A5004194487- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Axon Guidance and Neuronal Signaling
- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Neonatal and fetal brain pathology
- Glaucoma and retinal disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- RNA Interference and Gene Delivery
- Fibroblast Growth Factor Research
- Corneal Surgery and Treatments
- Cerebrospinal fluid and hydrocephalus
- Mesenchymal stem cell research
- Virus-based gene therapy research
- Spinal Cord Injury Research
- Retinal Diseases and Treatments
- Vestibular and auditory disorders
- Retinal and Macular Surgery
- Mitochondrial Function and Pathology
- Wound Healing and Treatments
- S100 Proteins and Annexins
- Anesthesia and Neurotoxicity Research
- Neural dynamics and brain function
- Traumatic Ocular and Foreign Body Injuries
University of Birmingham
2012-2021
University College Birmingham
2014
NIHR Birmingham Biomedical Research Centre
2011
Queen Elizabeth Hospital Birmingham
2005-2007
King's College London
1999-2006
Guy's Hospital
1990-2004
Berry (United States)
2004
Wellcome Centre for Human Neuroimaging
2001-2002
St Thomas' Hospital
1989-1998
Institute of Cell Biology and Neurobiology
1997
We have investigated and compared the neurotrophic activity of human dental pulp stem cells (hDPSC), bone marrow-derived mesenchymal (hBMSC) adipose-derived (hAMSC) on axotomised adult rat retinal ganglion (RGC) in vitro order to evaluate their therapeutic potential for neurodegenerative conditions RGC. Using transwell system, RGC survival length/number neurites were quantified coculture with presence or absence specific Fc-receptor inhibitors determine role NGF, BDNF, NT-3, VEGF, GDNF,...
Purpose.: To investigate the potential therapeutic benefit of intravitreally implanted dental pulp stem cells (DPSCs) on axotomized adult rat retinal ganglion (RGCs) using in vitro and vivo neural injury models. Methods.: Conditioned media collected from cultured DPSCs bone marrow–derived mesenchymal (BMSCs) were assayed for nerve growth factor (NGF), brain-derived neurotrophic (BDNF), neurotrophin-3 (NT-3) secretion ELISA. or BMSCs cocultured with cells, without Fc-TrK inhibitors, a...
Abstract In the central nervous system (CNS), nerve regeneration after traumatic injury fails. The formation of a dense fibrous scar is thought to restrict in part growth axonal projections, providing one many reasons that complete lesions neural pathways adult mammalian CNS are rarely followed by significant functional recovery. order determine which mechanisms mediate and investigate whether they can be modulated vivo , we have attempted define potential role trophic factors. Our previous...
Synthetic vectors based on reducible polycations consisting of histidine and polylysine residues (HIS RPCs) were evaluated for their ability to deliver nucleic acids. Initial experiments showed that RPC-based with at least 70% content mediated efficient levels gene transfer without requirement the endosomolytic agent chloroquine. Significant was observed in a range cell types achieving up 5-fold increase percentage transfected cells compared 25 kDa PEI, gold standard synthetic vector. In...
The NG2 antibody, which recognises an integral membrane chondroitin sulphate, labels a significant population of cells in adult CNS white matter tracts the rat optic nerve and anterior medullary velum (AMV). Adult NG2+ are highly complex with multiple branching processes we show by EM immunocytochemistry that they extend perinodal processes, contact nodes Ranvier. do not react to conventional immunohistochemical markers for glia so reservedly term them NG2P cells. In vitro,...
This review will describe the unique advantages that are offered by visual system of mammals and other vertebrates for studying regenerative responses central nervous (CNS) to injury, recent insights provided such studies. In mouse rat a variety experimental paradigms promote survival retinal ganglion cells (RGC) optic nerve regeneration, probably through stimulation neurotrophic factors (NTF) either directly, or indirectly astrocyte/Müller cell intermediary activation. NTF induce...
The therapeutic effects of individual neurotrophic factors (NTF) have proved disappointing in clinical trials for neuronal repair and axon regeneration. Here, we demonstrate NTF synergistic responses after a combination basic fibroblast growth factor, neurotrophin-3 brain derived factor delivered to the somata retinal ganglion cells promoted greater survival than did sum each alone. Triple not single treatments potentiated regulated intramembraneous proteolysis p75(NTR), ectodomain shedding...