A5082766122- Microfluidic and Capillary Electrophoresis Applications
- Pancreatic function and diabetes
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Orthodontics and Dentofacial Orthopedics
- Neuroscience and Neural Engineering
- Silicon Nanostructures and Photoluminescence
- Temporomandibular Joint Disorders
- Biosensors and Analytical Detection
- Microfluidic and Bio-sensing Technologies
- Mass Spectrometry Techniques and Applications
- Social and Educational Sciences
- Molecular Biology Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Dental Radiography and Imaging
- Nanowire Synthesis and Applications
- Conducting polymers and applications
- 3D Printing in Biomedical Research
- Microbial Community Ecology and Physiology
- Nuclear reactor physics and engineering
- Enzyme Catalysis and Immobilization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nuclear Physics and Applications
- Single-cell and spatial transcriptomics
- Mycorrhizal Fungi and Plant Interactions
Lund University
2014-2025
Skåne University Hospital
2016-2024
Vattenfall (Sweden)
2016-2022
Swedish Nuclear Fuel and Waste Management (Sweden)
2016-2020
Karo Pharma (Sweden)
2004-2018
University of Gothenburg
2017
Medicon Village
2015
Oxford Centre for Diabetes, Endocrinology and Metabolism
2008-2014
Churchill Hospital
2008-2014
University of Oxford
2008-2014
OBJECTIVE— To characterize the voltage-gated ion channels in human β-cells from nondiabetic donors and their role glucose-stimulated insulin release. RESEARCH DESIGN AND METHODS— Insulin release was measured intact islets. Whole-cell patch-clamp experiments measurements of cell capacitance were performed on isolated β-cells. The channel complement determined by quantitative PCR. RESULTS— Human express two types K+ currents that flow through delayed rectifying (KV2.1/2.2) large-conductance...
The transcriptional machinery in individual cells is controlled by a relatively small number of molecules, which may result stochastic behavior gene activity. Because technical limitations current collection and recording methods, most expression measurements are carried out on populations therefore reflect average mRNA levels. variability the transcript levels between different remains undefined, although it have profound effects cellular activities. Here we measured five genes ActB, Ins1,...
Silicon microchips with immobilized antibodies were used to develop microfluidic enzyme immunoassays using chemiluminescence detection and horseradish peroxidase (HRP) as the label. Polyclonal anti-atrazine coupled silicon microchip surface an overall dimension of 13.1 × 3.2 mm, comprising 42 porous flow channels 235-μm depth 25-μm width. Different immobilization protocols based on covalent or noncovalent modification silica 3-aminopropyltriethoxysilane (APTES)...
Protein identification through peptide mass mapping by matrix-assisted laser desorption/ionization time-of-flight spectrometry (MALDI-TOF MS) has become a standard technique, used in many laboratories around the world. The traditional methodology often includes long incubations (6−24 h) and extensive manual steps. In an effort to address this, integrated microanalytical platform been developed for automated of proteins. silicon micromachined analytical tools, i.e., microchip immobilized...
OBJECTIVE Paracrine signaling via γ-aminobutyric acid (GABA) and GABAA receptors (GABAARs) has been documented in rodent islets. Here we have studied the importance of GABAergic human pancreatic RESEARCH DESIGN AND METHODS Expression GABAARs islet cells was investigated by quantitative PCR, immunohistochemistry, patch-clamp experiments. Hormone release measured from intact GABA monitored whole-cell measurements after adenoviral expression α1β1 GABAAR subunits. The subcellular localization...
Flexible neural probes are hypothesized to reduce the chronic foreign body response (FBR) mainly by reducing strain-stress caused an interplay between tethered probe and brain's micromotion. However, a large discrepancy of Young's modulus still exists (3-6 orders magnitude) flexible brain tissue. This raises question whether we need bridge this gap; would increasing flexibility proportionally FBR?Using novel off-stoichiometry thiol-enes-epoxy (OSTE+) polymer developed in our previous work,...
How do fungi navigate through the complex microscopic maze-like structures found in soil? Fungal behaviour, especially at hyphal scale, is largely unknown and challenging to study natural habitats such as opaque soil matrix. We monitored growth behaviour strategies of seven Basidiomycete litter decomposing species a micro-fabricated "Soil Chip" system that simulates principal aspects pore space its micro-spatial heterogeneity. The hyphae were faced with micrometre constrictions, sharp turns...
Gene expression has a strong stochastic element resulting in highly variable mRNA levels between individual cells, even seemingly homogeneous cell population. Access to fundamental information about cellular mechanisms, such as correlated gene expression, motivates measurements of multiple genes cells. Quantitative reverse transcription PCR (RT-qPCR) is the most accessible method which provides sufficiently accurate single Low concentration guanidine thiocyanate was used fully lyse...
The neuronal soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex is essential for synaptic vesicle exocytosis, but its study has been limited by the neonatal lethality of murine SNARE knockouts. Here, we describe a viable mouse line carrying mutation in b-isoform synaptosomal-associated 25 kDa (SNAP-25). causative I67T missense results increased binding affinities within complex, impaired exocytotic recycling and granule exocytosis pancreatic beta-cells,...
Key points α‐ and β‐cells express both Na v 1.3 1.7 + channels but in different relative amounts. The differential expression explains the properties of currents β‐cells. is functionally important channel α subunit Islet are locked an inactive state due to islet cell‐specific factor. Mouse pancreatic β‐ α‐cells equipped with voltage‐gated that inactivate over widely membrane potentials (half‐maximal inactivation ( V 0.5 ) at −100 mV −50 α‐cells, respectively). Single‐cell PCR analyses show...
The minor groove binding asymmetric cyanine dye 4-[(3-methyl-6-(benzothiazol-2-yl)-2,3-dihydro- (benzo-1,3-thiazole)-2-methylidene)]-1-methyl-pyridin ium iodide (BEBO) is tested as sequence non- specific label in real-time PCR. fluorescence intensity of BEBO increases upon to double-stranded DNA allowing emission be measured at the end elongation phase PCR cycle. concentrations between 0.1 and 0.4 micro M generated sufficient signal without inhibiting A comparison with commonly used reporter...
Mouse beta-cells cultured at 15 mmol/l glucose for 72 h had reduced ATP-sensitive K+ (K(ATP)) channel activity (-30%), increased voltage-gated Ca2+ currents, higher intracellular free concentration ([Ca2+]i; +160%), more exocytosis (monitored by capacitance measurements, +100%), and greater insulin content (+230%) than those 4.5 glucose. However, they released 20% less when challenged with 20 Glucose-induced (20 mmol/l) secretion was 60-90% in islets cocultured or either oleate palmitate...
OBJECTIVE The aim of the study was to elucidate cellular mechanism underlying suppression glucose-induced insulin secretion in mice fed a high-fat diet (HFD) for 15 weeks. RESEARCH DESIGN AND METHODS C57BL6J were HFD or normal (ND) 3 Plasma and glucose levels vivo assessed by intraperitoneal tolerance test. Insulin vitro studied using static incubations perfused pancreas preparation. Membrane currents, electrical activity, exocytosis examined patch-clamp technique measurements. Intracellular...
The presented "spot-on-a-chip" technology enables easy enrichment of samples in the low nanomolar (1−5 nM) range and provides a fast reliable automated sample preparation method for performing matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis with high sensitivity throughput. Through microdispensing, which allows accurate deposition 60-pL droplets, dilute were enriched by making multiple droplet depositions nanovials. was confined to defined...
In search of therapeutic agents for estrogen-related pathologies, phytoestrogens are being extensively explored. contrast to naringenin, 8-prenylnaringenin is a potent hop-derived estrogenic compound, highlighting the importance prenyl group hormonal activity. We investigated effects substituting at C(8) with alkyl chains varying lengths and branching patterns on estrogen receptor (ER) subtype ERalpha- ERbeta-binding affinities transcriptional activities. addition, features ligand-induced...