A5018440823- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Biochemical Acid Research Studies
- Bacteriophages and microbial interactions
- Microbial Natural Products and Biosynthesis
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Enzyme Structure and Function
- Protein purification and stability
- Chemical Synthesis and Analysis
- Biofuel production and bioconversion
- Berberine and alkaloids research
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- Planetary Science and Exploration
- Chemical synthesis and alkaloids
- Biochemical and Molecular Research
- CRISPR and Genetic Engineering
- Synthesis and Biological Activity
- Antibiotic Resistance in Bacteria
- Virus-based gene therapy research
- Catalysis for Biomass Conversion
- Education, sociology, and vocational training
- Amino Acid Enzymes and Metabolism
University College London
2016-2025
Biochemical Society
2015-2024
UCL Australia
1993-2024
Wrap (United Kingdom)
2022
John Wiley & Sons (United States)
2016-2019
Hudson Institute
2016-2019
John Wiley & Sons (United Kingdom)
2019
Transnational Press London
2014-2018
Norwich Research Park
2017
University College Lahore
2017
A cyclophilin-D affinity matrix was employed to isolate components of the mitochondrial permeability transition pore. cDNA encoding cloned from a rat liver library and ligated into pGEX allow expression glutathione S-transferase/cyclophilin-D fusion protein in Escherichia coli XL1 cells. The functionally normal as judged by its peptidylprolyl cis-trans-isomerase activity inhibition cyclosporin A. bound glutathione-agarose form matrix. selectively 32-kDa proteins membrane extracts, but no...
The damaging effect of ionising radiation on cellular structure is one the prime limiting factors survival life in potential astrobiological habitats. Here we model propagation solar energetic protons and galactic cosmic ray particles through Martian atmosphere three different surface scenarios: dry regolith, water ice, regolith with layered permafrost. Particle energy spectra absorbed dose are determined for at regular depths underground, allowing calculation microbial times. Bacteria or...
Methodology which allows consistent shotgun cloning of streptomycete genes is presented. Parameters that increase transformation efficiency Streptomyces lividans 66 were adjusted to generate reproducibly a population cloned likely represent the entire genome. Factors influence recovery viable transformants include: growth phase mycelium, ionic and osmotic characteristics medium during protoplast formation transformation, moisture content density regeneration. A modified procedure was devised...
Two steps in one pot: An enzyme cascade consisting of a lyase and an (R)- or (S)-selective ω-transaminase (TA) provides (1R,2R)-norpseudoephedrine (1R,2S)-norephedrine only two steps. The intermediate is not isolated this one-pot reaction the products are obtained high enantio- diastereomeric purity. Moreover, by-product from second can be recycled to serve as substrate for first reaction. As service our authors readers, journal supporting information supplied by authors. Such materials peer...
Chemiosmotic coupling is universal: practically all cells harness electrochemical proton gradients across membranes to drive ATP synthesis, powering biochemistry. Autotrophic cells, including phototrophs and chemolithotrophs, also use power carbon fixation directly. The universality of chemiosmotic suggests that it arose very early in evolution, but its origins are obscure. Alkaline hydrothermal systems sustain natural the thin inorganic barriers interconnected micropores within deep-sea...
The amino functionality gives important biological activity in pharmaceutical compounds. formation of chiral amines and acids can be accomplished by several chemical routes but enzymatic offers many advantages preparing compounds or amination fragile compared to stoichiometric catalytic transformations. Biocatalytic primarily use enzymes the transaminase class (also known as aminotransferases) which transfer function from a donor organic compound ketone aldehyde acceptor. Although since...
A biocatalytic approach using transaminases has been used for the generation of a range furfurylamines in good yields.
Abstract Background Gut microbes influence animal health and thus, are potential targets for interventions that slow aging. Live E. coli provides the nematode worm Caenorhabditis elegans with vital micronutrients, such as folates cannot be synthesized by animals. However, microbe also limits C. lifespan. Understanding these interactions may shed light on how intestinal mammalian Results Serendipitously, we isolated an mutant slows We identified disrupted gene to aroD , which is required...
During the coronavirus (COVID-19) pandemic, UK government mandated use of face masks in various public settings and recommended reusable to combat shortages medically graded single-use healthcare. To assist decision-making on choice for future pandemics, where may not be a contributing factor, University College London (UCL) Plastic Waste Innovation Hub has carried out multidisciplinary comparison between based their anatomy, standalone effectiveness, behavioural considerations,...
Abstract Chemoenzymatic and enzymatic cascade reactions enable the synthesis of complex stereocomplementary 1,3,4‐trisubstituted tetrahydroisoquinolines (THIQs) with three chiral centers in a step‐efficient selective manner without intermediate purification. The employs inexpensive substrates (3‐hydroxybenzaldehyde pyruvate), involves carboligation step, subsequent transamination, finally Pictet–Spengler reaction carbonyl cosubstrate. Appropriate selection carboligase transaminase enzymes...
ATP is universally conserved as the principal energy currency in cells, driving metabolism through phosphorylation and condensation reactions. Such deep conservation suggests that arose at an early stage of biochemical evolution. Yet purine synthesis requires 6 steps linked to hydrolysis. This autocatalytic requirement for synthesize implies need earlier prebiotic equivalent, which could drive protometabolism before synthesis. Why this phosphorylating agent was replaced, specifically with...
Heart and liver mitochondria contain a structure that is able to form large non‐selective pore in the inner membrane under conditions of high matrix Ca 2+ oxidant stress. The blocked by cyclo‐sporin A (CSA). In this study, rat were covalently labelled with photoactive CSA derivative presence absence ligands ADP Photolabelling 21‐kDa protein was selectively depressed manner reversed ADP. exhibited peptidyl‐prolyl cis‐trans isomerase (PPIase) activity inhibited (K i , 8 nM). PPIase associated...
Abstract Biocatalysis continues to emerge as a powerful technique for the efficient synthesis of optically pure pharmaceuticals that are difficult access via conventional chemistry. The power biocatalysis can be enhanced if two or more reactions achieved by single whole cell biocatalyst containing pathway designed de‐novo facilitate required synthetic sequence. enzymes transketolase (TK) and transaminase (TAm) respectively catalyze asymmetric carboncarbon bond formation amine group addition...