Frank Brombacher
A5048518247- Immune Cell Function and Interaction
- Parasites and Host Interactions
- Research on Leishmaniasis Studies
- IL-33, ST2, and ILC Pathways
- Asthma and respiratory diseases
- T-cell and B-cell Immunology
- Trypanosoma species research and implications
- Tuberculosis Research and Epidemiology
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Mycobacterium research and diagnosis
- Parasite Biology and Host Interactions
- Allergic Rhinitis and Sensitization
- Immunotherapy and Immune Responses
- Helminth infection and control
- Immune cells in cancer
- Toxin Mechanisms and Immunotoxins
- Parasitic Diseases Research and Treatment
- Urticaria and Related Conditions
- Dermatology and Skin Diseases
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related molecular mechanisms research
- Food Allergy and Anaphylaxis Research
- Pediatric health and respiratory diseases
Wellcome Centre for Infectious Diseases Research in Africa
2009-2025
University of Cape Town
2016-2025
International Centre for Genetic Engineering and Biotechnology
2016-2025
South African Medical Research Council
2016-2025
Centro de Ingeniería Genética y Biotecnología
2018-2024
North-West University
2022
International Centre for Genetic Engineering and Biotechnology
2008-2020
University of Aberdeen
2015
City of Cape Town
2011
Institute of Immunology
2011
The pathogenesis of asthma reflects, in part, the activity T cell cytokines. Murine models support participation interleukin-4 (IL-4) and IL-4 receptor asthma. Selective neutralization IL-13, a cytokine related to that also binds α chain receptor, ameliorated phenotype, including airway hyperresponsiveness, eosinophil recruitment, mucus overproduction. Administration either IL-13 or conferred an asthma-like phenotype nonimmunized cell–deficient mice by chain–dependent pathway. This pathway...
Natural antibodies are often dismissed from immunological analysis as “background,” but they may play an important role in conferring immunity against infections. In antibody-free mice infected with various viruses or Listeria monocytogenes , viral bacterial titers peripheral organs, including the kidney and brain, were 10 to 100 times greater than antibody-competent (and enhanced their susceptibility some infections), secondary lymphoid organs lower mice. Thus, natural a crucial by...
Active suppression of tumor-specific T lymphocytes can limit the efficacy immune surveillance and immunotherapy. While tumor-recruited CD11b+ myeloid cells are known mediators tumor-associated dysfunction, true nature these suppressive fine biochemical pathways governing their immunosuppressive activity remain elusive. Here we describe a population circulating CD11b+IL-4 receptor α+ (CD11b+IL-4Rα+), inflammatory-type monocytes that is elicited by growing tumors activated IFN-γ released from...
Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets genes has not been previously studied en masse. Exploiting the fact active are transcribed, we simultaneously measured their activity in 19 human 14 mouse time courses covering a wide range cell types biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated earliest responses....
T helper (Th)17 cells producing interleukin (IL)-17 play a role in autoimmune and allergic inflammation. Here, we show that IL-23 induces IL-17 the lung is required during antigen sensitization to develop asthma, as shown IL-17R–deficient mice. Since expression increased further upon challenge, addressed its function effector phase. Most strikingly, neutralization of augmented response sensitized Conversely, exogenous reduced pulmonary eosinophil recruitment bronchial hyperreactivity,...
Abstract Alternatively activated macrophages (aaMφ) display molecular and biological characteristics that differ from those of classically (caMφ). Recently, we described an experimental model murine trypanosomosis in which the early stage infection mice with a Trypanosoma brucei variant is characterized by development caMφ, whereas late chronic stages infection, aaMφ develop. In present study, used suppression subtractive hybridization (SSH) to identify genes are expressed differentially...
Previous studies demonstrate that aluminium hydroxide adjuvant (alum) produces increased Th1 responses in IL-4-deficient mice compared with wild-type animals, although the continued production of IL-5 by spleen cells from these also indicates Th2 are induced. In present study, we alum can induce Th2-associated IL-4 and absence signaling deficient either IL-4Ralpha or Stat6. The observed could not be due to IL-13 as impaired IL-4Ralpha- Stat6-deficient mice. We detected higher levels...
Interleukin (IL)-25 is a member of the IL-17 family cytokines. However, unlike other members this family, IL-25 promotes T helper (Th) 2 responses. We now show that also regulates development autoimmune inflammation mediated by IL-17-producing cells. have generated IL-25-deficient (il25-/-) mice and found they are highly susceptible to experimental encephalomyelitis (EAE). The accelerated disease in il25-/- associated with an increase IL-23 periphery subsequent number inflammatory IL-17-,...
Macrophages (MΦs) colonize tissues during inflammation in two distinct ways: recruitment of monocyte precursors and proliferation resident cells. We recently revealed a major role for IL-4 the proliferative expansion MΦs Th2-biased tissue nematode infection. now show that intestinal as well infection is restricted to sites production requires MΦ-intrinsic IL-4R signaling. However, both IL-4Rα–dependent –independent mechanisms contributed MΦ infections. IL-4R–independent was controlled by...
Background. Statins are cholesterol-lowering drugs, targeting HMG-CoA reductase, thereby reducing the risk of coronary disorders and hypercholesterolemia. However, they also can influence immunologic responses. Methods. Peripheral blood mononuclear cells (PBMCs) monocyte-derived macrophages (MDMs) were isolated from patients with familial hypercholesterolemia (FH) during statin therapy. After infection Mycobacterium tuberculosis, bacterial burden was determined. In vivo, mice treated statins...
C-type lectin receptors (CLRs) that couple with the kinase Syk are major pattern recognition for activation of innate immunity and host defense. CLRs recognize fungi other forms microbial or sterile danger, they induce inflammatory responses through adaptor protein Card9. The mechanisms relaying CLR proximal signals to core Card9 module unknown. Here we demonstrated C-δ (PKCδ) was activated upon Dectin-1-Syk signaling, mediated phosphorylation at Thr231, responsible Card9-Bcl10 complex...
Abstract In the FANTOM5 project, transcription initiation events across human and mouse genomes were mapped at a single base-pair resolution their frequencies monitored by CAGE (Cap Analysis of Gene Expression) coupled with single-molecule sequencing. Approximately three thousands samples, consisting variety primary cells, tissues, cell lines, time series samples during activation development, subjected to uniform pipeline data production. The analysis started measuring RNA extracts assess...
Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in mammalian genomes, and yet, their functions remain largely unknown. As part FANTOM6 project, we systematically knocked down expression 285 lncRNAs human dermal fibroblasts quantified cellular growth, morphological changes, transcriptomic responses using Capped Analysis Gene Expression (CAGE). Antisense oligonucleotides targeting same exhibited global concordance, molecular phenotype, measured by CAGE, recapitulated...
IL-4 receptor α chain (IL-4Rα)-deficient mice were generated by gene-targeting in BALB/c embryonic stem cells. Mutant showed a loss of signal transduction and functional activity. The lack IL-4Rα resulted markedly diminished, but not absent, TH2 responses after infection with the helminthic parasite Nippostrongylus brasiliensis . CD4 + , CD62L-high, CD62L-low T cell populations from uninfected −/− isolated sorting. Upon primary stimulation cross-linkage, CD62L-low, cells secreted...