A5089006758- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Lymphoma Diagnosis and Treatment
- Advanced Proteomics Techniques and Applications
- Ubiquitin and proteasome pathways
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Genomics and Chromatin Dynamics
- HER2/EGFR in Cancer Research
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Metabolomics and Mass Spectrometry Studies
- Nuclear Structure and Function
- Peptidase Inhibition and Analysis
- RNA regulation and disease
- Bacterial Genetics and Biotechnology
- Microtubule and mitosis dynamics
- Genomics and Phylogenetic Studies
- Lipid Membrane Structure and Behavior
- Enzyme Structure and Function
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
Universitätsmedizin Göttingen
2016-2025
Max Planck Institute for Multidisciplinary Sciences
2021-2025
University of Göttingen
2016-2025
Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103
2023-2025
Max Planck Institute for Biophysical Chemistry
2015-2024
Universitätsklinikum Würzburg
2024
Max Planck Institute of Biochemistry
2024
Multiscale Bioimaging
2023-2024
Max Planck Institute for the Study of Religious and Ethnic Diversity
2024
Bioanalytica (Switzerland)
2015-2024
Synaptic vesicle recycling has long served as a model for the general mechanisms of cellular trafficking. We used an integrative approach, combining quantitative immunoblotting and mass spectrometry to determine protein numbers; electron microscopy measure organelle numbers, sizes, positions; super-resolution fluorescence localize proteins. Using these data, we generated three-dimensional "average" synapse, displaying 300,000 proteins in atomic detail. The copy numbers involved same step...
Elongation factor P (EF-P) is a translation of unknown function that has been implicated in great variety cellular processes. Here, we show EF-P prevents ribosome from stalling during synthesis proteins containing consecutive prolines, such as PPG, PPP, or longer proline strings, natural and engineered model proteins. promotes peptide-bond formation stabilizes the peptidyl-transfer RNA catalytic center ribosome. posttranslationally modified by hydroxylated β-lysine attached to lysine...
Significance Tau is an important microtubule-associated protein. Although the structure–function relationship of has been intensively studied for many years primarily by molecular biology and biochemical approaches, little still known about mechanisms which interacts with microtubules promotes microtubule assembly. Here, we provide detailed insight into Tau–microtubule association using NMR spectroscopy mass spectrometry. We show that binds to small groups residues, are pathological...
In neurodegenerative diseases such as Alzheimer's disease (AD), Parkinson's (PD) and prion diseases, deposits of aggregated disease-specific proteins are found. Oligomeric aggregates presumed to be the key neurotoxic agent. Here we describe novel oligomer modulator anle138b [3-(1,3-benzodioxol-5-yl)-5-(3-bromophenyl)-1H-pyrazole], an aggregation inhibitor developed based on a systematic high-throughput screening campaign combined with medicinal chemistry optimization. vitro, blocked...
The turnover of brain proteins is critical for organism survival, and its perturbations are linked to pathology. Nevertheless, protein lifetimes have been difficult obtain in vivo. They readily measured vitro by feeding cells with isotopically labeled amino acids, followed mass spectrometry analyses. In vivo generated from at least two sources: acids the diet, non-labeled degradation pre-existing proteins. This renders measurements difficult. Here we solved this problem rigorously a workflow...
C-type lectin receptors (CLRs) that couple with the kinase Syk are major pattern recognition for activation of innate immunity and host defense. CLRs recognize fungi other forms microbial or sterile danger, they induce inflammatory responses through adaptor protein Card9. The mechanisms relaying CLR proximal signals to core Card9 module unknown. Here we demonstrated C-δ (PKCδ) was activated upon Dectin-1-Syk signaling, mediated phosphorylation at Thr231, responsible Card9-Bcl10 complex...