A5039580254- Hemoglobin structure and function
- Protein purification and stability
- Heme Oxygenase-1 and Carbon Monoxide
- Monoclonal and Polyclonal Antibodies Research
- Microfluidic and Capillary Electrophoresis Applications
- Neonatal Health and Biochemistry
- Analytical Chemistry and Chromatography
- Plant tissue culture and regeneration
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Photosynthetic Processes and Mechanisms
- Protein Structure and Dynamics
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Bacterial Genetics and Biotechnology
- Analytical chemistry methods development
- Biochemical and Molecular Research
- Advanced biosensing and bioanalysis techniques
- Transgenic Plants and Applications
- Chemical Synthesis and Analysis
- CRISPR and Genetic Engineering
- Chemical and Physical Properties in Aqueous Solutions
- Pancreatic function and diabetes
Lund University
2015-2024
University Hospital Cologne
2017
University of Cologne
2017
John Wiley & Sons (United States)
2015
International Union for Pure and Applied Biophysics
1996-2013
Getinge (Sweden)
2013
Novo Nordisk (Denmark)
2008
Ideon Science Park
2008
Swedish e-Science Research Centre
1998-2001
ETH Zurich
1988
The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of yeast PGK1 promoter. recombinant showed highest activity at 85 degrees C with a specific 1.0 U mg-1. A new functional metabolic pathway S. ethanol formation during oxygen-limited fermentation demonstrated. Xylitol acetic acid were also formed fermentation.
Abstract The tendency of bacteria to assemble at oil–water interfaces can be utilized create microbial recognition sites on the surface polymer beads. In this work, two different groups were first treated with acryloyl‐functionalized chitosan and then used stabilize an oil‐in‐water emulsion composed cross‐linking monomers that dispersed in aqueous buffer. Polymerization oil phase followed by removal bacterial template resulted well‐defined beads bearing imprints. Chemical passivation cell...
Aim: Hemoglobin (Hb) becomes toxic when released from the erythrocyte.The acute phase protein haptoglobin (Hp) binds avidly to Hb and decreases oxidative damage itself surrounding proteins lipids.However, molecular mechanism underpinning Hp protection is date unclear.The aim of this study was use electron paramagnetic resonance (EPR) spectroscopy, stopped flow optical spectrophotometry, sitedirected mutagenesis explore specifically role specific tyrosine residues in protection.Results:...
Aim: Hemoglobin (Hb) becomes toxic when released from the erythrocyte.The acute phase protein haptoglobin (Hp) binds avidly to Hb and decreases oxidative damage itself surrounding proteins lipids.However, molecular mechanism underpinning Hp protection is date unclear.The aim of this study was use electron paramagnetic resonance (EPR) spectroscopy, stopped flow optical spectrophotometry, sitedirected mutagenesis explore specifically role specific tyrosine residues in protection.Results:...
We present a new method for preparation of protein-specific polymer beads based on surface molecular imprinting in Pickering emulsion. In the first step, adult human hemoglobin (Hb) was adsorbed silica nanoparticles. The protein-coated particles were then used to stabilize an oil-in-water emulsion (Pickering emulsion) composed cross-linking monomer oil phase. After free radical polymerization phase, protein-silica removed leave Hb-imprinted sites surface. protein-imprinted microspheres...
In this study, galactose dehydrogenase (EC 1.1.1.48) was chosen as a prototype target protein to investigate the capability of metal affinity precipitation facilitate purification genetically engineered proteins. A DNA fragment encoding five histidine residues fused 3′‐terminal end gene from Pseudomonas fluorescens and thereafter expressed in Escherichia coli . The additional histidines functioned an tail modified enzyme could be purified using when metal‐chelate complex with ethylene...
Oat (
In searching for novel peptides with affinity cadmium, the phage display technique was utilized. selection procedure, cadmium ions were immobilized on a metal chelating Sepharose gel. The selected from hexapeptide library showed no homology to naturally occurring metallothioneins. From clones in biopanning process, phages Cd-109 free solution identified. peptide His-Ser-Gln-Lys-Val-Phe, which found exhibit strongest relative Cd-109, cloned into Escherichia coli as fusion cell surface exposed...
l-Lactate dehydrogenase (LDH) from Bacillus stearothermophilus is a redox enzyme which has strong preference for NADH over NADPH as coenzyme. To exclude the coenzyme-binding pocket, LDH contains conserved aspartate residue at position 52. However, this probably not solely responsible specificity. In report we examine possibilities of altering coenzyme specificity by introducing range different point mutations in domain. Furthermore, after choosing mutant with highest selectivity NADPH, also...
Cereals accumulate starch in the endosperm as their major energy reserve grain. In most cereals embryo, scutellum, and aleurone layer are high oil, but these tissues constitute a very small part of total seed weight. However, oat (Avena sativa L.) oil kernels is deposited same cells that starch. Thus desirable model system to study metabolic switches responsible for carbon partitioning between synthesis. A prerequisite such investigations development an experimental allows flux analysis...
• Premise of the study : Storage oil (triacylglycerol) accumulates in tissues such as embryo and endosperm seeds fruit mesocarp, but seldom underground organs. As a rare exception, cultivated variants yellow nutsedge ( Cyperus esculentus ) contain high amounts both starch mature tubers. Methods Biochemical analyses light electron microscopy were used to accumulation patterns storage nutrients developing Key results During initial phase tuber development, conducting rhizome tissue is...