A5084935205- CRISPR and Genetic Engineering
- Potato Plant Research
- Food composition and properties
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant Pathogens and Resistance
- Plant nutrient uptake and metabolism
- Microbial Metabolites in Food Biotechnology
- Lipid metabolism and biosynthesis
- Plant Disease Resistance and Genetics
- Nanocomposite Films for Food Packaging
- Phytase and its Applications
- Biofuel production and bioconversion
- Advanced Breast Cancer Therapies
- Breast Cancer Treatment Studies
- Oil Palm Production and Sustainability
- Transgenic Plants and Applications
- Virus-based gene therapy research
- Research in Cotton Cultivation
- biodegradable polymer synthesis and properties
- Plant biochemistry and biosynthesis
- RNA Interference and Gene Delivery
- Genetically Modified Organisms Research
- Estrogen and related hormone effects
Swedish University of Agricultural Sciences
2014-2024
Norwegian University of Life Sciences
2023
John Innes Centre
2023
Rigshospitalet
2021
Lund University
2004-2009
International Union for Pure and Applied Biophysics
2004
Danish Meat Trade College
1999
Technical University of Denmark
1998
Clustered regularly interspaced short palindromic repeats and CRISPR‐associated protein‐9 (CRISPR‐Cas9) can be used as an efficient tool for genome editing in potato ( Solanum tuberosum ). From both a scientific regulatory perspective, it is beneficial if integration of DNA the avoided. We have implemented DNA‐free method, using delivery CRISPR‐Cas9 ribonucleoproteins (RNPs) to protoplasts, by targeting gene encoding granule bound starch synthase (GBSS, EC 2.4.1.242). The RNP method was...
Adjuvant abemaciclib combined with endocrine therapy (ET) previously demonstrated clinically meaningful improvement in invasive disease-free survival (IDFS) and distant relapse-free (DRFS) hormone receptor-positive, human epidermal growth factor receptor 2-negative, node-positive, high-risk early breast cancer at the second interim analysis, however follow-up was limited. Here, we present results of prespecified primary outcome analysis an additional analysis.This global, phase III,...
Oil palm (Elaeis guineensis) produces two oils of major economic importance, commonly referred to as oil and kernel oil, extracted from the mesocarp endosperm, respectively. While lauric acid predominates in endosperm fatty acids (FAs) are palmitic oleic acids. The embryo also stores which contains a significant proportion linoleic acid. In addition, three tissues display high variation for content at maturity. To gain insight into mechanisms that govern such differences FA composition,...
Polyphenol Oxidases (PPOs) catalyze the conversion of phenolic substrates to quinones, leading formation dark-colored precipitates in fruits and vegetables. This process, known as enzymatic browning, is cause undesirable changes organoleptic properties loss nutritional quality plant-derived products. In potato (Solanum tubersoum L.), PPOs are encoded by a multi-gene family with different expression patterns. Here, we have studied application CRISPR/Cas9 system induce mutations StPPO2 gene...
We show that human adenovirus inhibits RNA interference (RNAi) at late times of infection by suppressing the activity two key enzyme systems involved, Dicer and RNA-induced silencing complex (RISC). To define mechanisms which blocks RNAi, we used a panel mutant adenoviruses defective in virus-associated (VA) expression. The results RNAs, VA RNAI RNAII, function as suppressors RNAi interfering with Dicer. RNAs bind competitive substrates squelching Further, RNAII are processed Dicer, both...
Abstract DNA-free genome editing was used to induce mutations in one or two branching enzyme genes ( Sbe ) tetraploid potato develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein (RNP) transfection of protoplasts, a mutation frequency up 72% achieved. The large variation grouped as follows: Group 1 lines all alleles Sbe1 mutated, 2 well three Sbe2 mutated 3 having both mutated. Starch from found be essentially free no detectable chain...
Tubers from a genetically modified high-amylose line T-2012 and its parental potato cultivar Dinamo were analyzed for resistant starch (RS) dietary fiber (DF) after cooking cold storage. For uncooked potatoes, the tubers (30% of dry matter, DM) had much lower RS than parent (56% DM). However, cooking, gave more (13% (4% DM), level increased further to about 20% DM 1 day The altered content was attributable changes in amylose content, granule structure, amylopectin structure induced by...
Summary Tuber and root crops virtually exclusively accumulate storage products in the form of carbohydrates. An exception is yellow nutsedge ( Cyperus esculentus ) which tubers have capacity to store starch triacylglycerols TAG roughly equal amounts. This suggests that a tuber crop can efficiently handle accumulation energy dense oil. From nutritional as well economic aspect, it would be interest utilize high yield or for oil similar nutsedge. The transcription factor WRINKLED 1 from...
We have established a DNA-free genome editing method via ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant plants regenerated from transfected protoplasts with high mutation rate. The application of as research breeding has provided many possibilities to improve traits crops recent years. In (Solanum lycopersicum), so far only stable Agrobacterium-mediated transformation carrying reagents been established. Shoot regeneration is the major bottleneck tomato. this...
Seed oils of many Cuphea sp. contain >90% medium-chain fatty acids, such as decanoic acid (10:0). These seed oils, which are among the most compositionally variant in plant kingdom, arise from specialized biosynthetic enzymes and acyltransferases. include lysophosphatidic acyltransferases (LPAT) diacylglycerol (DGAT) that required for successive acylation acids sn-2 sn-3 positions triacylglycerols (TAGs). Here we report identification a cDNA DGAT1-type enzyme, designated CpuDGAT1,...
Potato starch with mutations in branching enzyme genes (SBEI, SBEII) and granule-bound synthase gene (GBSS) was characterized for molecular thermal properties. Mutations GBSS were here stacked to a previously developed SBEI SBEII mutation line. Additionally, the alone induced wild-type variety comparison. The parental line SBE showed ∼ 40 % increase amylose content compared wild-type. GBSS-SBEI-SBEII produced non-waxy, low-amylose lines An exception one remaining allele, which displayed ∼80...
Potato is an important crop for starch production, but there are limitations regarding the genetic variation of quality. In maize, starches with various properties have been available a long time by mutational breeding. Amylose from potatoes differs cereal amyloses in several functionally aspects, such as higher degree polymerization. Areas application which polymerization importance include film forming and polymeric bioplastics. High-amylose potato lines achieved inhibiting two known...
To cope with cold stress, plants have developed antioxidation strategies combined osmoprotection by sugars. In potato (Solanum tuberosum) tubers, which are swollen stems, exposure to stress induces starch degradation and sucrose synthesis. Vacuolar acid invertase (VInv) activity is a significant part of the cold-induced sweetening (CIS) response, rapidly cleaving into hexoses increasing osmoprotection. discover alternative plant tissue pathways for coping we produced VInv-knockout lines in...
Lysophosphatidic acid acyltransferase (LPAT) catalyzes acylation of the sn-2 position on lysophosphatidic by an acyl CoA substrate to produce phosphatidic precursor polar glycerolipids and triacylglycerols (TAGs). In case TAGs, this reaction is typically catalyzed LPAT2 from microsomal LPAT class A that has high specificity for C18 fatty acids containing Δ9 unsaturation. Because specificity, occurrence saturated in TAG infrequent seed oils. To identify LPATs with variant specificities, deep...
The correlation between starch internal structure and thermal properties (gelatinisation retrogradation) was studied in starches of wild-type potatoes from the lines with altered synthase or branching enzyme activities (GBSS, SS, SBE), representing a range amylose:amylopectin ratio 2 to >99.5% amylopectin. different potato were divided into Group 1–8 depending on mutation targets. chain distribution building block composition beta-limit dextrins (β-LDs) whole analysed by high-performance...
Starch is the predominant storage compound in underground plant tissues like roots and tubers. An exception sugar beet tap-root (Beta vulgaris ssp altissima) which exclusively stores sucrose. The underlying mechanism behind this divergent accumulation currently not fully known. From general presence of starch tubers it could be speculated that lack tap-roots would originate from deficiency pathways leading to starch. Therefore with emphasis on accumulation, we studied using parsnip...
In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical gas barrier properties using extrusion. Characterization MPS revealed an altered chain length distribution amylopectin fraction slightly increased amylose content compared wild type starch. WG different ratios plasticized either glycerol or water extruded at 110 130 °C. The nanomorphology showed having semicrystalline structure...