A5022019242- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- GABA and Rice Research
- Horticultural and Viticultural Research
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- Plant Pathogens and Fungal Diseases
- Plant Micronutrient Interactions and Effects
- Analytical Chemistry and Chromatography
- Advanced Chemical Sensor Technologies
- Rice Cultivation and Yield Improvement
- Light effects on plants
- Genetic Mapping and Diversity in Plants and Animals
- Plant responses to water stress
- Plant biochemistry and biosynthesis
- Plant Pathogens and Resistance
- Aluminum toxicity and tolerance in plants and animals
- Plant Genetic and Mutation Studies
- Algal biology and biofuel production
- Mycorrhizal Fungi and Plant Interactions
Max Planck Institute of Molecular Plant Physiology
2016-2025
Max Planck Society
2008-2021
Max Planck Institute of Molecular Physiology
2017-2019
University of Córdoba
2016
University of British Columbia
2016
Leibniz Institute of Plant Biochemistry
2009-2011
Kansas State University
2011
Ludwig-Maximilians-Universität München
2011
Institut des Sciences des Plantes de Montpellier
2010
Centre National de la Recherche Scientifique
2010
Abstract Motivation: Typical GC-MS-based metabolite profiling experiments may comprise hundreds of chromatogram files, which each contain up to 1000 mass spectral tags (MSTs). MSTs are the characteristic patterns ∼25–250 fragment ions and respective isotopomers, generated after gas chromatography (GC) by electron impact ionization (EI) separated chemical molecules. These subsequently detected time-of-flight (TOF) spectrometry (MS). typically reported as a list ions, characterized mass,...
Abstract Cinnamoyl-CoA reductase (CCR) catalyzes the penultimate step in monolignol biosynthesis. We show that downregulation of CCR transgenic poplar (Populus tremula × Populus alba) was associated with up to 50% reduced lignin content and an orange-brown, often patchy, coloration outer xylem. Thioacidolysis, nuclear magnetic resonance (NMR), immunocytochemistry epitopes, oligolignol profiling indicated relatively more syringyl than guaiacyl units. The cohesion walls affected, particularly...
Abstract Phosphorus (P) is an essential element for plant growth. Crop production of common bean (Phaseolus vulgaris), the most important legume human consumption, often limited by low P in soil. Functional genomics were used to investigate global gene expression and metabolic responses plants grown under P-deficient P-sufficient conditions. showed enhanced root shoot ratio accompanied reduced leaf area net photosynthesis rates. Transcript profiling was performed through hybridization nylon...
Abstract Developmental senescence is a coordinated physiological process in plants and critical for nutrient redistribution from senescing leaves to newly formed sink organs, including young developing seeds. Progress has been made concerning the genes involved regulatory networks controlling senescence. The resulting complex metabolome changes during have not investigated detail yet. Therefore, we conducted comprehensive profiling of metabolites, pigments, lipids, sugars, amino acids,...
Massive changes in gene expression occur when plants are subjected to phosphorus (P) limitation, but the breadth of metabolic these conditions and their regulation is barely investigated. Nearly 350 primary secondary metabolites were profiled shoots roots P-replete P-deprived Arabidopsis thaliana wild type mutants central P-signalling components PHR1 PHO2, microRNA399 overexpresser. In type, levels 87 metabolites, including phosphorylated not 3-phosphoglycerate, decreased, whereas...
Artemisinin-based therapies are the only effective treatment for malaria, most devastating disease in human history. To meet growing demand artemisinin and make it accessible to poorest, an inexpensive rapidly scalable production platform is urgently needed. Here we have developed a new synthetic biology approach, combinatorial supertransformation of transplastomic recipient lines (COSTREL), applied introduce complete pathway artemisinic acid, precursor artemisinin, into high-biomass crop...
Heat and drought stress are projected to become major challenges sustain rice (Oryza sativa L.) yields with global climate change. Both stresses lead yield losses when they coincide flowering. A significant knowledge gap exists in the mechanistic understanding of responses floral organs that determine reproductive success under stress. Our work connects metabolomic transcriptomic changes anthers, pistils before pollination pollinated a heat-tolerant (N22) heat-sensitive (Moroberekan)...
Oxygen can fall to low concentrations within plant tissues, either because of environmental factors that decrease the external oxygen concentration or movement through tissues cannot keep pace with rate consumption. Recent studies document plants their consumption in response relatively small changes avoid internal anoxia. The molecular mechanisms underlying this have not been identified yet. aim study was use transcript and metabolite profiling investigate genomic arabidopsis roots a mild...
Summary The model legume Lotus japonicus was subjected to non‐lethal long‐term salinity and profiled at the ionomic, transcriptomic metabolomic levels. Two experimental designs with various stress doses were tested: a gradual step acclimatization an initial approach. Ionomic profiling by inductively coupled plasma/atomic emission spectrometry (ICP‐AES) revealed salt stress‐induced reductions in potassium, phosphorus, sulphur, zinc molybdenum. Microarray using Genechip ® allowed...
The plant parasitic beet cyst nematode Heterodera schachtii induces syncytial feeding structures in Arabidopsis roots. form strong sink tissues that have been suggested to be metabolically highly active. In the present study, metabolic profiling and gene targeted expression analyses were performed order study local systemic effects of infection on host. results showed increased levels many amino acids phosphorylated metabolites syncytia, as well high accumulation specific sugars such...
Abstract Phosphorus (P) deficiency is widespread in regions where the common bean (Phaseolus vulgaris), most important legume for human consumption, produced, and it perhaps factor that limits nitrogen fixation. Global gene expression metabolome approaches were used to investigate responses of nodules from plants inoculated with Rhizobium tropici CIAT899 grown under P-deficient P-sufficient conditions. showed drastic reduction nodulation nitrogenase activity as determined by acetylene assay....
Vitis vinifera berries are sensitive towards infection by the necrotrophic pathogen Botrytis cinerea, leading to important economic losses worldwide. The combined analysis of transcriptome and metabolome associated with fungal has not been performed previously in grapes or another fleshy fruit. In an attempt identify molecular metabolic mechanisms infection, peppercorn-sized fruits were infected field. Green veraison collected following for microarray complemented profiling primary other...
ABSTRACT Water limitation has become a major concern for agriculture. Such constraints reinforce the urgent need to understand mechanisms by which plants cope with water deprivation. We used non‐targeted metabolomic approach explore plastic systems responses non‐lethal drought in model and forage legume species of Lotus genus. In Lotus. japonicus , increased stress caused gradual increases most soluble small molecules profiled, reflecting global progressive reprogramming metabolic pathways....
A selection of 21 rice cultivars (Oryza sativa L. ssp. indica and japonica) was characterized under moderate long-term drought stress by comprehensive physiological analyses determination the contents polyamines selected metabolites directly related to polyamine metabolism. To investigate potential regulation biosynthesis at transcriptional level, expression genes encoding enzymes involved in these pathways were analyzed qRT-PCR. Analysis genomic loci revealed that 11 located drought-related...
Abstract Increasing grain sink strength by improving assimilate uptake capacity could be a promising approach toward getting higher yield. The barley (Hordeum vulgare) sucrose transporter HvSUT1 (SUT) was expressed under control of the endosperm-specific Hordein B1 promoter (HO). Compared with wild type, transgenic HOSUT grains take up more (Suc) in vitro, showing that transgene is functional. Grain Suc levels are not altered, indicating fluxes influenced rather than steady-state levels....
Heterosis, or hybrid vigor, is one of the most important tools in plant breeding and has previously been demonstrated for freezing tolerance. Freezing tolerance an trait because it can limit geographical distribution plants their agricultural yield. Plants from temperate climates increase during exposure to low, non-freezing temperatures a process termed 'cold acclimation'. Metabolite profiling indicated major reprogramming metabolism cold, but remained unclear previous studies which these...
ABSTRACT The legume genus Lotus includes glycophytic forage crops and other species adapted to extreme environments, such as saline soils. Understanding salt tolerance mechanisms will contribute the discovery of new traits which may enhance breeding efforts towards improved performance legumes in marginal agricultural environments. Here, we used a combination ionomic gas chromatography‐mass spectrometry (GC‐MS)‐based metabolite profilings complete shoots (pooling leaves, petioles stems)...
We investigated the systems response of metabolism and growth after an increase in irradiance nonsaturating range algal model Chlamydomonas reinhardtii. In a three-step process, photosynthesis levels metabolites increased immediately, 10 to 15 min, transcript protein abundance responded by 40 120 240 respectively. first phase, starch provided transient buffer for carbon until increased. This uncouples from fluctuating light environment. second phases, rising metabolite polysome loading drove...
One of the objectives plant translational genomics is to use knowledge and genes discovered in model species improve crops. However, value breeding, especially for complex traits like abiotic stress tolerance, remains uncertain. Using comparative (ionomics, transcriptomics metabolomics) we analyzed responses salinity three cultivated legume genus Lotus. At physiological ionomic levels, models responded a similar way crop species, changes concentration shoot Cl− correlated well with...
• Variations in tissue development and spatial composition have a major impact on the nutritional organoleptic qualities of ripe fleshy fruit, including melon (Cucumis melo). To gain deeper insight into mechanisms involved these changes, we identified key metabolites for rational food quality design. The metabolome, volatiles mineral elements were profiled employing an unprecedented range complementary analytical technologies. Fruits followed at number time points during final ripening...
Abstract Photosynthesis-related pathways are regarded as a promising avenue for crop improvement. Whilst empirical studies have shown that photosynthetic efficiency is higher in microalgae than C 3 or 4 crops, the underlying reasons remain unclear. Using tailor-made microfluidics labelling system to supply 13 CO 2 at steady state, we investigated vivo kinetics intermediates of Calvin Benson cycle and sugar, starch, organic acid amino synthesis pathways, protein lipids, Chlamydomonas...