A5084887362- Lymphoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Acute Myeloid Leukemia Research
- Neutropenia and Cancer Infections
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- Chronic Myeloid Leukemia Treatments
- CRISPR and Genetic Engineering
- Hematopoietic Stem Cell Transplantation
- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Bacterial Identification and Susceptibility Testing
- CNS Lymphoma Diagnosis and Treatment
- Infections and bacterial resistance
- Advanced biosensing and bioanalysis techniques
- Antibiotic Resistance in Bacteria
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Protein Degradation and Inhibitors
- Biochemical and Molecular Research
- Multiple Myeloma Research and Treatments
- Viral-associated cancers and disorders
National Institutes of Health
2021-2024
National Cancer Institute
2021-2024
Frankfurt Cancer Institute
2024
Deutschen Konsortium für Translationale Krebsforschung
2024
University Hospital Frankfurt
2017-2024
Goethe University Frankfurt
2017-2024
Center for Cancer Research
2022-2024
Justus-Liebig-Universität Gießen
2018
Zero to Three
2017
Integrated analysis of genomes, transcriptomes, proteomes and drug responses cancer cell lines (CCLs) is an emerging approach to uncover molecular mechanisms action. We extend this paradigm measuring proteome activity landscapes by acquiring integrating quantitative data for 10,000 proteins 55,000 phosphorylation sites (p-sites) from 125 CCLs. These are used contextualize p-sites predict sensitivity. For example, we find that Progesterone Receptor (PGR) associated with sensitivity drugs...
Significance B cell development and function depend on BCR signaling. Infection of primary cells by EBV relies the EBV-encoded protein LMP2A, which is currently thought to mimic We revise this model based systematic comparison LMP2A signaling at phosphoproteome transcriptome levels. Our study identifies modules that are not shared elucidates their impact gene expression in cells. describe a mechanism interferes with apoptosis cell-cycle checkpoints cooperate oncogenes promoting survival...
Research Article10 August 2018Open Access Transparent process Comparative proteomics reveals a diagnostic signature for pulmonary head-and-neck cancer metastasis Hanibal Bohnenberger Institute of Pathology, University Medical Center, Göttingen, Germany Search more papers by this author Lars Kaderali Bioinformatics, Medicine Greifswald, Philipp Ströbel Diego Yepes Department II, Hematology/Oncology, Goethe University, Frankfurt, German Cancer Consortium, Heidelberg, Uwe Plessmann...
Clonal hematopoiesis of indeterminate potential (CHIP) is caused by recurrent somatic mutations leading to clonal blood cell expansion. However, direct evidence the fitness CHIP-mutated human hematopoietic stem cells (HSCs) in reconstitution lacking. Because myeloablative treatment and transplantation enforce stress on HSCs, we followed 81 patients with solid tumors or lymphoid diseases undergoing autologous (ASCT) for development CHIP. We found a high incidence CHIP (22%) after ASCT mean...
Abstract Hypomethylating agents decitabine and azacytidine are regarded as interchangeable in the treatment of acute myeloid leukemia (AML). However, their mechanisms action remain incompletely understood, predictive biomarkers for HMA efficacy lacking. Here, we show that bioactive metabolite triphosphate, but not functions activator substrate triphosphohydrolase SAMHD1 is subject to SAMHD1-mediated inactivation. Retrospective immunohistochemical analysis bone marrow specimens from AML...
Abstract Diffuse large B-cell lymphoma (DLBCL) can be subdivided into the activated (ABC) and germinal center B cell–like (GCB) subtypes. Self-antigen engagement of receptors (BCR) in ABC tumors induces their clustering, thereby initiating chronic active signaling activation NF-κB PI3 kinase. Constitutive BCR is essential some GCB but primarily activates We devised genome-wide CRISPR–Cas9 screens to identify regulators IRF4, a direct transcriptional target an indicator proximal DLBCL....
Functional genomics identifies N-acetyllactosamine extension of complex N-glycans
Abstract Polatuzumab vedotin (Pola-V) is an antibody–drug conjugate directed to the CD79B subunit of B-cell receptor (BCR). When combined with conventional immunochemotherapy, Pola-V improves outcomes in diffuse large lymphoma (DLBCL). To identify determinants sensitivity, we used CRISPR-Cas9 screening for genes that modulated toxicity lymphomas or surface expression its target, CD79B. Our results reveal striking impact glycosylation on epitope availability cell and toxicity. Genetic,...
BACKGROUND Allogeneic hematopoietic stem cell transplantation (allo‐HSCT) is a curative treatment option for patients with acute myeloid leukemia (AML). During transplantation, undergo period of severe neutropenia, which puts them at high risk infectious complications. However, the impact patient colonization multidrug‐resistant organisms (MDRO) on overall survival remains unclear. METHODS In this retrospective, single‐center study, authors analyzed data from 264 AML who underwent first...
A significant increase in infections caused by multidrug-resistant organisms (MDRO) has been observed recent years, resulting an of mortality all fields health care. Hematological patients are particularly affected MDRO because disease- and therapy-related immunosuppression. To determine the impact colonization with on overall survival, we retrospectively analyzed data from undergoing autologous hematopoietic stem cell transplantation at our institution. In total, 184 were identified, mainly...
Introduction Treatment of osteoporotic fractures is still challenging and an urgent need exists for new materials, better adapted to bone by adjusted Young's modulus, appropriate surface modification pharmaceuticals. Materials methods Titanium-40-niobium alloys, mechanically ground or additionally etched titanium-6-aluminium-4-vanadium were analyzed in combination with brain-derived neurotrophic factor, acetylcholine nicotine determine their effects on human mesenchymal stem cells vitro over...
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) offers potential cure to acute myeloid leukemia (AML) patients. However, infections with commensal bacteria are an important cause for non-relapse mortality (NRM). We have previously described the impact of multidrug-resistant organism (MDRO) colonization on survival allo-HSCT In aforementioned publication, according consensus, we there did not consider opportunistic gram-negative bacterium Stenotrophomonas maltophilia (S....
Abstract Tissue-specific differences in the expression of paralog genes, which are not essential most cell types due to buffering effect partner pair, can make for highly selective gene dependencies. To identify paralogous targets acute myeloid leukemia (AML), we integrated Cancer Dependency Map with numerous datasets characterizing protein–protein interactions, relationships, and cancer models. In this study, identified ATP1B3 as a context-specific, paralog-related dependency AML. ATP1B3,...
Abstract Bloodstream infections (BSI) are a frequent complication in patients with hematological and oncological diseases. However, the impact of different bacterial species causing BSI multiple remains incompletely understood. We performed retrospective study profiling 637 episodes patients. Based on 30-day (30d) overall survival (OS), we analyzed types grouped BSI-associated bacteria into clusters followed by further assessment clinical infection-related characteristics. discovered that...
Autologous hematopoietic stem cell transplantation (auto-HSCT) provides a potentially curative treatment option for relapsed and refractory lymphomas. Obesity displays an emerging epidemic risk factor global mortality is associated with increased in cancer patients. To date, the impact of obesity on outcome lymphoma patients undergoing auto-HSCT understudied. We conducted retrospective single-center study assessing 119 who underwent auto-HSCT. Overall survival (OS) served as primary endpoint...
ObjectivesClostridioides difficile infections (CDI) are common in autologous (auto-HSCT) or allogenic hematopoietic stem cell transplant (allo-HSCT) recipients. However, the impact of CDI on patient outcomes is controversial. We conducted this study to examine outcomes.MethodsWe performed a retrospective single-center study, including 191 lymphoma patients receiving an auto-HSCT and 276 acute myeloid leukemia (AML) allo-HSCT. The primary endpoint was overall survival (OS). Secondary...
Abstract Background Acute myeloid leukaemia (AML) is treated with intensive induction chemotherapy (IT) in medically fit patients. In general, obesity was identified as a risk factor for all-cause mortality, and there an ongoing debate on its impact outcome optimal dosing strategy obese AML Methods We conducted registry study screening 7632 patients assessed the of 1677 equally IT treated, newly diagnosed (OS, EFS, CR1), comorbidities, toxicities used strategies. Results Obese (BMI ≥ 30)...
Abstract Prednisone is an anti-inflammatory glucocorticoid (GC) that cytotoxic for normal and malignant B cells and, on this basis, has long been included in combination chemotherapies to treat aggressive B-cell lymphomas. However, the mechanisms by which GCs kill lymphoma still largely remains elusive due pleiotropic consequences of GC binding receptor (GR; NR3C1), a ligand-induced transcription factor. This prompted us search critical direct targets action with hypothesis may inhibit key...