A5025228961- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Toxin Mechanisms and Immunotoxins
- Piperaceae Chemical and Biological Studies
- Transgenic Plants and Applications
- Yersinia bacterium, plague, ectoparasites research
- Plant pathogens and resistance mechanisms
- Plant tissue culture and regeneration
- Banana Cultivation and Research
- Insect and Pesticide Research
- Photosynthetic Processes and Mechanisms
- Microbial Metabolism and Applications
- Burkholderia infections and melioidosis
- Drug Transport and Resistance Mechanisms
- Plant Genetic and Mutation Studies
- Fungal Plant Pathogen Control
- Genomics and Phylogenetic Studies
- Ginger and Zingiberaceae research
- Plant Pathogens and Resistance
- Paraquat toxicity studies and treatments
Walloon Agricultural Research Centre
2007-2023
UCLouvain
2005
Gembloux Agro-Bio Tech
1999-2001
Abstract Nicotiana plumbaginifolia NpPDR1, a plasma membrane pleiotropic drug resistance-type ATP-binding cassette transporter formerly named NpABC1, has been suggested to transport the diterpene sclareol, an antifungal compound. However, direct evidence for role of resistance transporters in plant defense is still lacking. In situ immunolocalization and histochemical analysis using gusA reporter gene showed that NpPDR1 was constitutively expressed whole root, leaf glandular trichomes,...
Pleiotropic drug resistance (PDR) transporters are a group of membrane proteins belonging to the ABCG sub-family ATP binding cassette (ABC) transporters. There is clear evidence for involvement plant ABC in fungal and bacterial pathogens, but not biotic stress response insect or herbivore attack. Here, we describe PDR transporter, ABCG5/PDR5, from Nicotiana tabacum. GFP fusion subcellular fractionation studies revealed that ABCG5/PDR5 localized plasma membrane. Staining transgenic plants...
ABSTRACT Toxin-based identification procedures are useful for differentiating Pseudomonas syringae pathovars. A biological test on peptone-glucose-NaCl agar in which the yeast Rhodotorula pilimanae was used proved to be more reliable detecting lipodepsipeptide-producing strains of P. than usual potato dextrose Geotrichum candidum is used. PCR performed with primers designed amplify a 1,040-bp fragment coding sequence syrD gene, assumed involved syringomycin and syringopeptin secretion,...
ABSTRACT The siderophore and virulence factor yersiniabactin is produced by Pseudomonas syringae . Yersiniabactin was originally detected high-pressure liquid chromatography (HPLC); commonly used PCR tests proved ineffective. production in P. correlated with the possession of irp1 located a predicted locus. Three similarly divergent locus groups were determined: Yersinia pestis group, Photorhabdus luminescens group; organization similar In pv. tomato DC3000, has high GC content (63.4%...
Comparative genomics of several strains Erwinia amylovora, a plant pathogenic bacterium causal agent fire blight disease, revealed that its diversity is primarily attributable to the flexible genome comprised plasmids. We recently identified and sequenced in full novel 65.8 kb plasmid, called pEI70. Annotation lack known virulence-related genes, but found evidence for unique integrative conjugative element related other human pathogens. analyses using BLASTN showed pEI70 almost entirely...
ABSTRACT Nonfluorescent highly virulent strains of Pseudomonas syringae pv. aptata isolated in different European countries and Uruguay produce a nonfluorescent peptide siderophore, the production which is iron repressed specific to these strains. The amino acid composition this siderophore identical that dominant fluorescent produced by P. strains, molecular masses respective Fe(III) chelates are 1,177 1,175 atomic mass units. unchelated converted into at pH 10, colors spectral...
The production of peptide siderophores and the variation in siderophore among strains Pseudomonas syringae viridiflava were investigated. An antibiose test was used to select a free amino acid-containing agar medium favorable for fluorescent by two P. strains. A culture technique which both liquid solid asparagine-containing media proved be reproducible highly effective inducing Using asparagine as carbon source appeared favor production, relatively high levels produced when certain acids...
SUMMARY The behaviour of Nicotiana plumbaginifolia plants silenced for the ATP-binding cassette transporter gene NpPDR1 was investigated in response to fungal and oomycete infections. importance plant defence demonstrated two organs which is constitutively expressed: roots petal epidermis. plantlets lines expression were clearly more sensitive than those controls pathogens Botrytis cinerea, Fusarium oxysporum sp., F. f. sp. nicotianae, melonis Rhizoctonia solani, as well pathogen...
ABSTRACT The relationship of pyoverdins produced by 41 pathovars Pseudomonas syringae and phytopathogenic species was investigated. A high-performance liquid chromatography method for analyzing the culture medium proved to be superior isoelectric focusing detecting pyoverdin production, differentiating slightly different pyoverdins, atypical from typical Fe(III)-chelated pyoverdins. Nonfluorescent strains were found in amygdali , meliae fuscovaginae P. syringae. agarici marginalis Among...
The structure elucidation of the cyclic (lactonic) forms pyoverdins with a succinamide side chain originally produced by closely related species Pseudomonas syringae and P. cichorii is reported. Mass spectrometry nuclear magnetic resonance analyses as well determination configuration amino acids after degradation indicate that these two differ only replacement first in-chain serine glycine. dihydropyoverdin can be used both siderophores.
Fosetyl‐Al (the aluminium salt of ethyl‐phosphite) is an acid product used to fight oomycete diseases and sometimes against bacterial diseases, but its antibacterial mode action largely unknown. Therefore, the direct effects fosetyl‐Al neutralized on colonization leaf surfaces by Pseudomonas syringae were compared. Control was highest almost complete in acidic conditions (99.7%), showing importance acidity for maximal efficacy plant surfaces. However, high inhibition obtained with (88.9%)...
In late August 2019, symptoms consistent with those of common bacterial blight were observed on mature beans (Phaseolus vulgaris) several varieties in a trial plot Hesbaye the centre Belgium. On leaves, irregular brown spots surrounded by yellow halo and progressed to give burnt appearance (Fig. 1); pods, water-soaked developed into reddish-brown 2). Symptoms scattered throughout (approx. 100 m2), harvesting was abandoned due pod infection. Isolations made from margins lesions. Tissues...
Outbreaks in Europe have raised concerns about poinsettia bacterial canker caused by Curtobacterium flaccumfaciens pv. poinsettiae, described the United States. Using a semiselective medium containing aztreonam and fosfomycin selection during isolation based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, 424 strains were isolated from Belgian poinsettias of African European origin. Different populations coexisted: 130 identified as C....