A5016902862- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- Metal-Catalyzed Oxygenation Mechanisms
- Bacterial Identification and Susceptibility Testing
- Ion-surface interactions and analysis
- Streptococcal Infections and Treatments
- Microfluidic and Bio-sensing Technologies
- Medical Imaging Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Advanced Biosensing Techniques and Applications
- Social and Educational Sciences
- Medical Image Segmentation Techniques
- Advanced biosensing and bioanalysis techniques
- Silicon Nanostructures and Photoluminescence
- SARS-CoV-2 and COVID-19 Research
- Museums and Cultural Heritage
- Infective Endocarditis Diagnosis and Management
- Analytical chemistry methods development
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Nanowire Synthesis and Applications
- Amyloidosis: Diagnosis, Treatment, Outcomes
- ATP Synthase and ATPases Research
Mid Sweden University
2025
Lund University
2015-2024
Stockholm University
1970-2024
Science for Life Laboratory
2023-2024
Uppsala University
2017-2022
Antaros Medical (Sweden)
2021
Luleå University of Technology
2014
Medicon Village
2013
AstraZeneca (Sweden)
1999-2000
Abstract Transfer RNA-derived fragments (tRFs) are emerging small noncoding RNAs that, although commonly altered in cancer, have poorly defined roles tumorigenesis 1 . Here we show that pseudouridylation (Ψ) of a stem cell-enriched tRF subtype 2 , mini tRFs containing 5′ terminal oligoguanine (mTOG), selectively inhibits aberrant protein synthesis programmes, thereby promoting engraftment and differentiation haematopoietic progenitor cells (HSPCs) patients with myelodysplastic syndrome...
Protein identification through peptide mass mapping by matrix-assisted laser desorption/ionization time-of-flight spectrometry (MALDI-TOF MS) has become a standard technique, used in many laboratories around the world. The traditional methodology often includes long incubations (6−24 h) and extensive manual steps. In an effort to address this, integrated microanalytical platform been developed for automated of proteins. silicon micromachined analytical tools, i.e., microchip immobilized...
This work presents a simple method for obtaining homogeneous sample surfaces in matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) the automated analysis of peptides and proteins. The preparation is based on applying sample/matrix mixture onto pre-deposited highly diluted matrix spot. crystals act as seeds new containing which become much smaller size more evenly distributed than with conventional methods. 'seed-layer' was developed, optimised compared...
Conventional approaches to isolate and characterize nanobodies are laborious. We combine phage display, multivariate enrichment, next-generation sequencing, a streamlined screening strategy identify numerous anti–severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nanobodies. their potency specificity using neutralization assays hydrogen/deuterium exchange mass spectrometry (HDX-MS). The most potent bind the receptor binding motif of domain (RBD), we two exceptionally members this...
The present work demonstrates the possibilities of using macroporous silicon as a substrate for highly sensitive protein chip applications. formation 3D porous structures was performed by electrochemical dissolution monocrystalline silicon. fabricated network has rigid spongelike structure showing high uniformity and mechanical stability. microfluidic properties substrates were found to be essential good bioassay performance. Small spot area, reproducibility, homogeneous profiles...
Amino acid starvation is sensed by Escherichia coli RelA and Bacillus subtilis Rel through monitoring the aminoacylation status of ribosomal A-site tRNA. These enzymes are positively regulated their product-the alarmone nucleotide (p)ppGpp-through an unknown mechanism. The (p)ppGpp-synthetic activity Rel/RelA controlled via auto-inhibition hydrolase/pseudo-hydrolase (HD/pseudo-HD) domain within enzymatic N-terminal region (NTD). We localize allosteric pppGpp site to interface between SYNTH...
The human immunoglobulin heavy-chain (IGH) locus is exceptionally polymorphic, with high levels of allelic and structural variation. Thus, germline IGH genotypes are personal, which may influence responses to infection vaccination. For an improved understanding inter-individual differences in antibody responses, we isolated SARS-CoV-2 spike-specific monoclonal antibodies from convalescent health care workers, focusing on the IGHV1-69 gene, has highest level variation all IGHV genes.
Abstract This study showcases an integrative mass spectrometry-based strategy combining systems antigenomics and serology to characterize human antibodies in clinical samples. involves using circulating plasma affinity-enrich antigenic proteins biochemically fractionated pools of bacterial proteins, followed by their identification quantification spectrometry. A selected subset the identified antigens is then expressed recombinantly isolate antigen-specific IgG, characterization structural...
Abstract Mammalian mitochondrial DNA (mtDNA) is replicated by polymerase γ (POLγ), a heterotrimeric complex consisting of catalytic POLγA subunit and two accessory POLγB subunits 1 . More than 300 mutations in POLG , the gene encoding subunit, have been linked to severe, progressive conditions with high rates morbidity mortality, for which no treatment exists 2 Here we report on discovery characterization PZL-A, first-in-class small-molecule activator mtDNA synthesis that capable restoring...
The presented "spot-on-a-chip" technology enables easy enrichment of samples in the low nanomolar (1−5 nM) range and provides a fast reliable automated sample preparation method for performing matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis with high sensitivity throughput. Through microdispensing, which allows accurate deposition 60-pL droplets, dilute were enriched by making multiple droplet depositions nanovials. was confined to defined...
With the human genome in a first sequence draft and several other genomes being finished this year, existing information gap between genomics proteomics is becoming increasingly evident. The analysis of proteome is, however, much more complicated because synthesis structural requirements functional proteins are different from easily handled oligonucleotides, for which analytical breakthrough already has come use DNA chips. In comparison with microarrays, protein arrays, or chips, offer...
Highly efficient washing and extraction of microbeads to decomplex analytes ranging from small peptides large viruses was realised in a microscaled continuous flow format. The bead principle reported herein is based on acoustophoresis, i.e. the primary acoustic radiation force an ultrasonic standing wave laminar properties are utilised translate bioanalytes trapped functionalised one carrier fluid another. carry-over non-specific material ranges 1 50 ppm relative input levels depending...
Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is currently changing the clinical routine for identification of microbial pathogens. One important application rapid bacteria diagnosis bloodstream infections (BSI). A novel approach based on acoustic trapping and an integrated selective enrichment target (ISET) microchip that improves sample preparation step this type analysis presented. The method evaluated clinically relevant samples in form...
This work describes the preparation and investigation of molecularly imprinted polymer (MIP) microgel (MG) stabilized Pickering emulsions (PEs) for their ability to catalyze formation disulfide bonds in peptides at O/W interface. The MIP MGs were synthesized via precipitation polymerization a programmed initiator change strategy. characterized using DLS analysis, SEM measurement, optical microscopy analysis. dry wet showed hydrodynamic diameter 100 280 nm, respectively. A template rebinding...
An important element of antibody-guided vaccine design is the use neutralizing or opsonic monoclonal antibodies to define protective epitopes in their native three-dimensional conformation. Here, we demonstrate a multimodal mass spectrometry-based strategy for in-depth characterization antigen-antibody complexes enable identification using cytolytic exotoxin Streptolysin O (SLO) from
Despite the high sensitivity and relatively tolerance for contaminants of matrix-assisted laser desorption/ionization-time flight mass spectrometry (MALDI-TOF MS) there is often a need to purify concentrate sample solution, especially after in-gel digestion proteins separated by two-dimensional gel electrophoresis (2-DE). A silicon microextraction chip (SMEC) clean-up trace enrichment peptides was manufactured investigated. The microchip structure used trap reversed-phase chromatography...
A microfabricated proteomic sample preparation and presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each nanovial can be filled with solid-phase extraction media for purification concentration peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The validity the ISET shown by analysis low nM-pM standard samples, as well biological samples. was compared ZipTip MassPREP...
A new read-out method for antibody arrays using laser desorption/ionization-mass spectrometry (LDI-MS) is presented. Small, photocleavable reporter molecules with a defined mass called "mass-tags" are used detection of immunocaptured proteins from human plasma. Using prostate specific antigen (PSA), biomarker cancer, as model antigen, high sensitivity generic methodology based immunocapture primary and biotin labeled secondary coupled to mass-tagged avidin demonstrated. As each can bind...
A recently introduced silicon microextraction chip (SMEC), used for on-line proteomic sample preparation, has proved to facilitate the process of protein identification by clean up and enrichment peptides. It is demonstrated that a novel grid-SMEC design improves operating characteristics solid-phase microextraction, reducing dispersion effects thereby improving preparation conditions. The structures investigated in this paper are treated both numerically experimentally. numerical approach...
Abstract The performance of a miniaturized sample processing platform for matrix‐assisted laser desorption/ionization‐mass spectrometry (MALDI‐MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in biological context. ISET serves as both treatment device and MALDI‐MS target, contains an array 96 perforated nanovials, which each can be filled with 40 nL reversed‐phase beads. This methodology minimizes the number...
This paper presents a new strategy to combine the power of antibody based capturing target species in complex samples with benefits microfluidic reverse phase sample preparation on an integrated enrichment (RP-ISET) and analysis speed MALDI MS. The immunoaffinity step is performed in-house developed 3D-structured high surface area porous silicon (PSi) matrix, which allows efficient immobilization by adsorption without any coupling agents 30–60 min. hydrophilic nature at molecular level...
With the rapid progress in development of new clinical biomarkers there is an unmet need fast and sensitive multiplex analysis methods for disease specific protein monitoring. Immunoaffinity extraction integrated with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) offers a route to potentially biomarker analysis. In this study, previously reported selective enrichment target (ISET)-MALDI-MS was implemented ssDNA aptamer functionalized microbeads address capturing...
Streptococcus pyogenes is known to cause both mucosal and systemic infections in humans. In this study, we used a combination of quantitative structural mass spectrometry techniques determine the composition structure interaction network formed between human plasma proteins surfaces different S. serotypes. Quantitative analysis revealed that forms serotype-specific networks are highly dependent on domain arrangement surface-attached M protein. Subsequent computational modeling one proteins,...
Abstract Phosphorylation is a protein post‐translational modification (PTM) that plays an important role in cell signaling, differentiation, and metabolism. The hyper phosphorylated forms of certain proteins have been appointed as biomarkers for neurodegenerative diseases, phosphorylation‐related mutations are detecting cancer pathways. Due to the low abundance biological fluids, sample enrichment beneficial prior detection. Thus, need find new strategies enriching phosphopeptides has...