A5028055831- Microbial Metabolic Engineering and Bioproduction
- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- Cancer Treatment and Pharmacology
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Identification and Quantification in Food
- Biochemical Acid Research Studies
- Fungal and yeast genetics research
- Probiotics and Fermented Foods
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Facility Location and Emergency Management
- Microbial Metabolites in Food Biotechnology
- Advanced Chemical Sensor Technologies
- Viral Infectious Diseases and Gene Expression in Insects
- Fungal Biology and Applications
- SARS-CoV-2 detection and testing
- Regional Socio-Economic Development Trends
- Plant tissue culture and regeneration
- Microbial metabolism and enzyme function
- Biochemical and biochemical processes
- Environmental DNA in Biodiversity Studies
- Synthetic Organic Chemistry Methods
- SARS-CoV-2 and COVID-19 Research
University of Edinburgh
2017-2024
University College London
2010-2024
Biochemical Society
2014-2024
Newcastle University
2022-2023
Joint BioEnergy Institute
2016-2017
Lawrence Berkeley National Laboratory
2017
Universidad Autónoma del Estado de Morelos
2015
UCL Australia
2013
Abstract Background Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol ® ), remains limited despite growing global demands. Low yields critical taxadiene precursor a key bottleneck in microbial production. In this study, challenge poor synthase (TASY) solubility S. cerevisiae was revealed, and strains were strategically engineered to relieve bottleneck. Results Multi-copy chromosomal integration TASY harbouring selection fusion tags improved titres 22-fold,...
Simple and effective molecular diagnostic methods have gained importance due to the devastating effects of COVID-19 pandemic. Various isothermal one-pot detection been proposed as favorable alternatives standard RT-qPCR they do not require sophisticated and/or expensive devices. However, reactions are highly complex with a large number variables, determining optimum conditions maximize sensitivity while minimizing cost can be cumbersome. Here, statistical design experiments (DoE) was...
Science & Society26 April 2020Open Access The long journey towards standards for engineering biosystems Are the Molecular Biology and Biotech communities ready to standardise? Jacob Beal Raytheon BBN Technologies, Cambridge, MA, USA Search more papers by this author Angel Goñi-Moreno School of Computing, Newcastle University, upon Tyne, UK Centro de Biotecnología y Genómica Plantas, (CBGP, UPM-INIA), Universidad Politécnica Madrid, Pozuelo Alarcón, Spain Chris Myers...
Transketolase mutants previously identified for use with the non-phosphorylated aldehyde propanal have been explored a series of linear and cyclic aliphatic aldehydes, excellent stereoselectivities observed.
Taxadien-5α-hydroxylase and taxadien-5α-ol O-acetyltransferase catalyze the oxidation of taxadiene to subsequent acetylation taxadien-5α-yl-acetate in biosynthesis blockbuster anticancer drug, paclitaxel (Taxol®). Despite decades research, promiscuous multispecific CYP725A4 enzyme remains a major bottleneck microbial biosynthetic pathway development. In this study, an interdisciplinary approach was applied for construction optimization early Saccharomyces cerevisiae, across range bioreactor...
In this study, organic acids were demonstrated as a promising carbon source for bisabolene production by the non-conventional yeast, Rhodosporidium toruloides, at microscale with maximum titre of 1055 ± 7 mg/L. A 125-fold scale-up optimal process, enhanced titres 2.5-fold to 2606 Implementation pH controlled acid feeding strategy scale lead further threefold improvement in 7758 mg/L, highest reported microbial titre. Finally, proof-of-concept sequential bioreactor approach was investigated....
Integrated metabolic engineering approaches that combine system and synthetic biology tools enable the efficient design of microbial cell factories for synthesizing high-value products. In this study, we utilized in silico algorithms on yeast genome-scale model to predict genomic modifications could enhance production early-step Taxol
The recent technological advancements in synthetic biology have demonstrated the extensive potential socio-economic benefits at laboratory scale. However, translations of such technologies to industrial scale fermentations remains a major bottleneck. existence and lack understanding discrepancies cultivation conditions between scales often leads selection suboptimal bioprocessing conditions, crippling productivity. In this study, strategic design experiments approaches were coupled with...
Advances in synthetic biology are facilitating the de novo design of complex, multi-step enzymatic conversions for industrial organic synthesis. This work describes integration pathway construction with enzyme kinetics and bioreactor modelling, order to optimise synthesis chiral amino-alcohols using engineered Escherichia coli transketolases (TK) Chromobacterium violaceum transaminase (TAm). The specific target products were (2S,3S)-2-aminopentane-1,3-diol (APD)...
Abstract In situ product recovery is an efficient way to intensify bioprocesses as it can perform adsorption of the desired natural products in cultivation. However, common use only one adsorbent (liquid or solid) recovery. For this study, method with three combined commercial resins (HP‐20, XAD7HP, and HP‐2MG) different chemical properties was performed. A new yeast strain Saccharomyces cerevisiae engineered using CRISPR Cas9 (strain EJ2 ) deliver heterologous expression oxygenated...