A5042551199- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Gut microbiota and health
- IL-33, ST2, and ILC Pathways
- Dermatology and Skin Diseases
- Asthma and respiratory diseases
- Urticaria and Related Conditions
- Escherichia coli research studies
- Helicobacter pylori-related gastroenterology studies
- Clostridium difficile and Clostridium perfringens research
- Cancer Immunotherapy and Biomarkers
- Lymphatic System and Diseases
- Diabetes and associated disorders
- Probiotics and Fermented Foods
- CAR-T cell therapy research
- Microscopic Colitis
- Transgenic Plants and Applications
- Food Allergy and Anaphylaxis Research
- Immune cells in cancer
- Pancreatic function and diabetes
- Research on Leishmaniasis Studies
- Cytomegalovirus and herpesvirus research
- Bacterial Infections and Vaccines
- Respiratory and Cough-Related Research
Washington University in St. Louis
2015-2025
Center for Rheumatology
2008-2010
Howard Hughes Medical Institute
1993-2010
University of Washington
2002-2006
Murphy Oil Corporation (United States)
1996
University of Chicago
1991
Development of the appropriate CD4 + T helper (T H ) subset during an immune response is important for disease resolution. With use naive, ovalbumin-specific αβ cell receptor transgenic cells, it was found that heat-killed Listeria monocytogenes induced 1 development in vitro through macrophage production interleukin-12 (IL-12). Moreover, inhibition IL-12 may explain ability IL-10 to suppress development. Murine responses L. vivo are phenotype. Therefore, this regulatory pathway have evolved...
Abstract Dendritic cells are APCs that unique in their potency to stimulate proliferation of primary Ag-specific responses vitro and vivo. In this study, we demonstrate dendritic can produce IL-12, a dominant cytokine involved the development IFN-gamma-producing T cells. This finding resulted from our observations cell-induced Th1 total CD4+ upon neutralization endogenous levels IL-4 was IL-12-dependent. Furthermore, induce naive LECAM-1bright obtained alpha beta-TCR transgenic mice,...
To address the mechanisms controlling T helper (Th) phenotype development, we used DO10, a transgenic mouse line that expresses alpha beta T-cell receptor from an ovalbumin-reactive hybridoma, as source of naive cells can be stimulated in vitro with ovalbumin peptide presented by defined antigen-presenting (APCs). We have examined role cytokines and APCs regulation Th development. Interleukin 4 (IL-4) directs development toward Th2 phenotype, stimulating IL-4 silencing IL-2 interferon gamma...
Tolerogenic T cells need probiotics CD4 + CD8αα double-positive intraepithelial lymphocytes (DP IELs) are a recently discovered class of intestinal believed to take part in variety immune responses, including oral tolerance. These absent germ-free mice, but the mechanisms driving their development unclear. Cervantes-Barragan et al. found that particular species probiotic bacteria, Lactobacillus reuteri , induces DP IELs. This does not occur by stimulating system directly. Instead, L....
A host's ability to resist certain pathogens such as Leishmania major can depend upon the phenotype of T helper (Th) subset that develops. Different murine genetic backgrounds are known significantly alter direction Th development, although cellular basis this influence is poorly understood. To examine effect we used an in vitro alpha/beta-T cell receptor (TCR) transgenic system for analysis development. control TCR usage, derived DO11.10 alpha/beta-TCR transgene several backgrounds. Our...
Tumors evade immune destruction by actively inducing tolerance through the recruitment of CD4(+)CD25(+)Foxp3(+) regulatory T cells (Treg). We have previously described increased prevalence these in pancreatic adenocarcinoma, but it remains unclear what mechanisms are involved recruiting Tregs into tumor microenvironment. Here, we postulated that chemokines might direct Treg homing to tumor. show, both human adenocarcinoma and a murine model (Pan02), produce levels ligands for CCR5 chemokine...
We have previously shown that dendritic cells isolated after overnight culture, which can express B7 and are potent stimulators of naive T cell proliferation, relatively poor at inducing the proliferation a panel murine helper 1 (Th1) clones. Maximal stimulation Th1 clones was achieved using unseparated splenic antigen presenting (APC). An explanation for these findings is provided in present study where we show FcR+ L transfected with stimulate minimal response to anti-CD3 antibodies,...
Gut bacterial strains targeted by IgA in undernourished Malawian children produce severe enteropathy gnotobiotic mice and correlate with health status.
Bacterial antigen encounter in a preweaning interval is critical for developing antigen-specific tolerance to gut bacteria.
The genetic background of T lymphocytes influences development the helper (T H ) phenotype, resulting in either resistance or susceptibility certain mouse strains to pathogens such as Leishmania major . With an vitro model system, a difference maintenance responsiveness cells interleukin-12 (IL-12) was detected between BALB/c and B10.D2 mice. Although naïve from both initially responded IL-12, lost IL-12 after stimulation with antigen vitro, even when cocultured cells. Thus, mice infection...
Although regulatory T (T reg) cells are thought to develop primarily in the thymus, peripheral events that shape protective reg cell population unclear. We analyzed CD4(+) receptor (TCR) repertoire by cellular phenotype and location mice with a fixed TCRbeta chain. found (Foxp3(+)) showed marked skewing of TCR usage anatomical manner similar antigen-experienced (CD44(hi)Foxp3(-)) but not naive (CD44(lo)Foxp3(-)) cells, even though CD44(hi) used mostly dissimilar TCRs. This was likely...
Commensal bacteria shape the colonic regulatory T (Treg) cell population required for intestinal tolerance. However, little is known about this process. Here, we use transfer of naive commensal-reactive transgenic cells expressing Treg receptors (TCRs) to study peripheral (pTreg) development in normal hosts. We found that were activated primarily distal mesenteric lymph node. induction was rapid, generating >40% Foxp3(+) 1 week after transfer. Contrary prior reports, underwent most...