A5056386459- Lipid Membrane Structure and Behavior
- Drug Transport and Resistance Mechanisms
- Cholesterol and Lipid Metabolism
- Advanced Fluorescence Microscopy Techniques
- RNA Interference and Gene Delivery
- Caveolin-1 and cellular processes
- Erythrocyte Function and Pathophysiology
- Transgenic Plants and Applications
- Spectroscopy and Quantum Chemical Studies
- Plant tissue culture and regeneration
- Piperaceae Chemical and Biological Studies
- Toxin Mechanisms and Immunotoxins
- Advanced biosensing and bioanalysis techniques
- Trace Elements in Health
- Blood properties and coagulation
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Lipid metabolism and disorders
- Plant-Microbe Interactions and Immunity
- Plant Genetic and Mutation Studies
- RNA Research and Splicing
- Plant Pathogenic Bacteria Studies
- Histone Deacetylase Inhibitors Research
Medical University of Lublin
2022-2025
University of Wrocław
2019-2023
Łukasiewicz Research Network – PORT Polish Center for Technology Development
2022-2023
Wrocław University of Science and Technology
2020
AGH University of Krakow
2020
UCLouvain
2005-2013
Centre National de la Recherche Scientifique
2011-2012
Inserm
2009-2012
Centre d’Immunologie de Marseille-Luminy
2009-2012
Aix-Marseille Université
2012
Abstract Nicotiana plumbaginifolia NpPDR1, a plasma membrane pleiotropic drug resistance-type ATP-binding cassette transporter formerly named NpABC1, has been suggested to transport the diterpene sclareol, an antifungal compound. However, direct evidence for role of resistance transporters in plant defense is still lacking. In situ immunolocalization and histochemical analysis using gusA reporter gene showed that NpPDR1 was constitutively expressed whole root, leaf glandular trichomes,...
Signaling through inhibitory NK cell receptors determines the distribution, and hence responsiveness, of activating receptors.
Pleiotropic drug resistance (PDR) transporters are a group of membrane proteins belonging to the ABCG sub-family ATP binding cassette (ABC) transporters. There is clear evidence for involvement plant ABC in fungal and bacterial pathogens, but not biotic stress response insect or herbivore attack. Here, we describe PDR transporter, ABCG5/PDR5, from Nicotiana tabacum. GFP fusion subcellular fractionation studies revealed that ABCG5/PDR5 localized plasma membrane. Staining transgenic plants...
The ABCA1 transporter orchestrates cellular lipid homeostasis by promoting the release of cholesterol to plasmatic acceptors. molecular mechanism is, however, unknown. We report here on biophysical analysis in living HeLa cells microenvironment at plasma membrane. modifications membrane attributes induced were assessed both outer and inner leaflet monitoring either lifetime inserted fluorescent analogues fluorescence imaging microscopy (FLIM) or, respectively, translocation cationic sensors....
SUMMARY The behaviour of Nicotiana plumbaginifolia plants silenced for the ATP-binding cassette transporter gene NpPDR1 was investigated in response to fungal and oomycete infections. importance plant defence demonstrated two organs which is constitutively expressed: roots petal epidermis. plantlets lines expression were clearly more sensitive than those controls pathogens Botrytis cinerea, Fusarium oxysporum sp., F. f. sp. nicotianae, melonis Rhizoctonia solani, as well pathogen...
MPP1 (membrane palmitoylated protein 1) belongs to the MAGUK (membrane-associated guanylate kinase homologs) scaffolding family. These proteins organize molecules into complexes, thereby maintaining structural heterogeneity of plasma membrane (PM). Our previous results indicated that direct, high-affinity interactions between and flotillins (raft marker proteins) display dominant PM-modulating capacity in erythroid cells. In this study, with high-resolution structured illuminated imaging, we...
Amphotericin B (AmB) belongs to a group of polyene antibiotics commonly used in the treatment systemic mycotic infections. A widely accepted mechanism action AmB is based on formation an oligomeric pore structure within plasma membrane (PM) by interaction with sterols. Although binds preferentially ergosterol, it can also bind cholesterol mammalian PM and cause severe cellular toxicity. The lipid content its lateral organization at cell appear be significant for binding. Several ATP-binding...
Dynamic biological processes in living cells, including those associated with plasma membrane organization, occur on various spatial and temporal scales, ranging from nanometers to micrometers microseconds minutes, respectively. Such a broad range of challenges conventional microscopy approaches. Here, we detail the procedure for implementing spot variation Fluorescence Correlation Spectroscopy (svFCS) measurements using classical fluorescence microscope that has been customized. The...
Abstract Background Melanoma is one of the most aggressive and deadliest skin tumor. Cholesterol content in melanoma cells elevated, a portion it accumulates into lipid rafts. Therefore, plasma membrane cholesterol its lateral organization might be directly linked with tumor development. ATP Binding Cassette A1 (ABCA1) transporter modulates physico-chemical properties by modifying distribution. Several studies activity different outcome progression depending on which type. However, no direct...
Membrane rafts play a crucial role in the regulation of many important biological processes. Our previous data suggest that specific interactions flotillins with MPP1 are responsible for membrane raft domain organization and erythroid cells. Interaction flotillin-based protein network components underlies mechanism formation regulation, including cells low expression MPP1.We sought to identify other flotillin partners via immobilized recombinant flotillin-2-based affinity approach mass...
Extensive studies showed the crucial role of ATP binding cassette (ABC) transporter ABCA1 in organizing lipid microenvironment at plasma membrane (PM) living cells. However, exact this protein terms redistribution and lateral reorganization PM is still being discussed. Here, we took advantage spot variation fluorescence correlation spectroscopy (svFCS) to investigate molecular dynamics expressed Chinese hamster ovary cells (CHO-K1). We confirmed that strongly confined into raft nanodomains....
Our goal is to obtain a comprehensive description of molecular processes occurring at cellular membranes in different biological functions. We aim characterizing the complex organization and dynamics plasma membrane single-molecule level, by developing analytic tools dedicated Single-Particle Tracking (SPT) high density: Multiple-Target Tracing (MTT)1. Single-molecule videomicroscopy, offering millisecond nanometric resolution1-11, allows detailed representation organization12-14 accurately...
Colloidal semiconductor quantum dots (QD), as well other nanoparticles, are useful in cell studies fluorescent labels. They may also be used more active components various cellular assays, serving sensors or effectors. However, not all QDs biocompatible. One of the main problems is their outer coat, which needs to stable and sustain hydrophilicity. Here we show that purpose-designed CdSe QDs, covered with a Puf protein, can efficiently accumulated by HeLa cells. The uptake was measurable...