A5073438755- Metabolomics and Mass Spectrometry Studies
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Microbial Metabolic Engineering and Bioproduction
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Algal biology and biofuel production
- Legume Nitrogen Fixing Symbiosis
- Analytical Chemistry and Chromatography
- RNA and protein synthesis mechanisms
- Plant biochemistry and biosynthesis
- Plant responses to water stress
- Plant-Microbe Interactions and Immunity
- GABA and Rice Research
- Advanced Proteomics Techniques and Applications
- Advanced Chemical Sensor Technologies
- RNA modifications and cancer
- Horticultural and Viticultural Research
- Rice Cultivation and Yield Improvement
- Bioinformatics and Genomic Networks
- Plant Pathogens and Resistance
- Potato Plant Research
- Microbial Community Ecology and Physiology
- Mycorrhizal Fungi and Plant Interactions
Max Planck Institute of Molecular Plant Physiology
2016-2025
University of Córdoba
2016
University of British Columbia
2016
Max Planck Society
2006-2015
Leibniz Institute of Plant Biochemistry
2005-2014
University of Rostock
2008-2011
Bielefeld University
2011
Kansas State University
2011
Ludwig-Maximilians-Universität München
2011
Institut des Sciences des Plantes de Montpellier
2010
Summary: Metabolomics, in particular gas chromatography–mass spectrometry (GC–MS) based metabolite profiling of biological extracts, is rapidly becoming one the cornerstones functional genomics and systems biology. Metabolite has profound applications discovering mode action drugs or herbicides, unravelling effect altered gene expression on metabolism organism performance biotechnological applications. As such technology needs to be available many laboratories. For this, an open exchange...
A new method is presented in which gas chromatography coupled to mass spectrometry (GC-MS) allows the quantitative and qualitative detection of more than 150 compounds within a potato tuber, highly sensitive specific manner. In contrast other methods developed for metabolite analysis plant systems, this represents an unbiased open approach that unexpected changes levels. Although compromise wide range metabolites terms extraction, chemical modification GC-MS analysis, 25 analysed detail...
Metabolic profiling analyses were performed to determine metabolite temporal dynamics associated with the induction of acquired thermotolerance in response heat shock and freezing tolerance cold shock. Low-M(r) polar using gas chromatography-mass spectrometry. Eighty-one identified metabolites 416 unidentified mass spectral tags, characterized by retention time indices specific fragments, monitored. Cold influenced metabolism far more profoundly than The steady-state pool sizes 143 311 or...
Gas chromatography-mass spectrometry based metabolite profiling of biological samples is rapidly becoming one the cornerstones functional genomics and systems biology. Thus, technology needs to be available many laboratories open exchange information required such as those achieved for transcript protein data. The key-step in unambiguous identification metabolites highly complex preparations with composite structure. Collections mass spectra, which comprise frequently observed identified...
Unknown compounds in polar fractions of Arabidopsis thaliana crude leaf extracts were identified on the basis calculations elemental compositions obtained from gas chromatography/low-resolution quadrupole mass spectrometric data. Plant metabolites methoximated and silylated prior to analysis. All known peaks used as internal references construct polynomial recalibration curves raw Mass accuracies 0.005 ± 0.003 amu isotope ratio errors 0.5 0.3% (A + 1/A), respectively, 0.3 0.2% 2/A), could be...
Abstract Motivation: Typical GC-MS-based metabolite profiling experiments may comprise hundreds of chromatogram files, which each contain up to 1000 mass spectral tags (MSTs). MSTs are the characteristic patterns ∼25–250 fragment ions and respective isotopomers, generated after gas chromatography (GC) by electron impact ionization (EI) separated chemical molecules. These subsequently detected time-of-flight (TOF) spectrometry (MS). typically reported as a list ions, characterized mass,...
Exposure of Arabidopsis to low temperatures results in cold acclimation where freezing tolerance is enhanced. To achieve a wider view the role transcriptome biochemical changes that occur during acclimation, analyses concurrent transcript and metabolite was performed revealing dynamics selected gene-metabolite relationships. temperature resulted broad transcriptional responses. Principal component analysis revealed sequentially progressive, global both gene expression profiles acclimation....
Abstract Sulfur is an essential macroelement in plant and animal nutrition. Plants assimilate inorganic sulfate into two sulfur-containing amino acids, cysteine methionine. Low supply of leads to decreased sulfur pools within tissues. As sulfur-related metabolites represent integral part metabolism with multiple interactions, deficiency stress induces a number adaptive responses, which must be coordinated. To reveal the coordinating network adaptations deficiency, metabolite profiling...
Abstract Cinnamoyl-CoA reductase (CCR) catalyzes the penultimate step in monolignol biosynthesis. We show that downregulation of CCR transgenic poplar (Populus tremula × Populus alba) was associated with up to 50% reduced lignin content and an orange-brown, often patchy, coloration outer xylem. Thioacidolysis, nuclear magnetic resonance (NMR), immunocytochemistry epitopes, oligolignol profiling indicated relatively more syringyl than guaiacyl units. The cohesion walls affected, particularly...
Abstract Phosphorus (P) is an essential element for plant growth. Crop production of common bean (Phaseolus vulgaris), the most important legume human consumption, often limited by low P in soil. Functional genomics were used to investigate global gene expression and metabolic responses plants grown under P-deficient P-sufficient conditions. showed enhanced root shoot ratio accompanied reduced leaf area net photosynthesis rates. Transcript profiling was performed through hybridization nylon...
Gas chromatography coupled to mass spectrometry (GC-MS) is one of the most widespread routine technologies applied large scale screening and discovery novel metabolic biomarkers. However, currently majority spectral tags (MSTs) remains unidentified due lack authenticated pure reference substances required for compound identification by GC-MS. Here, we accessed information on compounds stored in Golm Metabolome Database (GMD) apply supervised machine learning approaches classification MSTs...
Abstract Developmental senescence is a coordinated physiological process in plants and critical for nutrient redistribution from senescing leaves to newly formed sink organs, including young developing seeds. Progress has been made concerning the genes involved regulatory networks controlling senescence. The resulting complex metabolome changes during have not investigated detail yet. Therefore, we conducted comprehensive profiling of metabolites, pigments, lipids, sugars, amino acids,...
Massive changes in gene expression occur when plants are subjected to phosphorus (P) limitation, but the breadth of metabolic these conditions and their regulation is barely investigated. Nearly 350 primary secondary metabolites were profiled shoots roots P-replete P-deprived Arabidopsis thaliana wild type mutants central P-signalling components PHR1 PHO2, microRNA399 overexpresser. In type, levels 87 metabolites, including phosphorylated not 3-phosphoglycerate, decreased, whereas...
Artemisinin-based therapies are the only effective treatment for malaria, most devastating disease in human history. To meet growing demand artemisinin and make it accessible to poorest, an inexpensive rapidly scalable production platform is urgently needed. Here we have developed a new synthetic biology approach, combinatorial supertransformation of transplastomic recipient lines (COSTREL), applied introduce complete pathway artemisinic acid, precursor artemisinin, into high-biomass crop...