A5035480938- Advanced Biosensing Techniques and Applications
- Advancements in Transdermal Drug Delivery
- Biosensors and Analytical Detection
- Microfluidic and Bio-sensing Technologies
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Dermatology and Skin Diseases
- Contact Dermatitis and Allergies
- Viral gastroenteritis research and epidemiology
- Wound Healing and Treatments
- Glycosylation and Glycoproteins Research
- Transgenic Plants and Applications
- Chemical Synthesis and Analysis
- Intramuscular injections and effects
- Advanced Fiber Optic Sensors
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Nanofabrication and Lithography Techniques
- Polymer Surface Interaction Studies
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Biotin and Related Studies
- Surface Modification and Superhydrophobicity
The University of Queensland
2012-2022
Massachusetts Institute of Technology
2018-2022
Brigham and Women's Hospital
2020-2022
Harvard University
2020-2022
Peter Doherty Institute
2020-2022
The University of Melbourne
2020-2022
UNSW Sydney
2009
Touro Infirmary Foundation
1966
While advances in assay chemistry and detection continue to improve molecular diagnostics technology, blood samples are still collected using the 150-year-old needle/syringe method. Surface modified microprojection arrays have been developed as a novel platform for vivo, needle-free biomarker capture. These devices gold coated silicon with >20,000 projections per cm2, which can be applied skin tunable penetration into epidermis or dermis. The array conceptually offers several advantages over...
Herein we demonstrate the use of a wearable device that can selectively capture two distinct circulating protein biomarkers (recombinant P. falciparum rPfHRP2 and total IgG) from intradermal fluid live mice in situ, for subsequent detection vitro. The comprises microprojection array that, when applied to skin, penetrates outer skin layers interface directly with fluid. Because complexity biological being sampled, investigated effects solution conditions on attachment antibodies, optimize...
Micro-device use for vaccination has grown in the past decade, with promise of ease-of-use, painless application, stable solid formulations and greater immune response generation. However, designs highly immunogenic devices (e.g. gene gun, Nanopatch or laser adjuvantation) require significant energy to enter skin (30-90 mJ). Within this study, we explore a way more effectively penetration vaccination. These modifications change projections from cylindrical/conical shapes density 20,000 per...
Herein, we report the fabrication, characterization, and testing of a polymer microprojection array, for direct selective capture circulating biomarkers from skin live mice. First, modified polycarbonate wafers using an electrophilic aromatic substitution reaction with nitric acid to insert nitro-groups into benzene rings, followed by treatment sodium borohydride reduce primary amines. Initial characterization ultraviolet-visible (UV-vis) spectroscopy suggested that increasing concentration...
Selective capture of disease-related proteins in complex biological fluids and tissues is an important aim developing sensitive protein biosensors for vivo applications. Microprojection arrays are biomedical devices whose mechanical chemical properties can be tuned to allow efficient penetration skin, coupled with highly selective biomarker from the environment skin tissue. Herein, authors describe improved surface modification strategy produce amine-modified polycarbonate arrays, followed...
Many types of microneedle (MN) arrays have been tested for delivery vaccines to the skin. However, effect MN geometry/array design on antibody production is still unclear. Reports suggest that systemic immune responses may be affected by how “mechanically” deliver vaccines, which can induce cell damage and act as an adjuvant. This includes parameters such length/insertion depth, energy/velocity, shape, density. these effects not systematically investigated. Herein, density influenza...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelf-Cleaning Surfaces: A Third-Year Undergraduate Research ProjectRonald S. Haines , Alex H. F. Wu Hua Zhang Jacob Coffey Thomas Huddle Justin Lafountaine Zhi-Jun Lim Eugene A. White Nam T. Tuong and Robert N. Lamb View Author Information School of Chemistry, University New South Wales, Sydney, NSW 2052, Australia Melbourne, VIC 3010, AustraliaCite this: J. Chem. Educ. 2009, 86, 3, 365Publication Date (Web):March 1, 2009Publication History...
Immunoassays are ubiquitous across research and clinical laboratories, yet little attention is paid to the effect of substrate material on assay performance characteristics. Given emerging interest in wearable immunoassay formats, investigations into materials that provide an optimal mix mechanical bioanalytical properties paramount. In course our developing immunoassays which can penetrate skin selectively capture disease antigens from underlying blood vessels, we recently identified...
Summary The administration of S-180 mouse ascites tumor cells to rats has been shown stimulate the production two distinct lysins capable rupturing in vitro. antibodies were separated on diethylaminoethyl (DEAE)-cellulose and by sucrose density gradient centrifugation chromatography Sephadex G-200 be 7 S antibodies. No 19 antibody was detectable either day or 14. data are consistent with theory that there is a sequential synthesis lysin which predominates serum 14 identified as γSS-globulin...
Whilst blood is the sample most often collected for diagnostic purposes, testing complicated by need to purify or concentrate biomarkers prior detection. While needle/syringe lancet technology used bulk collection, devices have not yet been developed that selectively capture of interest in vivo, simplifying downstream detection requirements. Our group Micropatch Array — a device comprising an array microprojections which breach outer layers skin from dermis. In this presentation we will...