A5030110427- Plant tissue culture and regeneration
- Food composition and properties
- Horticultural and Viticultural Research
- Plant-Microbe Interactions and Immunity
- Natural Fiber Reinforced Composites
- Botanical Studies and Applications
- Biofuel production and bioconversion
- Plant Gene Expression Analysis
- Seed Germination and Physiology
- Chromosomal and Genetic Variations
- Phytochemicals and Antioxidant Activities
- Textile materials and evaluations
- Plant biochemistry and biosynthesis
- Botanical Research and Chemistry
- Phytoestrogen effects and research
- Phytochemistry and Biological Activities
- Plant Genetic and Mutation Studies
- Lignin and Wood Chemistry
- Genomics, phytochemicals, and oxidative stress
- Plant Pathogenic Bacteria Studies
- Pharmacogenetics and Drug Metabolism
- Tree Root and Stability Studies
- Studies on Chitinases and Chitosanases
- Fungal Biology and Applications
- Pesticide and Herbicide Environmental Studies
Centre National de la Recherche Scientifique
2017-2024
Université de Lille
2017-2024
Unité de Glycobiologie Structurale et Fonctionnelle
2017-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2023
Stress Abiotiques et Différenciation des Végétaux Cultivés
2008-2014
Université Lille Nord de France
2009-2014
Vlaams Instituut voor Biotechnologie
2012
Ghent University
2012
UCLouvain
2000-2005
Unité de Recherche Pluridisciplinaire Prairies et Plantes Fourragères
1995
Abstract Nicotiana plumbaginifolia NpPDR1, a plasma membrane pleiotropic drug resistance-type ATP-binding cassette transporter formerly named NpABC1, has been suggested to transport the diterpene sclareol, an antifungal compound. However, direct evidence for role of resistance transporters in plant defense is still lacking. In situ immunolocalization and histochemical analysis using gusA reporter gene showed that NpPDR1 was constitutively expressed whole root, leaf glandular trichomes,...
Comparative genomics analysis unravels lineage-specific bursts of gene duplications related to the emergence specialized pathways. The CYP76C subfamily cytochrome P450 enzymes is specific Brassicaceae. Two its members were recently associated with monoterpenol metabolism. This prompted us investigate genetic and functional diversification. Our study revealed high rates duplication loss in Brassicaceae, suggesting association species-specific adaptive functions. Gene differential expression...
Abstract Flax (Linum usitatissimum) stems contain cells showing contrasting cell wall structure: lignified in inner stem xylem tissue and hypolignified outer bast fibers. We hypothesized that hypolignification should be associated with extensive phenolic accumulation used metabolomics transcriptomics to characterize these two tissues. 1H nuclear magnetic resonance clearly distinguished tissues identified different primary secondary metabolites, including coniferin p-coumaryl alcohol...
Flax dew-retting is a key step in the industrial extraction of fibers from flax stems and dependent upon production battery hydrolytic enzymes produced by micro-organisms during this process. To explore diversity dynamics bacterial fungal communities involved process we applied high-throughput sequencing (HTS) DNA metabarcoding approach (16S rRNA/ITS region, Illumina Miseq) on plant soil samples obtained over period 7 weeks July August 2014. Twenty-three six phyla were identified 11 four...
Abstract Histochemical screening of a flax ethyl methanesulfonate population led to the identification 93 independent M2 mutant families showing ectopic lignification in secondary cell wall stem bast fibers. We named this core collection Linum usitatissimum (flax) lbf mutants for lignified fibers and believe that represents novel biological resource investigating how fiber plants regulate lignin biosynthesis. As proof concept, we characterized lbf1 showed content increased by 350% outer...
Abstract Background Flax ( Linum usitatissimum L.) is an economically important fiber and oil crop that has been grown for thousands of years. The genome recently sequenced transcriptomics are providing information on candidate genes potentially related to agronomically-important traits. In order accelerate functional characterization these we have generated a flax EMS mutant population can be used as TILLinG (Targeting Induced Local Lesions in Genomes) platform forward reverse genetics....
Phenolic contents of the ethyl acetate extracts prepared from floral buds and opened flowers harvested on Crataegus azarolus trees native in two localities were performed. The antioxidant activity was measured by DPPH' (2,2-diphenyl-picrylhydrazyl), ABTS+ (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals scavenging using spectrophotometric method. C. var. aronia (Willd.) Batt., producing yellow fruits, richer total phenols (1638.7 +/- 89.9 mg acid gallic/100 g dry weight)...
Expression of NpABC1, a gene encoding plasma membrane ATP binding cassette (ABC) transporter in Nicotiana plumbaginifolia, is induced by sclareol, an antifungal diterpene produced at the leaf surface, as well sclareolide, close analog. A genomic fragment including 1282-bp region upstream NpABC1 transcription start was fused to reporter beta-glucuronidase (gus) and introduced into N. tabacum BY2 cells for stable transformation. 25-fold increase gus expression observed when were treated with...
A “novel” protocol is presented for easy and reliable estimation of soluble hydroxycinnamate levels in Cichorium intybus L. leaf tissue large-scale experiments. Samples were standardized by punching 6 discs per leaf, hydroxycinnamates extracted submerging the 80% ethanol with 5% acetic acid at least 48 h darkness 4°C. Residual dry mass was used a posteriori correction compound levels. Chlorophyll eliminated chloroform, aqueous phases transferred to microplates, dried, dissolved 50% methanol...
SummaryFruits from genotypes of 14 Crataegus were collected at the full-ripe stage three locations in Tunisia. Fresh fruit juice was analysed for some chemical properties. The content [% (v/w)], pH, titratable acidity (g malic acid 100 ml−1), and formol index (ml NaOH ml−1 juice), contents total soluble solids (°Brix), sugars [(g g−1 fresh weight (FW)], reducing FW), salts (mg established as: 2.0 – 11.9%, 3.2 4.2, 0.9 1.9, 2.8 4.4, 16.3 21.5, 5.3 17.0, 5.8 7.9, 0.6 0.7, respectively. also...
One of the biggest challenges for a more widespread utilization plant fibers is to better understand different molecular factors underlying variability in fineness and mechanical properties both elementary scutched fibers. Accordingly, we analyzed genome-wide transcription profiling from bast fiber bearing tissues seven flax varieties (4 spring, 2 winter 1 linseed) identified 1041 differentially expressed genes between varieties, which 97 were related cell wall metabolism. KEGG analysis...
niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français étrangers, laboratoires publics privés.
The mechanical properties of single flax fibers are characterized here as a function dew retting. measured using micromechanical and nanomechanical techniques over large retting period (91 days). Damage-free in various stages were manually extracted from retted plant stems. flexural modulus strength the determined methods. effective outer surface was AFM-based nanoindentation. methods revealed that does not vary significantly progresses. two distinct values attributed to different failure...