering adjuvanted human papillomavirus (HPV) (Gardasil®) commonly prophylactic against cervical cancer. Methodology/Principal Findings Micro-projection arrays with material delivered C57BL/6...
The disadvantages of needle-based immunisation motivate the development simple, low cost, needle-free alternatives. Vaccine delivery to cutaneous environments rich in specialised antigen-presenting cells using microprojection patches has practical and immunological advantages over conventional needle delivery. Additionally, stable coating vaccine onto microprojections removes logistical obstacles presented by strict requirement for cold-chain storage distribution liquid vaccine, or...
While advances in assay chemistry and detection continue to improve molecular diagnostics technology, blood samples are still collected using the 150-year-old needle/syringe method. Surface modified microprojection arrays have been developed as a novel platform for vivo, needle-free biomarker capture. These devices gold coated silicon with >20,000 projections per cm2, which can be applied skin tunable penetration into epidermis or dermis. The array conceptually offers several advantages over...
Minimally invasive biosensors are of great interest for rapid detection disease biomarkers diagnostic screening at the point-of-care. Here we introduce a device which extracts disease-specific directly from upper dermis, without needle and syringe or resource-intensive blood processing. Using antigen-specific antibodies raised in mice as model system, confirm analytical specificity sensitivity antibody capture extraction comparison to conventional methods based on needle/syringe draw...