A5026031146- Cancer Genomics and Diagnostics
- Ocular Oncology and Treatments
- Molecular Biology Techniques and Applications
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- Neuroblastoma Research and Treatments
- Cancer Cells and Metastasis
- Lung Cancer Treatments and Mutations
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Gastrointestinal Tumor Research and Treatment
- Sarcoma Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Chromatin Remodeling and Cancer
- Ubiquitin and proteasome pathways
- Chemokine receptors and signaling
- Gene expression and cancer classification
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- 3D Printing in Biomedical Research
- Lung Cancer Research Studies
- Advanced biosensing and bioanalysis techniques
- Gastric Cancer Management and Outcomes
- Nanoplatforms for cancer theranostics
- Genomics and Phylogenetic Studies
University of Gothenburg
2016-2025
Sahlgrenska University Hospital
2016-2025
Region Västra Götaland
2021-2025
Wallenberg Wood Science Center
2019-2024
Varberg Hospital
2024
Medical University of Graz
2023
University of Graz
2023
University of Groningen
2020
University of Manchester
2019
Cancer Research UK Manchester Institute
2019
Reactive astrocytes are thought to protect the penumbra during brain ischemia, but direct evidence has been lacking due absence of suitable experimental models. Previously, we generated mice deficient in two intermediate filament (IF) proteins, glial fibrillary acidic protein (GFAP) and vimentin, whose upregulation is hallmark reactive astrocytes. GFAP −/− Vim exhibit attenuated posttraumatic gliosis, improved integration neural grafts, regeneration. Seven days after middle cerebral artery...
In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. We studied the reverse transcription reaction and its consequences for quantitative expression.We used SYBR green I-based real-time PCR (QPCR) to measure properties beta-tubulin, glyceraldehyde-3-phosphate dehydrogenase, Glut2, CaV1D, insulin II genes, using random hexamers, oligo(dT), gene-specific primers.Experimental variation in transcription-QPCR (RT-QPCR) was mainly attributable step. Reverse...
The transcriptional machinery in individual cells is controlled by a relatively small number of molecules, which may result stochastic behavior gene activity. Because technical limitations current collection and recording methods, most expression measurements are carried out on populations therefore reflect average mRNA levels. variability the transcript levels between different remains undefined, although it have profound effects cellular activities. Here we measured five genes ActB, Ins1,...
FUS, EWS and TAF15 are structurally similar multifunctional proteins that were first discovered upon characterization of fusion oncogenes in human sarcomas leukemias. The belong to the FET (previously TET) family RNA-binding implicated central cellular processes such as regulation gene expression, maintenance genomic integrity mRNA/microRNA processing. In present study, we investigated expression localization multiple tissues cell types. expressed both distinct overlapping patterns tissues....
Cell proliferation includes a series of events that is tightly regulated by several checkpoints and layers control mechanisms. Most studies have been performed on large cell populations, but detailed understanding dynamics heterogeneity requires single-cell analysis. Here, we used quantitative real-time PCR, profiling the expression 93 genes in single-cells from three different lines. Individual unsynchronized cells lines were collected cycle phases (G0/G1 - S G2/M) with variable sizes. We...
Abstract Preclinical studies have suggested that epigenetic therapy could enhance immunogenicity of cancer cells. We report the results PEMDAC phase 2 clinical trial ( n = 29; NCT02697630) where HDAC inhibitor entinostat was combined with PD-1 pembrolizumab in patients metastatic uveal melanoma (UM). The primary endpoint objective response rate (ORR), and met an ORR 14%. benefit at 18 weeks 28%, median progression free survival 2.1 months overall 13.4 months. Toxicities were manageable,...
In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. The reverse transcription reaction not very well understood, and it expected to be the uncertain step in expression analysis. It can introduce errors produced by effects secondary tertiary structures, variation priming efficiency, properties transcriptase (1)(2)(3)(4)(5). aim this work was study yield, reproducibility, sensitivity some commercially available transcriptases on low intermediate expressed...
Abstract Anaphylatoxin C3a is a third complement component (C3)-derived peptide, the multiple functions of which range from stimulation inflammation to neuroprotection. In previous study, we have shown that signaling through receptor positively regulates in vivo neurogenesis adult mouse brain. Here, studied direct effects on whole brain-derived neural progenitor cells (NPCs) vitro. Our results demonstrate NPCs bind specific and reversible manner stimulates neuronal differentiation NPCs....
Adult neurogenesis is regulated by a number of cellular players within the neurogenic niche. Astrocytes participate actively in brain development, regulation mature central nervous system (CNS), and plasticity. They are important regulators local environment adult niches through secretion diffusible morphogenic factors, such as Wnts. control niche also membrane-associated however, identity these factors mechanisms involved largely unknown. In this study, we sought to determine underlying our...
Astrocytes perform control and regulatory functions in the central nervous system; heterogeneity among them is still a matter of debate due to limited knowledge their gene expression profiles functional diversity. To unravel astrocyte during postnatal development after focal cerebral ischemia, we employed single-cell profiling acutely isolated cortical GFAP/EGFP-positive cells. Using microfluidic qPCR platform, profiled 47 genes encoding glial markers ion channels/transporters/receptors...
Detection of cell-free DNA in liquid biopsies offers great potential for use non-invasive prenatal testing and as a cancer biomarker. Fetal tumor fractions however can be extremely low these samples ultra-sensitive methods are required their detection. Here, we report an simple fast method introduction barcodes into libraries made from 5 ng DNA. Barcoded adapter primers designed with oligonucleotide hairpin structure to protect the molecular during first rounds polymerase chain reaction...
DDIT3, also known as GADD153 or CHOP, encodes a basic leucine zipper transcription factor of the dimer forming C/EBP family. DDIT3 is key regulator cellular stress response, but its target genes and functions are not well characterized. Here, we applied genome wide microarray based expression analysis to identify functions. By analyzing cells carrying tamoxifen inducible constructs show distinct gene profiles for with cytoplasmic nuclear localized DDIT3. Of 175 identified only 3 were...
Circulating cell-free tumor DNA (ctDNA) is a promising biomarker in cancer. Ultrasensitive technologies enable detection of low (< 0.1%) mutant allele frequencies, pre-requisite to fully utilize the potential ctDNA cancer diagnostics. In addition, entire liquid biopsy workflow needs be carefully optimized reliable analysis. Here, we discuss important considerations for plasma. We show how each experimental step can easily evaluated using simple quantitative PCR assays, including cellular...
Massively parallel sequencing technologies have long been used in both basic research and clinical routine. The recent introduction of digital has made previously challenging applications possible by significantly improving sensitivity specificity to now allow detection rare sequence variants, even at single molecule level. Digital utilizes unique molecular identifiers (UMIs) minimize sequencing-induced errors quantification biases. Here, we discuss the principles UMIs how they are...
Abstract Background: An abnormal IgLκ:IgLλ ratio has long been used as a clinical criterion for non-Hodgkin B-cell lymphomas. As first step toward quantitative real-time PCR-based multimarker diagnostic analysis of lymphomas, we have developed method determination in samples. Methods: Light-up probe-based PCR was to quantify IgLκ and IgLλ cDNA from 32 The samples were also investigated by routine immunohistochemical flow cytometry. Results: Of suspected lymphoma analyzed, 28 correctly...
Abstract Fibroblast growth factor (FGF) signaling controls axis formation during endoderm development. Studies in lower vertebrates have demonstrated that FGF2 primarily patterns the ventral foregut into liver and lung, whereas FGF4 exhibits broad anterior-posterior left-right patterning activities. Furthermore, an inductive role of dorsal pancreas has been shown. However, whether plays a similar human development remains unknown. Here, we show specifies hESC-derived definitive (DE)...
Gene expression has a strong stochastic element resulting in highly variable mRNA levels between individual cells, even seemingly homogeneous cell population. Access to fundamental information about cellular mechanisms, such as correlated gene expression, motivates measurements of multiple genes cells. Quantitative reverse transcription PCR (RT-qPCR) is the most accessible method which provides sufficiently accurate single Low concentration guanidine thiocyanate was used fully lyse...
Background Retinoic acid (RA) and fibroblast growth factor 4 (FGF4) signaling control endoderm patterning pancreas induction/expansion. Based on these findings, RA FGFs, excluding FGF4, have frequently been used in differentiation protocols to direct of hESCs into endodermal pancreatic cell types. In vivo, pathways act a temporal concentration-dependent manner. However, vitro, the underlying basis for time addition factors (GDFs), including as well concentration is lacking. Thus, order...
Single-cell gene expression levels show substantial variations among cells in seemingly homogenous populations. Astrocytes perform many control and regulatory functions the central nervous system. In contrast to neurons, we have limited knowledge about functional diversity of astrocytes its molecular basis. To study astrocyte heterogeneity stem/progenitor cell properties astrocytes, used single-cell profiling primary mouse dissociated neurosphere cells. The transcript number variability for...
Abstract Background: Quantitative PCR (qPCR) is a valuable technique for accurately and reliably profiling quantifying gene expression. Typically, samples obtained from the organism of study have to be processed via several preparative steps before qPCR. Method: We estimated errors sample withdrawal extraction, reverse transcription (RT), qPCR that are introduced into measurements mRNA concentrations. performed hierarchically arranged experiments with 3 animals, samples, RT reactions, qPCRs...