A5018068390- Virus-based gene therapy research
- CAR-T cell therapy research
- SARS-CoV-2 detection and testing
- Parvovirus B19 Infection Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- SARS-CoV-2 and COVID-19 Research
- Genomics and Chromatin Dynamics
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- Animal Virus Infections Studies
- Galectins and Cancer Biology
- Helicobacter pylori-related gastroenterology studies
- Viral Infectious Diseases and Gene Expression in Insects
- Ubiquitin and proteasome pathways
- Fungal and yeast genetics research
- Viral gastroenteritis research and epidemiology
- Cancer Research and Treatments
- RNA Research and Splicing
- Gender Studies in Language
- Advanced biosensing and bioanalysis techniques
- Animal Genetics and Reproduction
- Immunotherapy and Immune Responses
- Veterinary medicine and infectious diseases
- Cervical Cancer and HPV Research
- Microbial Metabolites in Food Biotechnology
German Cancer Research Center
2015-2024
Heidelberg University
2013-2024
Luxembourg Institute of Health
2016-2024
European Commission
2021-2024
University Hospital Heidelberg
2014
DKFZ-ZMBH Alliance
1998-2010
Universidade Federal de São Paulo
2010
Balboa Nephrology Medical Group
2003
Despite decades of research and the best up-to-date treatments, grade 4 Glioblastoma (GBM) remains uniformly fatal with a patient median overall survival less than 2 years. Recent advances in immunotherapy have reignited interest utilizing immunological approaches to fight cancer. However, current immunotherapies so far not met anticipated expectations, achieving modest results their journey from bench bedside for treatment GBM. Understanding intrinsic features GBM is crucial importance...
Mesenchymal stem cells (MSCs) are multipotent capable of differentiation into several mesodermal lineages. These have been isolated from various tissues, such as adult bone marrow, placenta, and fetal tissues. The comparative potential these originating different tissues to differentiate the chondrogenic lineage is still not fully defined. aim our study was investigate MSCs sources. were phenotypically characterized examined for their capacity, based on morphological criteria expression...
ABSTRACT The rat parvovirus H-1 (H-1PV) attracts high attention as an anticancer agent, because it is not pathogenic for humans and has oncotropic oncosuppressive properties. viral nonstructural NS1 protein thought to mediate H-1PV cytotoxicity, but its exact contribution this process remains undefined. In study, we analyzed the effects of on human cell proliferation viability. We show that expression sufficient induce accumulation cells in G 2 phase, apoptosis via caspase 9 3 activation,...
Multiple lineages of SARS-CoV-2 have been identified featuring distinct sets genetic changes that confer to the virus higher transmissibility and ability evade existing immunity. The continuous evolution may pose challenges for current treatment options diagnostic tools. In this study, we first evaluated performance 14 WHO-recommended real-time reverse transcription (RT)-PCR assays currently in use detection found only one assay has reduced against Omicron. We then developed a new duplex...
Mutations in the homeobox gene SHOX cause growth retardation and skeletal abnormalities associated with Léri-Weill, Langer, Turner syndromes. Little is known about mechanism underlying these SHOX-related inherited disorders of bone formation. Here we demonstrate that expression osteogenic stable cell lines, primary oral fibroblasts, chondrocytes leads to cycle arrest apoptosis. These events are alterations several cellular genes, including pRB, p53, cyclin kinase inhibitors p21Cip1 p27Kip1....
Main text Nucleic acid amplification tests for SARS-CoV-2, the virus responsible COVID-19 pandemic, primarily target RNA as analyte. These detect presence of SARS-CoV-2 specific sequences, confirming infection through in vitro diagnostic methods. However, lack a standardized reference measurement system has led to varied units and unclear traceability reporting content quantities, complicating comparisons between different [1-5]. To address this challenge, pilot study CCQM-P199b was...
We have attempted to express the Helicobacter pylori vacuolating cytotoxin in Escherichia coli. Although 95-kDa VacA polypeptide was expressed abundantly, it completely lacked any biological activity. In addition, this material failed induce neutralizing antibodies after immunization of rabbits. contrast, highly purified high-molecular-mass from supernatant H. cultures active a HeLa cell assay and effectively induced response Neutralizing sera were shown contain high proportion which...
Using computer modelling, we have identified some of the residues A subunit cholera toxin (CT) and heat-labile that are involved in NAD binding, catalysis, toxicity. Here describe site-directed mutagenesis CT gene construction mutants. Nine mutations were generated. Six them encoded proteins fully assembled AB5 structure nontoxic; these CT-D53 (Val-53-->Asp), CT-K63 (Ser-63-->Lys), CT-K97 (Val-97-->Lys), CT-K104 (Tyr-104-->Lys), CT-S106 (Pro-106-->Ser), double mutant...
Short stature due to SHOX deficiency represents a common congenital form of growth failure and is involved in the aetiology 'idiopathic' short deficits skeletal anomalies Leri–Weill, Langer Turner syndromes. Although much known on clinical molecular aspects haploinsufficiency, integration signalling pathways regulating bone currently not defined. Here we identify NPPB encoding natriuretic peptide, BNP, well-known cardiac peptide hormone, as transcriptional target SHOX. The ability...
Abstract H-1 parvovirus (H-1PV) is a promising anticancer therapy. However, in-depth understanding of its life cycle, including the host cell factors needed for infectivity and oncolysis, lacking. This may guide rational design combination strategies, aid development more effective viruses, help identify biomarkers susceptibility to H-1PV treatment. To involved, we carry out siRNA library screening using druggable genome library. We one crucial modulator infection: laminin γ1 ( LAMC1 )....
The aim of this study was to develop a RT-PCR assay for the specific detection SARS-CoV-2 Omicron Variant Concern (VOC) as rapid alternative sequencing.
The emergence of new SARS-CoV-2 variants poses challenges to global surveillance efforts, necessitating swift actions in their detection, evaluation, and management. Among the most recent variants, Omicron BA.2.86 its sub-lineages have gained attention due potential immune evasion properties. This study describes development a digital PCR assay for rapid detection descendant lineages, wastewater samples. By using this assay, we analyzed samples collected Italy from September 2023 January...
A novel protein family (p14.5, or YERO57c/YJGFc) highly conserved throughout evolution has recently been identified. The biological role of these proteins is not yet well characterized. Two members the p14.5 are present in yeast Saccharomyces cerevisiae. In this study, we have characterized some functions two proteins. Mmf1p a mitochondrial matrix factor, and homologous factor (Hmf1p) copurifies with soluble cytoplasmic fraction. Δmmf1cells lose DNA (mtDNA) decreased growth rate, while Δhmf1...
Regulation of gene expression is particularly important for dosage-dependent diseases and the phenomenon clinical heterogeneity frequently associated with these phenotypes. We here report on combined transcriptional translational regulatory mechanisms controlling Léri-Weill Turner syndrome SHOX. define an alternative promotor within exon 2 SHOX by transient transfections mono- bicistronic reporter constructs demonstrate substantial differences in translation efficiency mRNAs transcribed...