A5033443120- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Microfluidic and Bio-sensing Technologies
- CRISPR and Genetic Engineering
- Cell Image Analysis Techniques
- Metabolomics and Mass Spectrometry Studies
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Cancer Genomics and Diagnostics
- Congenital heart defects research
- Cardiac Fibrosis and Remodeling
- thermodynamics and calorimetric analyses
- Animal testing and alternatives
- Image Processing Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Genomics, phytochemicals, and oxidative stress
- Nitrogen and Sulfur Effects on Brassica
- Luminescence and Fluorescent Materials
Istituto Nazionale di Ricerca Metrologica
2013-2024
University of Manitoba
2012
The resazurin assay, also known as the Alamar Blue stands a cornerstone technique in cell biology, microbiology, and drug development. It assesses viability of cells through conversion into highly fluorescent resorufin. resulting fluorescence intensity provides reliable estimate viable numbers. Cytotoxicity assays, such resazurin-based method, play crucial role screening potential candidates assessment pharmaceutical chemical toxicity. In recent years, inconsistencies have arisen...
Genetic testing of tumor tissue and circulating cell-free DNA for somatic variants guides patient treatment many cancers. Such measurements will be fundamental in the future support precision medicine. However, there are currently no primary reference measurement procedures available nucleic acid quantification that would translation tests into routine use.We assessed accuracy digital PCR (dPCR) copy number a frequently occurring single-nucleotide variant colorectal cancer (KRAS c.35G>A,...
Modulation of microRNA expression holds the promise to achieve direct reprogramming fibroblasts into cardiomyocyte-like cells as a new strategy for myocardial regeneration after ischemic heart disease. Previous reports have shown that murine can be directly reprogrammed induced cardiomyocytes (iCMs) by transient transfection with four mimics (miR-1, 133, 208 and 499, termed "miRcombo"). Hence, study on effect miRcombo adult human cardiac (AHCFs) deserves attention in perspective future...
Main text Several scientific fields, such as cytology, biochemistry, molecular biology and genetics have cells their direct topic of study. The cell is a fundamental unit highly technological systems, employed, for example, in the development new chemicals drugs, evaluation compound toxicity, novel approaches to health, regenerative medicine. High content screening technologies are cellular imaging-based assays. In this framework, density, confluency size measurands that can describe...
Main text Nucleic acid amplification tests for SARS-CoV-2, the virus responsible COVID-19 pandemic, primarily target RNA as analyte. These detect presence of SARS-CoV-2 specific sequences, confirming infection through in vitro diagnostic methods. However, lack a standardized reference measurement system has led to varied units and unclear traceability reporting content quantities, complicating comparisons between different [1-5]. To address this challenge, pilot study CCQM-P199b was...
Abstract Cardiac fibrosis is one of the main causes heart failure, significantly contributing to mortality. The discovery and development effective therapies able heal fibrotic pathological symptoms thus remain paramount importance. Micro‐physiological systems (MPS) are recently introduced as promising platforms accelerate this finding. Here a 3D in vitro model human cardiac fibrosis, named uScar, developed by imposing cyclic mechanical stimulation atrial fibroblasts (AHCFs) cultured beating...
Coherent anti-Stokes Raman scattering (CARS) microscopy is combined with second harmonic generation (SHG) technique in order to follow the early stage of stem cell differentiation within a 3D scaffold. CARS can detect lipid membranes and droplet compartments living cells SHG enables strong imaging contrast for molecules non-centrosymmetric ordered structure like collagen. One first evidence hMSCs formation an extracellular matrix (ECM) where collagen protein its main component. This work...
In cell-based therapies, in vitro studies on biomimetic cell-scaffold constructs can facilitate the determination of cell dose, a key factor guaranteeing effectiveness treatment. However, highly porous scaffolds do not allow nondestructive evaluation number. Our objective was to develop method for human mesenchymal stem cells dose scaffold bone regeneration.Proliferation trend Biocoral® measured by resazurin-based assay here optimized 3D cultures. The allows noninvasively follow...
The restoration of cardiac functionality after myocardial infarction represents a major clinical challenge. Recently, we found that transient transfection with microRNA combination (miRcombo: miR-1, miR-133, miR-208 and 499) is able to trigger direct reprogramming adult human fibroblasts (AHCFs) into induced cardiomyocytes (iCMs) in vitro. However, achieving efficient still remains aim this study was investigate the influence tissue-like biochemical biophysical stimuli on efficiency....
Design of nanocarriers for efficient miRNA delivery can significantly improve miRNA-based therapies. Lipoplexes based on helper lipid, dioleoyl phosphatidylethanolamine (DOPE) and cationic lipid [2-(2,3-didodecyloxypropyl)-hydroxyethyl] ammonium bromide (DE) were formulated to efficiently deliver miR-1 or a combination four microRNAs (miRcombo) adult human cardiac fibroblasts (AHCFs). with amino-to-phosphate groups ratio 3 (N/P 3) showed nanometric hydrodynamic size (372 nm), positive...
In vitro models of pathological cardiac tissue have attracted interest as predictive platforms for preclinical validation therapies. However, reproducing specific features, such fibrosis size (i.e., thickness and width) stage development are missing. This research was aimed at engineering 2D 3D early-stage post-infarct fibrotic characterized by non-aligned organization) on bioartificial scaffolds with biomimetic composition, design, surface stiffness. random nanofibrous structure 150 µm...
Background Over 2,000 people a year in the United Kingdom need bone marrow or blood stem cell transplant. It is important to accurately quantify hematopoietic cells predict whether transplant will be successful replenishing immune system. However, they are present at low frequency, which complicates accurate quantification. The current gold standard method single‐platform flow cytometry using internal reference counting beads determine concentration of CD34 cells. volumetric cytometers have...
ABSTRACT Nucleic acid amplification tests including reverse transcription quantitative PCR (RT-qPCR) are used to detect RNA from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the causative agent of disease 2019 (COVID-19) pandemic. Standardized measurements can facilitate comparable performance laboratory in absence existing reference measurement systems early on a Interlaboratory study CCQM P199b “SARS-CoV-2 copy number quantification” was designed test fitness-for-purpose...
The A549 cell line has become a cornerstone in biomedical research, particularly cancer studies and serves as critical tool cytotoxicity drug screening where it is used to evaluate the impact of pharmaceutical compounds on cellular viability. One most widely adopted methods for viability assessment, which also evaluating cytotoxicity, resazurin-based assay. This assay exploits ability living cells convert resazurin into fluorescent resorufin, providing reliable indicator metabolic activity....
Magnetic oxygen-loaded nanodroplets (MOLNDs) are a promising class of nanomaterials sensitive to both ultrasound and magnetic field, which can be employed as carriers for drug delivery applications, particularly treating hypoxic tissue diseases.
ABSTRACT For the impact of genomic testing from liquid biopsies to be maximized, mechanisms ensure reproducible and comparable test performance will required. This can established maintained through reference measurement procedures materials with property values that are internationally traceability a common standard. To achieve this objective, an interlaboratory study was organised explore digital PCR (dPCR) for standardisation cell-free DNA (cfDNA) quantification. Blinded samples...
Cell counting is a fundamental procedure in living cell culture-based experiments and protocols which the number quantification required. The of cells one parameters necessary to investigate several culture features requiring be monitored as function time, such viability, proliferation, growth, fitness metabolism. Aim this paper contributing declare comprehensive uncertainty budget for through metabolic assays according EURACHEM/CITAC Guide Quantifying Uncertainty Analytical Measurement.
Abstract Direct reprogramming of fibroblasts into induced cardiomyocytes (iCMs) through microRNAs (miRNAs) is a new emerging strategy for myocardial regeneration after ischemic heart disease. Previous studies have reported that murine can be directly reprogrammed iCMs by transient transfection with four miRNAs (miRs-1, 133, 208 and 499 – termed “miRcombo”). While advancement in the knowledge direct cell molecular mechanism progress, it important to investigate if this may translated humans....
Main text Breast cancer is the most common malignant tumor in women all over world. cancers with amplification of human epidermal growth factor receptor 2 (HER2) gene (referred to as HER2-positive breast cancer) accounts for about 20% ~ 30% invasive cancer, and this type characterized by high invasiveness, risk recurrence, rapid progression poor prognosis. The determination HER2 expression or copy number variant (CNV) (HER2 normalized cell a reference gene) cells patients beneficial choice...