A5006284440- Viral Infectious Diseases and Gene Expression in Insects
- GABA and Rice Research
- RNA and protein synthesis mechanisms
- Biofuel production and bioconversion
- Bioactive Natural Diterpenoids Research
- Plant biochemistry and biosynthesis
- Molecular Biology Techniques and Applications
- Phytochemistry and Biological Activities
- Microbial Metabolic Engineering and Bioproduction
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Microbial Natural Products and Biosynthesis
- Enzyme Catalysis and Immobilization
- Lipid metabolism and biosynthesis
- Microfluidic and Capillary Electrophoresis Applications
- Cannabis and Cannabinoid Research
- Microfluidic and Bio-sensing Technologies
- Biochemical and biochemical processes
- Medicinal Plant Extracts Effects
California Institute of Technology
2023-2024
Lawrence Berkeley National Laboratory
2017-2020
University of California, Berkeley
2017-2019
Joint BioEnergy Institute
2017-2018
Here, we present a method for expressing multiple open reading frames (ORFs) from single transcripts using the leaky scanning model of translation initiation. In this approach termed "stoichiometric expression mRNA polycistrons by eukaryotic ribosomes" (SEMPER), adjacent ORFs are translated at tunable ratios determined their order in sequence and strength initiation sites. We validate up to three fluorescent proteins one plasmid two different cell lines. then use it encode stoichiometrically...
ABSTRACT Applications of mammalian synthetic biology increasingly require the ability to express multiple proteins at user-determined stoichiometries from single, compactly encoded transcripts. Here we present an approach for expressing open reading frames (ORFs) a single transcript, taking advantage leaky scanning model translation initiation. In this method, adjacent ORFs are translated messenger RNA tunable ratios determined by their order in sequence and strength initiation sites. We...