A5034420465- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Fungal Diseases
- Phytoplasmas and Hemiptera pathogens
- Plant-Microbe Interactions and Immunity
- Banana Cultivation and Research
- Plant Virus Research Studies
- Entomopathogenic Microorganisms in Pest Control
- Cocoa and Sweet Potato Agronomy
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Plant pathogens and resistance mechanisms
- Insect-Plant Interactions and Control
- Bacteriophages and microbial interactions
- Research on scale insects
- Insect and Pesticide Research
- Plant Physiology and Cultivation Studies
- Forest Insect Ecology and Management
- Horticultural and Viticultural Research
- Infections and bacterial resistance
- Plant Disease Resistance and Genetics
- Fungal Biology and Applications
- Genetically Modified Organisms Research
- Viral gastroenteritis research and epidemiology
- Scientific Measurement and Uncertainty Evaluation
- Electrowetting and Microfluidic Technologies
National Institute of Biology
2013-2024
Centre of Excellence for Biosensors, Instrumentation and Process Control
2015-2017
Agroscope
2013
Detection and quantification of plant pathogens in the presence inhibitory substances can be a challenge especially with environmental samples. Real-time quantitative PCR has enabled high-throughput detection pathogens; however, its use is linked to standardized reference materials, sensitivity inhibitors lead lower accuracy. Droplet digital been proposed as method overcome these drawbacks. Its absolute does not rely on standards tolerance demonstrated mostly clinical Such features would...
Real-time PCR is the technique of choice for nucleic acid quantification. In field detection genetically modified organisms (GMOs) quantification biotech products may be required to fulfil legislative requirements. However, successful depends crucially on quality sample DNA analyzed. Methods GMO are generally validated certified reference materials that in form powdered grain material, while routine laboratories must performed a wide variety matrixes. Due food processing, matrixes can...
In May 2013, 20 plants in a production orchard of kiwifruit (Actinidia deliciosa) cv. Hayward the seaside area Primorska showed small, angular, coalescing necrotic leaf spots and cankers on green shoots. following 2 weeks, disease progressed to wilting shoot dieback with exudates. Symptoms were consistent Pseudomonas syringae pv. actinidiae. Circular, flat, granulated colonies entire margins isolated from King's medium B (KB) sucrose nutrient agar boric acid, cephalexine, cycloheximide....
Genetic testing of tumor tissue and circulating cell-free DNA for somatic variants guides patient treatment many cancers. Such measurements will be fundamental in the future support precision medicine. However, there are currently no primary reference measurement procedures available nucleic acid quantification that would translation tests into routine use.We assessed accuracy digital PCR (dPCR) copy number a frequently occurring single-nucleotide variant colorectal cancer (KRAS c.35G>A,...
Specific and sensitive TaqMan real‐time PCR assays were developed targeting chromosomal DNA of Erwinia amylovora ( ams C gene ITS region). These increased the reliability detection E. strains, regardless their plasmid profile, have ability to differentiate between spp. strains from Hokkaido, pyrifoliae isolated necrotic pear blossoms in Spain. The used for testing efficiency three different extraction methods remove plant‐based inhibitors. Combined with an automated DNA‐extraction method...
Erwinia amylovora causes a major disease of pome fruit trees worldwide, and is regulated as quarantine organism in many countries. While some diversity isolates has been observed, molecular epidemiology this bacterium hindered by lack simple typing techniques with sufficiently high resolution. We report system E. based on variable number tandem repeats (VNTR) analysis. Repeats the genome were identified comparative genomic tools, VNTR markers developed validated. A Multiple-Locus Analysis...
The increased globalization of crops production and processing industries also promotes the side-effects more rapid efficient spread plant pathogens. To prevent associated economic losses, particularly those related to bacterial diseases where their management relies on removal infected material from production, simple, easy-to-perform, cost-effective tests are needed. Loop-mediated isothermal amplification (LAMP) assays that target 16S rRNA, fliC egl genes were compared evaluated as on-site...
Comparative genomics of several strains Erwinia amylovora, a plant pathogenic bacterium causal agent fire blight disease, revealed that its diversity is primarily attributable to the flexible genome comprised plasmids. We recently identified and sequenced in full novel 65.8 kb plasmid, called pEI70. Annotation lack known virulence-related genes, but found evidence for unique integrative conjugative element related other human pathogens. analyses using BLASTN showed pEI70 almost entirely...
Soft rot pathogenic bacteria from the genus Dickeya cause severe economic losses in orchid nurseries worldwide, and there is no effective control currently available. In last decade, has undergone multiple changes as new taxa have been described, just recently a putative species was reported. This study reports isolation of three bacteriophages active against novel spp. isolates commercially produced infected orchids that show variable host-range profiles. Bacteriophages were isolated...
Members of the genus Dickeya are plant pathogens that mostly cause soft‐rot diseases. Diversity within has led to recent description three novel species (i.e. a mainly clonal D . solani economic consequence in potatoes, D. aquatica from water samples and fangzhongdai isolated necrotic pear trees China). However, multilocus sequence analysis ( MLSA ) highlighted occurrence seven isolates not related defined species. These include previously unclassified group strains monocotyledonous plants,...
VALITEST is an EU‐funded project built to improve the reliability of diagnostic tests performed in plant health laboratories across European and Mediterranean region. The undertaken by a consortium 16 partners composed research institutions, private companies (such as kit providers), national protection organizations one intergovernmental organization (EPPO). Current harmonized procedures for validation test performance studies will be improved based on experience gained from including...
The Dickeya genus is part of the Pectobacteriaceae family that included in newly described enterobacterales order. It comprises a group aggressive soft rot pathogens with wide geographic distribution and host range. Among them, new fangzhongdai species groups causative agents maceration-associated diseases impact variety crops ornamentals. affects mainly monocot plants, but D. strains have also been isolated from pear trees water sources. Here, we analysed which genetic novelty exists this...
Bacterial soft rots are a serious limitation to the production of orchids and other horticultural plants. Here, characterization causative bacteria isolated from Phalaenopsis showing symptoms, commercial site, is reported. The most commonly were identified as Dickeya spp. Partial sequencing 16S rDNA , fliC dnaX showed diversity among isolates divided into two groups, with greatest similarity previously reported undefined lineages ( UDL ‐3 ‐4). Two (B16, S1) sequenced using next‐generation...
Apple proliferation (AP) phytoplasma ('Candidatus Phytoplasma mali'; Seemüller & Schneider, 2004) is the causal agent of a serious disease apple (Malus domestica). It has also been found in hazelnut (Corylus spp.) (Marcone et al., 1996), pear (Pyrus communis) and Japanese plum (Prunus salicina). In this report, AP was cherry avium), apricot (P. armeniaca) cherry, wilting, dying, floral phloem necrosis were first observed 2004 south western Slovenia. No phytoplasmas could be isolated from...
Ralstonia solanaceraum is the quarantine plant pathogenic bacterium that causes bacterial wilt in over 200 host plants, which include economically important crops such as potato, tomato, tobacco, banana, and ginger. Alternative biological methods of disease control can be used integrated pest management are extensively studied. In search new proteins with antibacterial activity against R. solanacearum, we identified L-amino acid oxidases (LAOs) from fruiting bodies Amanita phalloides (ApLAO)...
A real‐time PCR MGB‐probe‐based detection method specific to Xylophilus ampelinus , the cause of grapevine bacterial blight, was developed. Used in combination with DNeasy plant mini kit, sensitivity X. approximately 100 cells from tissue extracts, surpassing an existing nested at least tenfold. In field samples a high correlation observed between cycle threshold (Ct) values obtained and isolation on artificial media. Isolation successful Ct below 25. Lower concentrations up 36, could also...
The first droplet-digital-PCR-based absolute quantification of “flavescence dorée” phytoplasma, agent a quarantine phytoplasma yellows in grapevine was performed. Quantitative PCR that targets the secY gene transferred to droplet digital and used for without need calibration curves. sensitivity two assays compares well shown it could be quality control DNA based on in-house reference materials typically diagnostics metrological laboratories. This new tool has great potential monitoring...
In total, 150 protein extracts from 94 different basidiomycete and ascomycete wild mushroom species were tested for antibacterial activity against the quarantine plant-pathogen bacterium Ralstonia solanacearum. in vitro microtiter plate assays, 15 with moderate to high activities identified: 11 completely inhibited bacterial growth 4 showed partial inhibition. Of these extracts, 5 further 3 slowed disease progression reduced severity artificially inoculated tomato potato plants. However, of...