A5010225578- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Acute Myeloid Leukemia Research
- Genetic Associations and Epidemiology
- Nutrition, Genetics, and Disease
- HIV/AIDS drug development and treatment
- Cancer-related gene regulation
- Monoclonal and Polyclonal Antibodies Research
- Bioinformatics and Genomic Networks
- RNA Research and Splicing
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Advanced biosensing and bioanalysis techniques
- Protein Degradation and Inhibitors
- Click Chemistry and Applications
- Advanced Biosensing Techniques and Applications
- Genomics and Rare Diseases
- GABA and Rice Research
- Nanoparticle-Based Drug Delivery
- Pneumocystis jirovecii pneumonia detection and treatment
- Cannabis and Cannabinoid Research
- Immune Cell Function and Interaction
- Gene expression and cancer classification
- CRISPR and Genetic Engineering
- Chronic Lymphocytic Leukemia Research
University of Chicago
2014-2024
QB3
2019-2020
University of California, Berkeley
2019-2020
Jagiellonian University
2010
Virginia Commonwealth University
2010
The blood proteome holds great promise for precision medicine but poses substantial challenges due to the low abundance of most plasma proteins and vast dynamic range proteome. Here we address these with NUcleic acid Linked Immuno-Sandwich Assay (NULISA™), which improves sensitivity traditional proximity ligation assays by ~10,000-fold attomolar level, suppressing assay background via a dual capture release mechanism built into oligonucleotide-conjugated antibodies. Highly multiplexed...
Abstract By examination of the cancer genomics database, we identified a new set mutations in core histones that frequently recur patient samples and are predicted to disrupt nucleosome stability. In support this idea, characterized glutamate lysine mutation histone H2B at amino acid 76 (H2B-E76K), found particularly bladder head neck cancers, disrupts interaction between H4. Although H2B-E76K forms dimers with H2A, it does not form stable octamers H3 H4 vitro, when reconstituted DNA...
Abstract Deep sequencing has revealed that epigenetic modifiers are the most mutated genes in acute myeloid leukemia (AML). Thus, elucidating dysregulation AML is crucial to understand disease mechanisms. Here, we demonstrate metal response element binding transcription factor 2/polycomblike 2 (MTF2/PCL2) plays a fundamental role polycomb repressive complex (PRC2) and its loss elicits an altered state underlying refractory AML. Unbiased systems analyses identified of MTF2–PRC2 repression...
Significance Extensive studies of the structure–function relationship antibodies have established that conventional immunoglobulins contain two copies antigen-binding fragment (Fab), each which serves as an autonomous and complete unit for recognizing antigen. In this paper, we report a previously unidentified mode antibody–antigen recognition, dubbed “antigen clasping,” where sites cooperatively clasp one antigen, design long-neck antibody format facilitates antigen clasping. Antigen...
Abstract Sperm contributes genetic and epigenetic information to the embryo efficiently support development. However, mechanism underlying such developmental competence remains elusive. Here, we investigated whether all sperm cells have a common configuration that primes transcriptional program for embryonic Using calibrated ChIP-seq, show remodelling of histones during spermiogenesis results in retention methylated histone H3 at same genomic location most cell. This homogeneously fraction...
Transcription factors of the nuclear factor 1 (NFI) family regulate normal brain development in vertebrates. However, multiple splice variants four NFI isoforms exist, and their biological functions have yet to be elucidated. Here, we cloned analyzed human NFI-X3, a novel variant nfix gene, which contains unique transcriptional activation (TA) domain completely conserved primates. In contrast previously NFI-X1, overexpression NFI-X3 potently activates reporters, including glial fibrillary...
Abstract Considerable mechanistic insight into the function of histone post‐translational modifications and enzymes that install remove them derives from in vitro experiments with modified histones, often embedded nucleosomes. We report first semisyntheses native‐like 3 (H3) bearing tri‐ dimethyllysines at position 79 trimethyllysine 36, as well more facile traceless K9 K27 trimethylated species. These are practical on a multi‐milligram scale can also generate H3 combinations marks. Each...
The blood proteome holds great promise for precision medicine but poses substantial challenges due to the low abundance of most plasma proteins and vast dynamic range across proteome. We report a novel proteomic technology - NUcleic acid Linked Immuno-Sandwich Assay (NULISA™) that incorporates dual capture release mechanism suppress assay background improves sensitivity proximity ligation by over 10,000-fold attomolar level. It utilizes pairs antibodies conjugated DNA oligonucleotides enable...
Expression of vast repertoires antigen receptors by lymphocytes, with each cell expressing a single receptor, requires stochastic activation individual variable (V) genes for transcription and recombination. How this occurs remains unknown. Using single-cell RNA sequencing (scRNA-seq) allelic variation, we show that pre-B cells monoallelically transcribe divergent arrays Vκ genes, thereby opening subsequent Vκ-Jκ Transcription upon translocation to polymerase II arrayed on the nuclear matrix...
Abstract Nucleosomes, composed of DNA and histone proteins, represent the fundamental repeating unit eukaryotic genome 1 ; posttranslational modifications these proteins influence activity associated genomic regions to regulate cell identity 2–4 . Traditionally, trimethylation 3-lysine 4 (H3K4me3) is with transcriptional initiation 5–10 , whereas H3K27 (H3K27me3) considered transcriptionally repressive 11–15 The apparent juxtaposition opposing marks, termed “bivalent domains” 16–18 was...
<p>Supplemental materials and methods Supplemental Figures 1-7</p>