A5001930617- Genetic Mapping and Diversity in Plants and Animals
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Genetics and Plant Breeding
- Plant Gene Expression Analysis
- Agricultural Practices and Plant Genetics
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant Pathogens and Resistance
- Antioxidant Activity and Oxidative Stress
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Postharvest Quality and Shelf Life Management
- Plant and animal studies
- Plant nutrient uptake and metabolism
- Plant Physiology and Cultivation Studies
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Potato Plant Research
- Genetic and Environmental Crop Studies
- Plant Parasitism and Resistance
Hebrew University of Jerusalem
2016-2025
Institute of Life Sciences
2024
Jerusalem University College
2018
St. Mary's College of Maryland
2015
Michigan State University
2015
Max Planck Institute of Molecular Plant Physiology
2004-2011
University of Florida
2010
Cornell University
1988-2006
University of Georgia
2006
Agricultural Research Organization
2002-2005
Abstract As part of ongoing studies regarding the genetic basis quantitative variation in phenotype, we have determined chromosomal locations trait loci (QTLs) affecting fruit size, soluble solids concentration, and pH, a cross between domestic tomato (Lycopersicon esculentum Mill.) closely-related wild species, L. cheesmanii. Using RFLP map genome, compared inheritance patterns polymorphisms 350 F2 individuals with phenotypes scored three different ways: (1) from progeny themselves, grown...
Modern commercial tomato varieties are substantially less flavorful than heirloom varieties. To understand and ultimately correct this deficiency, we quantified flavor-associated chemicals in 398 modern, heirloom, wild accessions. A subset of these accessions was evaluated consumer panels, identifying the that made most important contributions to flavor liking. We found modern contain significantly lower amounts many older Whole-genome sequencing a genome-wide association study permitted...
Carotenoid pigments in plants fulfill indispensable functions photosynthesis. Carotenoids that accumulate as secondary metabolites chromoplasts provide distinct coloration to flowers and fruits. In this work we investigated the genetic mechanisms regulate accumulation of carotenoids during ripening tomato We analyzed two mutations affect fruit pigmentation ( Lycopersicon esculentum ): Beta B ), a single dominant gene increases β-carotene fruit, old-gold og recessive mutation abolishes...
Carotenoid biosynthesis in plants has been described at the molecular level for most of biochemical steps pathway. However, cis-trans isomerization carotenoids, which is known to occur vivo, remained a mystery since its discovery five decades ago. To elucidate mechanism carotenoid isomerization, we have taken genetic map-based approach clone tangerine locus from tomato. Fruit are orange and accumulate prolycopene (7Z,9Z,7′Z,9′Z-tetra-cis-lycopene) instead all-trans-lycopene, normally...
Summary The red colour of tomato ( Lycopersicon esculentum ) fruits is provided by the carotenoid pigment lycopene whose concentration increases dramatically during ripening process. A single dominant gene, Del , in mutant Delta changes fruit to orange as a result accumulation δ‐carotene at expense lycopene. cDNA for ε‐cyclase CrtL‐e ), which converts δ‐carotene, was cloned from tomato. primary structure CRTL‐E 71% identical homologous polypeptide Arabidopsis and 36% β‐cyclase, CRTL‐B. gene...
To explore natural biodiversity we developed and examined introgression lines (ILs) containing chromosome segments of wild species (Solanum pennellii) in the background cultivated tomato (S. lycopersicum). We identified Brix9-2-5, which is a S. pennellii quantitative trait locus (QTL) that increases sugar yield tomatoes was mapped within flower- fruit-specific invertase (LIN5). QTL analysis representing five different delimited functional polymorphism Brix9-2-5 to an amino acid near...
Abstract The SOL Genomics Network (SGN; http://sgn.cornell.edu) is a rapidly evolving comparative resource for the plants of Solanaceae family, which includes important crop and model such as potato (Solanum tuberosum), eggplant melongena), pepper (Capsicum annuum), tomato lycopersicum). aim SGN to relate these species one another using genomics approach tie them other dicots through fully sequenced genome Arabidopsis (Arabidopsis thaliana). currently houses map marker data species, large...
Solanum pennellii is a wild tomato species endemic to Andean regions in South America, where it has evolved thrive arid habitats. Because of its extreme stress tolerance and unusual morphology, an important donor germplasm for the cultivated lycopersicum. Introgression lines (ILs) which large genomic S. lycopersicum are replaced with corresponding segments from can show remarkably superior agronomic performance. Here we describe high-quality genome assembly parents IL population. By...
In nature, genetic variation usually takes the form of a continuous phenotypic range rather than discrete classes. The underlying quantitative traits results from segregation numerous interacting trait loci (QTLs), whose expression is modified by environment. To uncover molecular basis this variation, we characterized QTL ( Brix9 - 2 5 ) derived green-fruited tomato species Lycopersicon pennellii . wild-species allele increased glucose and fructose contents in cultivated fruits various...
Natural biodiversity is an underexploited sustainable resource that can enrich the genetic basis of cultivated plants with novel alleles improve productivity and adaptation. We evaluated progress in breeding for increased tomato (Solanum lycopersicum) yield using genotypes carrying a pyramid three independent yield-promoting genomic regions introduced from drought-tolerant green-fruited wild species Solanum pennellii. Yield hybrids parented by pyramided was more than 50% higher control...
Summary Carotenoids are present in most tissues of higher plants where they play a variety essential roles. To study the regulation carotenoid biosynthesis, we have isolated novel mutations tomato ( Solanum lycopersicum ) with altered pigmentation fruit or flowers. Here describe isolation and analysis mutant, high‐pigment 3 hp3 ), that accumulates 30% more carotenoids mature fruit. Higher concentrations chlorophyll were also measured leaves pericarp green The mutation had occurred gene for...
Updates in nanopore technology have made it possible to obtain gigabases of sequence data. Prior this, sequencing was mainly used analyze microbial samples. Here, we describe the generation a comprehensive data set with median read length 11,979 bp for self-compatible accession wild tomato species Solanum pennellii We assembly its genome contig N50 2.5 MB. The pipeline comprised initial correction Canu and SMARTdenovo. resulting raw nanopore-based de novo is structurally highly similar that...
Abstract A large-scale metabolic quantitative trait loci (mQTL) analysis was performed on the well-characterized Solanum pennellii introgression lines to investigate genomic regions associated with secondary metabolism in tomato fruit pericarp. In total, 679 mQTLs were detected across 76 lines. Heritability analyses revealed that of less affected by environment than primary metabolism. Network allowed us assess interconnectivity and as well compare contrast their respective associations...