A5028190672- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Phytochemicals and Antioxidant Activities
- Lignin and Wood Chemistry
- Biofuel production and bioconversion
- Metabolomics and Mass Spectrometry Studies
- Plant tissue culture and regeneration
- Genomics, phytochemicals, and oxidative stress
- Essential Oils and Antimicrobial Activity
- Seed Germination and Physiology
- Fermentation and Sensory Analysis
- Chromatography in Natural Products
- Photosynthetic Processes and Mechanisms
- Enzyme-mediated dye degradation
- Natural product bioactivities and synthesis
- Plant Stress Responses and Tolerance
- Natural Antidiabetic Agents Studies
- Botanical Research and Chemistry
- Phytochemistry and Biological Activities
- Vector-borne infectious diseases
- Ecology and Vegetation Dynamics Studies
- Nuts composition and effects
- Biochemical and biochemical processes
- Tree Root and Stability Studies
- Plant-derived Lignans Synthesis and Bioactivity
Biologie des Plantes et Innovation
2013-2023
Université de Picardie Jules Verne
2013-2023
UMR Transfrontalière BioEcoAgro
2020-2023
Institut Charles Viollette
2023
École Polytechnique
2023
Centre National de la Recherche Scientifique
2022
University of British Columbia
2007-2015
Vlaams Instituut voor Biotechnologie
2007-2010
Ghent University
2002-2010
Great Lakes Bioenergy Research Center
2010
Abstract Cinnamoyl-CoA reductase (CCR) catalyzes the penultimate step in monolignol biosynthesis. We show that downregulation of CCR transgenic poplar (Populus tremula × Populus alba) was associated with up to 50% reduced lignin content and an orange-brown, often patchy, coloration outer xylem. Thioacidolysis, nuclear magnetic resonance (NMR), immunocytochemistry epitopes, oligolignol profiling indicated relatively more syringyl than guaiacyl units. The cohesion walls affected, particularly...
Lignin is an important component of secondarily thickened cell walls. Cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) are two key enzymes that catalyse the penultimate last steps in biosynthesis monolignols. Downregulation CCR tobacco (Nicotiana tabacum) has been shown to reduce lignin content, whereas downregulated for CAD incorporates more aldehydes. We show altering expression either or both genes far-reaching consequences on transcriptome metabolome. cDNA-amplified...
Although the primary structure of proteins, nucleic acids, and carbohydrates can be readily determined, no sequencing method has been described yet for second most abundant biopolymer on earth (i.e. lignin). Within secondary-thickened plant cell walls, lignin forms an aromatic mesh arising from combinatorial radical-radical coupling monolignols many other less monomers. This polymerization process leads to a plethora units linkage types that affect physicochemical characteristics wall....
Drought stress is perhaps the most commonly encountered abiotic plants experience in natural environment, and it one of important factors limiting plant productivity. Here, we employed untargeted metabolite profiling to examine four drought-stressed hybrid poplar (Populus spp.) genotypes for their content, using gas chromatography coupled mass spectrometry. The primary objective these analyses was characterize profile trees assess relative drought resistance investigate underlying...
Drought has a major impact on tree growth and survival. Understanding responses to this stress can have important application in both conservation of forest health, production forestry. Trees the genus Populus provide an excellent opportunity explore mechanistic underpinnings drought responses, given growing molecular resources that are available for taxon. Here, foliar tissue six water-deficit stressed P. balsamifera genotypes was analysed variation metabolome response time day by using...
• Autumnal cold acclimation in conifers is a complex process, the timing and extent of which vary widely along latitudinal gradients for many tree species reflect local adaptation to climate. Although previous studies have detailed some aspects metabolic remodelling that accompanies conifers, little known about global dynamics, or how these changes among phenotypically divergent populations. Using untargeted GC-MS metabolite profiling, we monitored dynamics during autumnal three populations...
Abstract Histochemical screening of a flax ethyl methanesulfonate population led to the identification 93 independent M2 mutant families showing ectopic lignification in secondary cell wall stem bast fibers. We named this core collection Linum usitatissimum (flax) lbf mutants for lignified fibers and believe that represents novel biological resource investigating how fiber plants regulate lignin biosynthesis. As proof concept, we characterized lbf1 showed content increased by 350% outer...
Leaves of Pistacia lentiscus were collected from two Algerian sites in the mountains and littoral Tizi-Ouzou region. The harvest was conducted four consecutive seasons on same selected set trees. Essential oils (EOs) extracted by hydrodistillation; then, they analyzed gas chromatography coupled mass spectrometry (GC-MS). Forty-seven constituents could be detected quantified, including α-pinene (2-13%), β-caryophyllene (8-25%), β-myrcene (0.3-19%), bornyl acetate (0.8-7%), δ-cadinene (3-8%),...
Summary Somatic embryogenesis in gymnosperms is an effective approach to clonally propagating germplasm. However, embryogenic cultures frequently lose regenerative capacity. The interactions between metabolic composition, physiological state, genotype and capacity Pinus taeda (loblolly pine) somatic were explored using metabolomics. A stepwise modelling procedure, the Bayesian information criterion, generated a 47 metabolite predictive model that could explain culture productivity. performed...
Despite the scientific and economic importance of maize, little is known about its specialized metabolism. Here, five maize organs were profiled using different reversed-phase liquid chromatography-mass spectrometry methods. The resulting spectral metadata, combined with candidate substrate-product pair (CSPP) networks, allowed structural characterization 427 5,420 compounds, including phenylpropanoids, flavonoids, benzoxazinoids, auxin-related among others. Only 75 compounds already...
Abstract The objective of this work was the development a detailed, extensive and reliable database metabolomes P. vittata . Using an ultra‐performance liquid chromatography–triple quadrupole tandem mass spectrometry system (UPLC‐QqQ‐MS/MS) based on knowledge retention time spectral characteristics in‐house collection authentic standards, we screened for presence large natural compounds. represents 359 authenticated metabolites, comprising 220 primary 139 secondary metabolites (70...
The advances in 'high-throughput' biology have significantly expanded our fundamental understanding of complex biological processes inherent to tree growth and development. Relative the significant achievements attained with whole genome re-sequencing transcriptomics efforts, development power post-transcriptional tools such as proteomics metabolomics continue lag behind biology. However, inclusion these powerful functional genomics platforms should substantially enable systems assessments...
Cell wall localized heterogeneous polyesters are widespread in land plants. The composition of these polyesters, such as cutin, suberin, or more plant-specific forms the flax seed coat lignan macromolecule, can be determined after total hydrolysis ester linkages. main bottleneck structural characterization macromolecules, however, resides determination higher order monomer sequences. Partial hydrolysates release a complex mixture fragments different lengths, each present low abundance and...