A5030083039- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Escherichia coli research studies
- Yersinia bacterium, plague, ectoparasites research
- Bartonella species infections research
- Antibiotic Resistance in Bacteria
- Lipid Membrane Structure and Behavior
- Vector-borne infectious diseases
- Bacillus and Francisella bacterial research
- Genomics and Phylogenetic Studies
- Antimicrobial Peptides and Activities
- Bacterial biofilms and quorum sensing
- Rabies epidemiology and control
- Protein Structure and Dynamics
- Photosynthetic Processes and Mechanisms
- Vibrio bacteria research studies
- Mass Spectrometry Techniques and Applications
- Biochemical and Structural Characterization
- Viral Infections and Vectors
- Biosensors and Analytical Detection
- Toxin Mechanisms and Immunotoxins
- Protein purification and stability
- Advanced NMR Techniques and Applications
- Nanoparticles: synthesis and applications
University of Oslo
2016-2025
Czech Academy of Sciences, Institute of Microbiology
2022
Max Planck Institute for Developmental Biology
2009-2019
Max Planck Society
2006-2015
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
2015
Center for Integrated Protein Science Munich
2012
Technical University of Munich
2012
Ludwig-Maximilians-Universität München
2009
In-Q-Tel
2009
Technische Universität Berlin
2000-2004
Photosynthetic membranes of higher plants contain specific nonphosphorous lipids like the sulfolipid sulfoquinovosyl diacylglycerol in addition to ubiquitous phospholipid phosphatidylglycerol. In bacteria, an environmental factor that drastically affects thylakoid lipid composition appears be availability phosphate. Accordingly, we discovered increase relative amount and a concomitant decrease phosphatidylglycerol Arabidopsis thaliana grown on medium with reduced amounts phosphate, as well...
The spread of Gram-negative bacteria with plasmid-borne extended-spectrum beta-lactamases (ESBLs) has become a worldwide problem. This study analysed total 366 ESBL-producing Enterobacteriaceae strains isolated from non-selected patient specimens at the university hospital Tübingen in period January 2003 to December 2007. Although overall ESBL rate was comparatively low (1.6 %), percentages Enterobacter spp. and Escherichia coli increased 0.8 0.5 %, respectively, 4.6 3.8 % In particular,...
Proteomics studies of pathogenic bacteria are an important basis for biomarker discovery and the development antimicrobial drugs vaccines. Especially where vaccines concerned, it is great interest to explore which bacterial factors exposed on cell surface thus can be directly accessed by immune system. One crucial step in proteomics efficient subfractionation their cellular compartments. We set out compare improve different protocols fractionation proteins from Gram-negative into outer...
Abstract The tendency of bacteria to assemble at oil–water interfaces can be utilized create microbial recognition sites on the surface polymer beads. In this work, two different groups were first treated with acryloyl‐functionalized chitosan and then used stabilize an oil‐in‐water emulsion composed cross‐linking monomers that dispersed in aqueous buffer. Polymerization oil phase followed by removal bacterial template resulted well‐defined beads bearing imprints. Chemical passivation cell...
Metal nanoparticles have attracted considerable attention due to their astounding potential for a wide range of commercial applications. From targeted drug delivery and antimicrobial agents electronics, metal seem immeasurable prospects in all areas science. However, modern industrial production frequently involves complex procedures, large amounts energy, utilizes strong chemical solvents, or produces hazardous waste. Biological synthesis has been proposed as an alternative simpler,...
Extraction of membrane proteins from biological membranes is usually accomplished with the help detergents. This unit describes use detergents to solubilize and purify proteins. The chemical physical properties different classes typically used samples are discussed. A separate section addresses compatibility applications downstream protein purification process, such as optical spectroscopy, mass spectrometry, crystallography, or biomolecular NMR. Protocols in this include isolation...
Dermcidin encodes the anionic amphiphilic peptide DCD-1L, which displays a broad spectrum of antimicrobial activity under conditions resembling those in human sweat. Here, we have investigated its mode activity. We found that DCD-1L interacts preferentially with negatively charged bacterial phospholipids helix axis is aligned flat on lipid bilayer surface. Upon interaction bilayers forms oligomeric complexes are stabilized by Zn2+. able to form ion channels membrane, and propose Zn2+-induced...
Outer membrane β-barrels (OMBBs) are the major class of outer proteins from Gram-negative bacteria, mitochondria, and plastids. Their transmembrane domains consist 8–24 β-strands forming a closed, barrel-shaped β-sheet around central pore. Despite their obvious structural regularity, evidence for an origin by duplication or common ancestry has not been found. We use three complementary approaches to show that all OMBBs bacteria evolved single, ancestral ββ hairpin. First, we link almost...
Outer membrane proteins (OMPs) are the transmembrane found in outer membranes of Gram-negative bacteria, mitochondria and plastids. Most prediction methods have focused on analogous features, such as alternating hydrophobicity patterns. Here, we start from observation that almost all β-barrel OMPs related by common ancestry. We identify detecting their homologous relationships to known using sequence similarity. Given an input sequence, HHomp builds a profile hidden Markov model (HMM)...
Acinetobacter baumannii is a Gram-negative pathogen that causes multitude of nosocomial infections. The trimeric autotransporter adhesin (Ata) belongs to the superfamily adhesins which are important virulence factors in many species. Phylogenetic profiling revealed ata present 78% all sequenced A. isolates but only 2% closely related species calcoaceticus and pittii. Employing markerless deletion mutant ATCC 19606 we show adhesion invasion into human endothelial epithelial cells depend on...