A5052997771- Identification and Quantification in Food
- Biosensors and Analytical Detection
- Advanced Chemical Sensor Technologies
- Molecular Biology Techniques and Applications
- Gut microbiota and health
- Aquaculture disease management and microbiota
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Animal Virus Infections Studies
- Environmental DNA in Biodiversity Studies
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Virus-based gene therapy research
- Medicinal Plants and Neuroprotection
- Aquaculture Nutrition and Growth
- Single-cell and spatial transcriptomics
- Transgenic Plants and Applications
- interferon and immune responses
- Genetically Modified Organisms Research
- SARS-CoV-2 and COVID-19 Research
- Reproductive biology and impacts on aquatic species
- Gene expression and cancer classification
National Institute of Metrology
2022
National Physical Laboratory
2021
Main text Nucleic acid amplification tests for SARS-CoV-2, the virus responsible COVID-19 pandemic, primarily target RNA as analyte. These detect presence of SARS-CoV-2 specific sequences, confirming infection through in vitro diagnostic methods. However, lack a standardized reference measurement system has led to varied units and unclear traceability reporting content quantities, complicating comparisons between different [1-5]. To address this challenge, pilot study CCQM-P199b was...
Abstract Despite the advent of whole genome metagenomics, targeted approaches (such as 16S rRNA gene amplicon sequencing) continue to be valuable for determining microbial composition samples. Amplicon microbiome sequencing can performed on clinical samples from a normally sterile site determine aetiology an infection (usually single pathogen identification) or more complex niches such human mucosa environmental where multiple microorganisms need identified. The methodologies are frequently...
Immersion vaccination with a biomimetic mucoadhesive nanovaccine has been shown to induce strong mucosal immune response against columnaris disease, serious bacterial disease in farmed red tilapia caused by Flavobacterium columnare. However, the induction of systemic vaccine is yet be investigated. Here, we examine if specific humoral stimulated biomimetic-mucoadhesive columnare using an indirect-enzyme-linked immunosorbent assay (ELISA), serum bactericidal activity (SBA) and expression...
Circulating cell-free DNA (cfDNA) has attracted attention as a non-invasive biomarker for diagnosing and monitoring various cancers. Given that human papillomavirus (HPV) integration overexpression of E6/E7 oncogenes are pivotal events carcinogenesis, we sought to determine if HPV E7 cfDNA could serve specific cervical cancer detection. We applied droplet digital PCR (ddPCR) quantify HPV16/18 from the serum patients with cancer, intraepithelial neoplasia, controls. was highly displaying...
Circulating lncRNAs have attracted considerable attention as potential noninvasive biomarkers for diagnosing cancers. RT-qPCR is the canonical technique detecting circulating RNA and depends largely on stable reference genes data normalization. However, no systematic evaluation of serum lncRNA has been reported cervical cancer. Here, we profiled validated expression from cancer patients controls using microarrays RT-qPCR. We identified RP11-204K16.1, XLOC_012542, U6 small nuclear most based...
The use of the gastrointestinal tract as a site for local delivery DNA is an exciting prospect. In order to obtain effective vector capable delivering gene interest target cells achieve sufficient and sustained transgene expression, with minimal toxicity, we developed new generation filamentous bacteriophage. This particular bacteriophage was genetically engineered display arginine-glycine-aspartic acid (RGD) motif (an integrin-binding peptide) on major coat protein pVIII carry mammalian...
The application of high-throughput sequencing in profiling microbial communities is providing an unprecedented ability to investigate microbiomes. Such studies typically apply one two methods: amplicon using PCR target a conserved orthologous sequence (typically the 16S ribosomal RNA gene) or whole (meta)genome (WGS). Both methods have been used catalog taxa present sample and quantify their respective abundances. However, comparison inherent precision bias different approaches has not...
Abstract Pork adulteration is a major concern for Muslims and Jews whose diets are restricted by religious beliefs, as well those who allergic to pork meat its derivatives. Accurate authentication of great importance assist this demographic group people in making decision on their product purchase. The aim study was develop new analytical method processed products based combination loop-mediated isothermal amplification (LAMP) AuNP-nanoprobe colourimetric assay. LAMP conditions were first...
ABSTRACT Nucleic acid amplification tests including reverse transcription quantitative PCR (RT-qPCR) are used to detect RNA from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the causative agent of disease 2019 (COVID-19) pandemic. Standardized measurements can facilitate comparable performance laboratory in absence existing reference measurement systems early on a Interlaboratory study CCQM P199b “SARS-CoV-2 copy number quantification” was designed test fitness-for-purpose...
ABSTRACT Infection with human immunodeficiency virus (HIV)-1 leads to acquired syndrome (AIDS) if left untreated. According UN figures, approximately 39 million people globally were living HIV in 2022, 76% of those individuals accessing antiretroviral therapy. Measurement plasma viral RNA load using calibrated nucleic acid amplification tests (like reverse transcription quantitative PCR, RT-qPCR) is routinely performed monitor response treatment and ultimately prevent transmission....
Summary The alarming problem of meat adulteration emphasises the demand for accessible analytical approaches food regulatory agencies to detect and, specially, measure altered fractions. This study proposes a novel cross‐species triplex droplet digital polymerase chain reaction (ddPCR) assay simultaneously identify and quantify ratios pork/beef fractions from total DNA content, including processed autoclaved meat, without requiring standard, achieving high sensitivity with limit...
ABSTRACT For the impact of genomic testing from liquid biopsies to be maximized, mechanisms ensure reproducible and comparable test performance will required. This can established maintained through reference measurement procedures materials with property values that are internationally traceability a common standard. To achieve this objective, an interlaboratory study was organised explore digital PCR (dPCR) for standardisation cell-free DNA (cfDNA) quantification. Blinded samples...
Abstract In humans, peripheral blood cytokeratin 19 (CK19) mRNA-positive circulating tumor cells (CTCs) was utilized to identify early-stage breast cancer patients with micrometastatic disease who are at risk for progression and monitor treatment response in advanced disease. To our knowledge, there has been little research regarding CK19 canine mammary tumors (CMTs) using molecular methods. A droplet digital PCR (ddPCR) is proposed as a precise sensitive quantification of nucleic acid...
Abstract The problem in meat adulteration and food fraud emphasised the requirement of developing accurate analytical approaches for quantitative detection helping control adulteration. In this study, droplet digital Polymerase Chain Reaction (ddPCR) assays to quantify ratios pork DNA total amount were developed by challenging against extracted from a range gravimetrically prepared matrices beef. A single copy nuclear gene, β-actin , was employed as target accompanied with myostatin gene...
Funder: Quadram Institute Bioscience BBSRC Strategic Programme: Microbes in the Food Chain; Grant(s): BB/R012504/1) and its constituent projects BBS/E/F/000PR10348 BBS/E/F/000PR10349
Main text Breast cancer is the most common malignant tumor in women all over world. cancers with amplification of human epidermal growth factor receptor 2 (HER2) gene (referred to as HER2-positive breast cancer) accounts for about 20% ~ 30% invasive cancer, and this type characterized by high invasiveness, risk recurrence, rapid progression poor prognosis. The determination HER2 expression or copy number variant (CNV) (HER2 normalized cell a reference gene) cells patients beneficial choice...
Abstract Pork adulteration is a major concern for Muslims and Jews whose diets are restricted by religious beliefs, as well those who allergic to pork meat its derivatives. Accurate authentication of great importance assist this demographic group people in making decision on their product purchase. The aim study was develop new analytical method processed-meat products based combination loop-mediated isothermal amplification (LAMP) AuNP-nanoprobe colourimetric assay. LAMP conditions were...
Porcine epidemic diarrhea virus (PEDV) has been affecting the swine industry, especially in suckling pigs with a high mortality rate. Among all strategies to overcome PEDV, boosting mucosal immunity pig intestines through oral administration appears be more efficient than other routes. However, there are biological obstacles such as an acidic environment that could damage biologics, product from organisms often used for PEDV treatment. The plant-derived 2C10 monoclonal antibody (mAb)...