A5015297445- Neuropeptides and Animal Physiology
- Science, Research, and Medicine
- Receptor Mechanisms and Signaling
- Helicobacter pylori-related gastroenterology studies
- Cancer, Stress, Anesthesia, and Immune Response
- Cytokine Signaling Pathways and Interactions
- Peptidase Inhibition and Analysis
- Neuroscience and Neuropharmacology Research
- 3D Printing in Biomedical Research
- Biotechnology and Related Fields
- Inflammatory mediators and NSAID effects
- Single-cell and spatial transcriptomics
- Chemical Thermodynamics and Molecular Structure
- Pancreatitis Pathology and Treatment
- Synthesis and biological activity
- Eicosanoids and Hypertension Pharmacology
- Memory and Neural Mechanisms
- Hormonal Regulation and Hypertension
- Nitric Oxide and Endothelin Effects
- Cell Image Analysis Techniques
- Ion Transport and Channel Regulation
- Bioactive Compounds and Antitumor Agents
- Animal testing and alternatives
- Pharmacological Receptor Mechanisms and Effects
- Blood disorders and treatments
Istituto Nazionale di Ricerca Metrologica
2009-2024
Villa Melitta
1998-1999
Prous Institute for Biomedical Research (Spain)
1999
University of Milan
1983
The resazurin assay, also known as the Alamar Blue stands a cornerstone technique in cell biology, microbiology, and drug development. It assesses viability of cells through conversion into highly fluorescent resorufin. resulting fluorescence intensity provides reliable estimate viable numbers. Cytotoxicity assays, such resazurin-based method, play crucial role screening potential candidates assessment pharmaceutical chemical toxicity. In recent years, inconsistencies have arisen...
Main text Several scientific fields, such as cytology, biochemistry, molecular biology and genetics have cells their direct topic of study. The cell is a fundamental unit highly technological systems, employed, for example, in the development new chemicals drugs, evaluation compound toxicity, novel approaches to health, regenerative medicine. High content screening technologies are cellular imaging-based assays. In this framework, density, confluency size measurands that can describe...
Main text Nucleic acid amplification tests for SARS-CoV-2, the virus responsible COVID-19 pandemic, primarily target RNA as analyte. These detect presence of SARS-CoV-2 specific sequences, confirming infection through in vitro diagnostic methods. However, lack a standardized reference measurement system has led to varied units and unclear traceability reporting content quantities, complicating comparisons between different [1-5]. To address this challenge, pilot study CCQM-P199b was...
New (R)-4-benzamido-5-oxopentanoic acid derivatives were synthesized by a stereoconservative procedure and evaluated in vitro for their capacity to inhibit the binding of [125I](BH)-CCK-8 either rat peripheral (CCK-A) or central (CCK-B) CCK receptors, [3H]pentagastrin rabbit gastric glands, as well inhibit, vivo, secretion induced pentagastrin infusion perfused stomach. The parent compound this series (lorglumide) is first nonpeptidic, potent selective antagonist CCK-A receptor. Chemical...
A surface-labeled lyophilized lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells prelabeled with a fluorescein isothiocyanate conjugated anti-CD4 monoclonal antibody. The sLL is intended to be used as reference material for CD4+ cell counting including the development of higher order measurement procedures and evaluated in pilot study CCQM-P102. This was conducted across 16 laboratories from eight countries assess ability participants quantify...
ABSTRACT Nucleic acid amplification tests including reverse transcription quantitative PCR (RT-qPCR) are used to detect RNA from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the causative agent of disease 2019 (COVID-19) pandemic. Standardized measurements can facilitate comparable performance laboratory in absence existing reference measurement systems early on a Interlaboratory study CCQM P199b “SARS-CoV-2 copy number quantification” was designed test fitness-for-purpose...
The A549 cell line has become a cornerstone in biomedical research, particularly cancer studies and serves as critical tool cytotoxicity drug screening where it is used to evaluate the impact of pharmaceutical compounds on cellular viability. One most widely adopted methods for viability assessment, which also evaluating cytotoxicity, resazurin-based assay. This assay exploits ability living cells convert resazurin into fluorescent resorufin, providing reliable indicator metabolic activity....
This report focuses on the characterization of CD4 expression level in terms equivalent number reference fluorophores (ERF). Twelve different flow cytometer platforms across sixteen laboratories were utilized this study. As a first step participants asked to calibrate fluorescein isothiocyanate (FITC) channel each using commercially available calibration standard consisting five populations microspheres. Each population had an assigned value (EFF denotes special case generic term ERF with...
The effects of S-4-amino-5-[4,4-dimethylcyclohexyl)amino]-5-oxopentanoic acid (CR 2249), a new entity selected from series glutamic derivatives, has been investigated in different paradigms for screening nootropics. CR 2249 ameliorated the memory retention deficit produced by scopolamine step-through-type passive avoidance rats and electroconvulsive shock step-down-type mice. was also capable improving performance behavioural tests learning absence cholinergic hypofunction or cognitive...
This annual article presents new drugs and biologics that were launched or approved for the first time during previous year. In 2008, 31 medicines-this figure includes both therapeutic use as well diagnostic agents-reached their markets. Line extensions (new indications, formulations combinations of previously marketed products) accounted more than one-third medicines in 2008. addition to providing an overview all ever year, this will also review further depth first-in-class last a better...