A5045178955- Monoclonal and Polyclonal Antibodies Research
- Lymphoma Diagnosis and Treatment
- HER2/EGFR in Cancer Research
- Free Radicals and Antioxidants
- Electrochemical Analysis and Applications
- Advanced oxidation water treatment
- Photochemistry and Electron Transfer Studies
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Medicinal Plants and Neuroprotection
- Natural Antidiabetic Agents Studies
- Radical Photochemical Reactions
- Radiation Effects and Dosimetry
- Medicinal Plant Research
- Genomic variations and chromosomal abnormalities
- Electron Spin Resonance Studies
- Cancer Cells and Metastasis
- Mass Spectrometry Techniques and Applications
- bioluminescence and chemiluminescence research
- Spectroscopy and Quantum Chemical Studies
- Foreign Body Medical Cases
- Oxidative Organic Chemistry Reactions
- CRISPR and Genetic Engineering
- Genetic Syndromes and Imprinting
- Cardiac Ischemia and Reperfusion
University of Cambridge
2017-2024
Cancer Research UK Cambridge Center
2017-2024
M.J.P. Rohilkhand University
2015-2024
Cancer Research UK
2018-2024
Yashoda Hospital
2024
National Institute of Disaster Management
2023
Chandigarh University
2022
Army Hospital Research and Referral
2022
Center for Genomic Science
2015-2020
Italian Institute of Technology
2015-2020
Diabetic nephropathy (DN), a microvascular complication of diabetes, has emerged as an important health problem worldwide. There is strong evidence to suggest that oxidative stress, inflammation, and fibrosis play pivotal role in the progression DN. Apigenin been shown possess antioxidant, anti-inflammatory, antiapoptotic, antifibrotic, well antidiabetic properties. Hence, we evaluated whether apigenin halts development DN streptozotocin (STZ)-induced diabetic rats. Male albino Wistar rats...
Chromosomal rearrangements occur constitutionally in the general population and somatically majority of cancers. Detection balanced rearrangements, such as reciprocal translocations inversions, is troublesome, which particularly detrimental oncology where play diagnostic prognostic roles. Here we describe use Hi-C a tool for detection both unbalanced chromosomal primary human tumour samples, with potential to define chromosome breakpoints bp resolution. In addition, show copy number profiles...
Abstract Understanding the dynamics of endogenous protein–protein interactions in complex networks is pivotal deciphering disease mechanisms. To enable in-depth analysis protein chromatin-associated complexes, we have previously developed a method termed RIME (Rapid Immunoprecipitation Mass spectrometry Endogenous proteins). Here, present quantitative multiplexed (qPLEX-RIME), which integrates with isobaric labelling and tribrid mass for study interactome fashion increased sensitivity. Using...
Numerous methods are available to profile several epigenetic marks, providing data with different genome coverage and resolution. Large epigenomic datasets then generated, often combined other high-throughput data, including RNA-seq, ChIP-seq for transcription factors (TFs) binding DNase-seq experiments. Despite the numerous computational tools covering specific steps in analysis of large-scale epigenomics comprehensive software solutions their integrative still missing. Multiple must be...
The global epidemic of type 2 diabetes demands the rapid evaluation new and accessible interventions. This study investigated whether Aegle marmelos fruit aqueous extract (AMF; 250, 500 1000 mg/kg) improves insulin resistance, dyslipidemia β‐cell dysfunction in high fat diet fed‐streptozotocin (HFD‐STZ)‐induced diabetic rats by modulating peroxisome proliferator‐activated receptor‐γ (PPARγ) expression. serum levels glucose, insulin, homeostasis model assessment resistance (HOMA‐IR), function...
Next-generation sequencing (NGS) technologies have deeply changed our understanding of cellular processes by delivering an astonishing amount data at affordable prices; nowadays, many biology laboratories already accumulated a large number sequenced samples. However, managing and analyzing these poses new challenges, which may easily be underestimated research groups devoid IT quantitative skills. In this perspective, we identify five issues that should carefully addressed approaching NGS...
Centrosomes are the major microtubule-organizing centers in animals and play fundamental roles many cellular processes. Understanding how their composition varies across diverse cell types it is altered disease unresolved questions, yet currently available centrosome isolation protocols cumbersome time-consuming, they lack scalability. Here, we report development of affinity capture (CAPture)-mass spectrometry (MS), a powerful one-step purification method to obtain high-resolution proteomes...
Abstract Polatuzumab vedotin (Pola-V) is an antibody–drug conjugate directed to the CD79B subunit of B-cell receptor (BCR). When combined with conventional immunochemotherapy, Pola-V improves outcomes in diffuse large lymphoma (DLBCL). To identify determinants sensitivity, we used CRISPR-Cas9 screening for genes that modulated toxicity lymphomas or surface expression its target, CD79B. Our results reveal striking impact glycosylation on epitope availability cell and toxicity. Genetic,...
Abstract Sperm contributes genetic and epigenetic information to the embryo efficiently support development. However, mechanism underlying such developmental competence remains elusive. Here, we investigated whether all sperm cells have a common configuration that primes transcriptional program for embryonic Using calibrated ChIP-seq, show remodelling of histones during spermiogenesis results in retention methylated histone H3 at same genomic location most cell. This homogeneously fraction...
Estrogen receptor-α (ER) drives tumor development in ER-positive (ER+) breast cancer. The transcription factor GATA3 has been closely linked to ER function, but its precise role this setting remains unclear. Quantitative proteomics was used assess changes the complex response depletion. Unexpectedly, few proteins were lost from absence of GATA3, with only major change being depletion dioxygenase TET2. TET2 binding constituted a near-total subset multiple cancer models, loss associated...
EHMT1 (also known as GLP) is a multifunctional protein, best for its role an H3K9me1 and H3K9me2 methyltransferase through reportedly obligatory dimerization with EHMT2 G9A). Here, we investigated the of in oocyte comparison to using oocyte-specific conditional knockout mouse models ( Ehmt2 cKO, Ehmt1 Ehmt1/2 cDKO), ablation from early phase growth. Loss cKO cDKO oocytes recapitulated meiotic defects observed cKO; however, there was significant impairment maturation developmental competence...
Abstract Accumulation of aggregated and misfolded proteins, leading to endoplasmic reticulum stress activation the unfolded protein response, is a hallmark several neurodegenerative disorders, including Alzheimer’s Parkinson’s disease. Genetic screens are powerful tools that proving invaluable in identifying novel modulators disease associated processes. Here, we performed loss-of-function genetic screen using human druggable genome library, followed by an arrayed-screen validation,...
Memory formation is necessary for the survival of animals across phyla. Here, we elucidate mechanism underlying long-term associative memory (LTAM) formed by treating Caenorhabditis elegans with a volatile chemoattractant and heat. Previous work has shown that training paradigm involving heat isoamyl alcohol (IAA) simultaneously, causes C. to lose their attraction IAA. In this study, elaborate on behind LTAM suggest during IAA, release extracellular vesicles (EVs) upon being taken up same...
To evaluate the capability of hyperpolarized [1-13C] pyruvate MRI to predict pathologic response neoadjuvant treatment in multi-site abdominopelvic disease high-grade serous ovarian cancer (HGSOC) patients and compare 13C [18F]-FDG PET/CT measurements for detecting early response. We recruited eight with HGSOC this prospective study who underwent before after first cycle chemotherapy (NACT). Imaging parameters were compared clinical histophatologic parameters. demonstrate here that...
Using the pulse radiolysis technique, studies on reactions of 3,4,5-trihydroxybenzoic acid [gallic (GA)] with radical species generated in water are reported. At pH 6.8 and 9.7, OH radicals react GA to give an adduct initially rate constants order 1×1010 d mol-1 s-1. This then reacts parent molecules 5×108 s-1 phenoxyl type having absorption maxima 350 nm region. 12 13.6, OH/O- directly bring about oxidation GA. Specific oxidants like azidyl one electron at neutral alkaline pHs almost...
Critical illness is accompanied by the release of large amounts anaphylotoxin, C5a. C5a suppresses antimicrobial functions neutrophils which associated with adverse outcomes. The signaling pathways that mediate C5a-induced neutrophil dysfunction are incompletely understood. Healthy donor exposed to purified demonstrated a prolonged defect (7 hours) in phagocytosis Staphylococcus aureus. Phosphoproteomic profiling 2712 phosphoproteins identified persistent and selective impairment phagosomal...
Abstract Th17 cells are key drivers of autoimmune disease. However, the signaling pathways regulating polarization poorly understood. Hedgehog regulates cell fate decisions during embryogenesis and adult tissue patterning. Here we find that cell-autonomous signaling, independent exogenous ligands, selectively drives but not other T helper subsets. We show endogenous ligand, Ihh, signals to activate both canonical non-canonical through Gli3 AMPK. demonstrate pathway inhibition with either...
Disaster risks are increasing and changing their nature. The global risk landscape is evolving as new – many of them arising from cascading inter-connected hazards arising. While significant achievements have been made to reduce loss lives, economic asset damages still on the trend. G20, which started major group meeting, has emerged over years an influential political forum. there several attempts bring disaster reduction agenda issue not addressed in a comprehensive sustained manner. A...
The inner ear is a complex structure responsible for hearing and balance, organ pathology associated with deafness balance disorders. To evaluate the role of epigenomic dynamics, we performed whole genome bisulfite sequencing at key time points during development maturation mouse sensory epithelium (SE). Our single-nucleotide resolution maps revealed variations in both general characteristics dynamics DNA methylation over time. This allowed us to predict location non-coding regulatory...