A5006389025- Weed Control and Herbicide Applications
- Glutathione Transferases and Polymorphisms
- Genomics, phytochemicals, and oxidative stress
- Pesticide and Herbicide Environmental Studies
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Allelopathy and phytotoxic interactions
- Phytochemicals and Antioxidant Activities
- Nitrogen and Sulfur Effects on Brassica
- Agriculture and Biological Studies
- Legume Nitrogen Fixing Symbiosis
- Childhood Cancer Survivors' Quality of Life
- Polyamine Metabolism and Applications
- Plant Molecular Biology Research
- Insect and Pesticide Research
- Plant-Microbe Interactions and Immunity
- Plant biochemistry and biosynthesis
- Diagnosis and Treatment of Venous Diseases
- Peripheral Artery Disease Management
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Soybean genetics and cultivation
- Plant and fungal interactions
- Family Support in Illness
Newcastle University
2016-2025
Royal Marsden NHS Foundation Trust
2005-2022
Wesley Hospital
2018-2020
Durham University
2005-2018
Royal Marsden Hospital
1999-2018
American Association of State Colleges and Universities
2016
American Institutes for Research
2016
SRI International
2016
University of York
2011-2014
Fera Science (United Kingdom)
2010-2014
Searches with the human Omega glutathione transferase (GST) identified two outlying groups of GST superfamily in <i>Arabidopsis thaliana</i> which differed from all other plant GSTs by containing a cysteine place serine at active site. One group consisted four genes, three encoded glutathione-dependent dehydroascorbate reductases (DHARs). Two DHARs were predicted to be cytosolic, whereas contained chloroplast targeting peptide. The also as thiol transferases but had no conjugating activity....
Multiple-herbicide resistance (MHR) in black-grass ( Alopecurus myosuroides ) and annual rye-grass Lolium rigidum is a global problem leading to loss of chemical weed control cereal crops. Although poorly understood, common with multiple-drug (MDR) tumors, MHR associated an enhanced ability detoxify xenobiotics. In humans, MDR linked the overexpression pi class glutathione transferase (GSTP1), which has both detoxification signaling functions promoting drug resistance. black-grass, was also...
Enzyme screens with Strep-tagged recombinant proteins and expression studies the respective green fluorescent protein (GFP) fusions have been employed to examine functional activities subcellular localization of members Arabidopsis glutathione transferase (GST) superfamily. Fifty-one 54 GST family were transcribed 41 found express as glutathione-dependent enzymes in Escherichia coli. Functional redundancy was observed particular three theta (T) class GSTs showed conserved...
Summary Black‐grass ( Alopecurus myosuroides ) is a major weed of wheat in Europe, with several populations having acquired resistance to multiple herbicides differing modes action. As compared herbicide‐susceptible black‐grass, showing herbicide cross‐resistance contained greatly elevated levels specific type I glutathione transferase (GST), termed Am GST2, but similar III GST GST1. Following cloning and expression the respective cDNAs, GST2 differed from GST1 limited activity detoxifying...
Biosynthesis of phenylpropanoid natural products in tobacco was perturbed by introduction a heterologous (bean) phenylalanine ammonia-lyase (PAL; L-phenylalanine ammonia-lyase, EC 4.3.1.5) gene, modified inclusion cauliflower mosaic virus 35S enhancer sequences its promoter. These transgenic plants can exhibit series unusual phenotypes including localized fluorescent lesions, altered leaf shape and texture, reduced signification xylem, stunted growth, pollen viability, flower morphology...
The glucosylation of pollutant and pesticide metabolites in plants controls their bioactivity the formation subsequent chemical residues. model plant Arabidopsis thaliana contains >100 glycosyltransferases (GTs) dedicated to small-molecule conjugation and, whereas 44 these enzymes catalyze O-glucosylation chlorinated phenols, only one, UGT72B1, shows appreciable N-glucosylating activity toward chloroanilines. UGT72B1 is a bifunctional O-glucosyltransferase (OGT) N-glucosyltransferase (NGT)....
Abstract S-Glutathionylation (thiolation) is a ubiquitous redox-sensitive and reversible modification of protein cysteinyl residues that can directly regulate their activity. While well established in animals, little known about the formation function these mixed disulfides plants. After labeling intracellular glutathione pool with [35S]cysteine, suspension cultures Arabidopsis (Arabidopsis thaliana ecotype Columbia) were shown to undergo large increase thiolation following treatment oxidant...
The 55 Arabidopsis glutathione transferases (GSTs) are, with one microsomal exception, a monophyletic group of soluble enzymes that can be divided into phi, tau, theta, zeta, lambda, dehydroascorbate reductase (DHAR) and TCHQD classes. populous phi tau classes are often highly stress inducible regularly crop up in proteomic transcriptomic studies. Despite much study on their xenobiotic-detoxifying activities natural roles unclear, although defence-related secondary metabolism likely. smaller...
To identify factors linked with emotional and behavioural problems in school age (6- to 17-year-old) children of women breast cancer. Reports children's were obtained from patient mothers, their healthy partners, the teacher adolescents using Child Behaviour Checklist Mental Health subscale Questionnaire. Parents reported on own level depression and, for patients only, quality life. Family functioning was assessed Assessment Device Cohesion Environment Scale. Using a cross-sectional within...
Summary Cultivars of purple tea ( Camellia sinensi s) that accumulate anthocyanins in place catechins are currently attracting global interest their use as functional health beverages. RNA‐seq normal (LJ43) and Zijuan (ZJ) cultivars identified the transcription factor CsMYB75 phi (F) class glutathione transferase CsGSTF1 being associated with anthocyanin hyperaccumulation. Both genes mapped a quantitative trait locus (QTL) to bud leaf color (BLC) F 1 populations, promoting expression...
Abstract Background Gut microbes influence animal health and thus, are potential targets for interventions that slow aging. Live E. coli provides the nematode worm Caenorhabditis elegans with vital micronutrients, such as folates cannot be synthesized by animals. However, microbe also limits C. lifespan. Understanding these interactions may shed light on how intestinal mammalian Results Serendipitously, we isolated an mutant slows We identified disrupted gene to aroD , which is required...
Abstract Intense selection by pesticides and antibiotics has resulted in a global epidemic of evolved resistance. In agriculture medicine, using mixtures compounds from different classes is widely accepted as optimal resistance management. However, this strategy may promote the evolution more generalist mechanisms. Here we test hypothesis at national scale an economically important agricultural weed: blackgrass ( Alopecurus myosuroides) , for which herbicide major economic issue. Our results...
Abstract Several grass and broadleaf weed species around the world have evolved multiple-herbicide resistance at alarmingly increasing rates. Research on biochemical molecular mechanisms of multiple-resistant populations indicate a prevalence herbicide metabolism catalyzed by enzyme systems such as cytochrome P450 monooxygenases glutathione S -transferases and, to lesser extent, glucosyl transferases. A symposium was conducted gain an understanding current state research metabolic in that...