A5030690898- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Plant Parasitism and Resistance
- CRISPR and Genetic Engineering
- Plant Pathogens and Resistance
- Plant Pathogenic Bacteria Studies
- Plant Molecular Biology Research
- Plant Disease Resistance and Genetics
- Cocoa and Sweet Potato Agronomy
- Chromosomal and Genetic Variations
- Plant Pathogens and Fungal Diseases
- Insect symbiosis and bacterial influences
- Legume Nitrogen Fixing Symbiosis
- Advances in Cucurbitaceae Research
- Powdery Mildew Fungal Diseases
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Toxin Mechanisms and Immunotoxins
- Bacteriophages and microbial interactions
- Potato Plant Research
Agricultural Research Organization
2013-2022
National Agricultural Research Organisation
1994-2021
GTx (United States)
2011
Ceragon (Israel)
2006
Weizmann Institute of Science
2006
Cornell University
1994-1997
Noble Research Institute
1994
Summary Genome editing in plants has been boosted tremendously by the development of CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) technology. This powerful tool allows substantial improvement plant traits addition to those provided classical breeding. Here, we demonstrate virus resistance cucumber ( Cucumis sativus L.) using Cas9/subgenomic RNA (sgRNA) technology disrupt function recessive eIF4E eukaryotic translation initiation factor 4E ) gene. Cas9/sgRNA...
Chlorophyll is a central player in harvesting light energy for photosynthesis, yet the rate-limiting steps of chlorophyll catabolism and regulation catabolic enzymes remain unresolved. To study role chlorophyllase (Chlase), first enzyme pathway, we expressed precursor mature versions citrus (Citrus sinensis) Chlase two heterologous plant systems: (1) squash (Cucurbita pepo) plants using viral vector expression system; (2) transiently transformed tobacco (Nicotiana tabacum) protoplasts....
The helper component-proteinase (HC-Pro) protein of potyviruses is a suppressor gene silencing and has been shown to elicit plant developmental-defect-like symptoms. In Zucchini yellow mosaic virus (ZYMV), mutation in the highly conserved FR180NK box HC-Pro FI180NK causes attenuation these At 5 days postinoculation before symptoms appear, accumulation, levels, viral short interfering RNA (siRNA) levels are similar for severe (FRNK) attenuated (FINK) strains. this stage, ZYMV(FRNK) caused...
Significance RNAi-mediated antiviral immunity directs specific virus resistance by virus-derived siRNAs in contrast to broad-spectrum triggered innate host pattern recognition receptors. Here we show that induction of RNAi Arabidopsis is associated with production a genetically distinct class virus-activated (vasiRNAs) RNA-dependent RNA polymerase-1 target hundreds genes for silencing Argonaute-2. Production vasiRNAs induced viruses from two different supergroups families, targeted...
Broomrapes (Phelipanche aegyptiaca and Orobanche spp.) are obligate plant parasites that cause extreme damage to crop plants. The parasite seeds have strict requirements for germination, involving preconditioning exposure specific chemicals strigolactones [SLs] exuded by the host roots. SLs hormones derived from carotenoids via a pathway Carotenoid Cleavage Dioxygenase 8 (CCD8). Having no effective means control parasitic weeds in most crops, with CRISPR/Cas9 being an gene-editing tool, here...
The genus Solanum comprises three food crops (potato, tomato, and eggplant), which are consumed on daily basis worldwide also producers of notorious anti-nutritional steroidal glycoalkaloids (SGAs). Hydroxylated SGAs (i.e. leptinines) serve as precursors for leptines that act defenses against Colorado Potato Beetle (Leptinotarsa decemlineata Say), an important pest potato worldwide. However, SGA hydroxylating enzymes remain unknown. Here, we discover 2-OXOGLUTARATE-DEPENDENT-DIOXYGENASE...
Abstract Shoot branching is an important aspect of plant architecture because it substantially affects biology and agricultural performance. Sugars play role in the induction shoot several species, including potato (Solanum tuberosum L.). However, mechanism by which sugars affect remains mostly unknown. In present study, we addressed this question using sugar-mediated bud outgrowth stems under etiolated conditions. Our results indicate that sucrose feeding to detached promotes accumulation...
Transmission of zucchini yellow mosaic virus (ZYMV) by aphids was examined introducing mutations within the highly conserved proline-threonine-lysine (PTK) motif helper component proteinase (HC-Pro) using a cDNA full-length clone. Replacement proline alanine (ATK) in PTK abolished transmission almost completely both from plants and membranes. Substitution basic lysine glutamic acid (PTE) did not reduce rate compared with wild- type. threonine valine (PVK) or serine (PSK) resulted that lower...
Sequence comparison had previously shown three amino acid changes in conserved motifs the 455-amino sequence of helper component-protease (HC-Pro) between a severe field strain Zucchini yellow mosaic virus (ZYMV-NAT) and mild ZYMV (ZYMV-WK). In this study, exchange fragments site-directed mutagenesis within HC-Pro gene an infectious clone enabled effects mutations on symptom expression to be mapped. The substitution Ile for Arg at position 180 motif Phe-Arg-Asn-Lys (FRNK) potyviruses was...
ABSTRACT Mixed infections of cucurbits by Cucumber mosaic virus (CMV) and potyviruses exhibit a synergistic interaction. Zucchini squash melon plants coinfected the potyvirus yellow (ZYMV) either Fny-CMV (subgroup IA) or LS-CMV II) displayed strong pathological responses, eventually progressing to vascular wilt plant death. Accumulation Fny- RNAs in mixed infection with ZYMV zucchini was slightly higher than CMV strains alone. There an increase (+) strand RNA levels, but no (-) RNA3 levels...
The differential rate of systemic symptom induction in zucchini squash by the Fny- and Sny-strains cucumber mosaic virus (CMV) previously was mapped to RNA 1, which encodes a protein (1a) involved replication. Examination kinetics accumulation RNAs four encoded proteins inoculated cotyledons showed that Fny-CMV-associated products generally appeared earlier than Sny-CMV-associated products. In systematically infected leaves, this difference magnified, with 2-day delay appearance Sny-CMV...
The nucleotide sequence of the helper component protease (HC-Pro) genes three zucchini yellow mosaic virus (ZYMV) strains has been compared with that a helper-deficient strain ZYMV-HC. comparisons revealed unique deduced amino acid differences. Two these mutations were located in regions which are conserved other potyviruses. role aphid transmissibility was examined by exchanging DNA fragments part deficient HC- Pro gene respective section within infectious full-length clone...
Abstract The fungal biocontrol agent Trichoderma asperellum has been recently shown to induce systemic resistance in plants through a mechanism that employs jasmonic acid and ethylene signal transduction pathways. Mitogen-activated protein kinase (MAPK) proteins have implicated the of wide variety plant stress responses. Here we report identification characterization Trichoderma-induced MAPK (TIPK) gene function cucumber (Cucumis sativus). Similar its homologs, wound-induced kinase, MPK3,...
Summary Orobanche spp. (broomrape) are parasitic plants which subsist on the roots of a wide range hosts, including tomato, causing severe losses in yield quality and quantity. Large amounts mannitol accumulate this weed during development. Mannose 6‐phosphate reductase (M6PR) is key enzyme biosynthesis, it has been suggested that accumulation may be very important for Therefore, M6PR gene potential target efforts to control parasite. Transgenic tomato were produced bearing construct...
The flavonoids are phenylpropanoid-derived metabolites that ubiquitous in plants, playing many roles growth and development. Recently, we observed fruit rinds of yellow casaba muskmelons (Cucumis melo 'Inodorous Group') accumulate naringenin chalcone, a flavonoid pigment. With RNA-sequencing analysis bulked segregants representing the tails population segregating for chalcone accumulation followed by fine mapping genetic transformation, identified Kelch domain-containing F-box protein coding...
During 2019, tomato fruits showing viral-like symptoms of marbled yellow spots were abundant in Israel. The new distinctive from those typical brown rugose fruit virus (ToBRFV) infection but resembled pepino mosaic (PepMV) infection. RT-PCR analysis and the serological tests (enzyme linked immunosorbent assay, western blot situ immunofluorescence) revealed confirmed presence both tobamovirus ToBRFV potexvirus PepMV symptomatic fruits. A mixture rod-like filamentous particles, characteristic...
An aphid-transmissible (AT) and two non-aphid-transmissible (NAT) isolates of zucchini yellow mosaic virus (ZYMV) were studied. The predicted amino acid sequences the coat protein (CP) three analysed compared. NAT differed from AT isolate in having a Thr instead an Ala residue at position 10 conserved Asp-Ala-Gly triplet N-terminal region CP. Aphid transmissibility was restored progeny derived infectious clone ZYMV-NAT which changed back to by site-directed mutagenesis. However this mutation...
SUMMARY Taxonomy: Zucchini yellow mosaic virus (ZYMV) is a member of genus Potyvirus , family Potyviridae. ZYMV positive‐strand RNA virus. Physical properties: Virions are flexuous filaments 680–730 nm in length and 11–13 diameter, composed about 2000 subunits single 31‐kDa protein (calculated). The genome size 9.6 kb covalently linked to viral‐encoded (the VPg) at the 5′ end, with 3′ poly A tail. end sequence AU‐rich (69%). Viral proteins: expressed as polyprotein cleaved by three viral...
SUMMARY More than 44 species of aphids were trapped by suction during the spring seasons 1981, 1982 and 1983 over a pepper field at Bet Dagan, Israel. Nineteen transmitted cucumber mosaic virus (CMV), while seven potato Y (PVY) least once. Over 80% CMV PVY infection among test plants ( Capsicum annuum cv. Weindale) exposed to was caused Aphis citricola two or three other species, Myzus persicae Macrosiphum euphorbiae. Landing rate determined comparing proportion each found on green tiles...
Gene-silencing has been used to develop resistance against many plant viruses but little is known about the transgenic small-interfering RNA (t-siRNA) that confers this resistance. Transgenic cucumber and melon lines harboring a hairpin construct of Zucchini yellow mosaic potyvirus (ZYMV) HC-Pro gene accumulated different levels t-siRNA (6 44% total siRNA) exhibited systemic ZYMV infection. Resistance Watermelon Papaya ring spot potyvirus-W was also observed in line high (44% displayed...
Abstract Virus-host coevolution often drives virus immune escape. However, it remains unknown whether natural variations of plant resistance are enriched in genes RNA interference (RNAi) pathway known to confer essential antiviral defense plants. Here, we report two genome-wide association study screens interrogate variation among wild-collected Arabidopsis thaliana accessions quantitative the endemic cucumber mosaic (CMV). We demonstrate that highest-ranked gene significantly associated...
ABSTRACT Zucchini yellow mosaic virus (ZYMV, Potyvirus) is a very damaging cucurbit worldwide. Interspecific crosses with resistant Cucurbita moschata have led to the release of "resistant" zucchini squash (C. pepo) F(1) hybrids. However, although resistance almost complete in C. moschata, commercial pepo hybrids are only tolerant. ZYMV evolution toward increased aggressiveness on tolerant was observed field and obtained experimentally. Sequence comparisons recombination experiments revealed...