A5031296657- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Inflammation biomarkers and pathways
- Neuroinflammation and Neurodegeneration Mechanisms
- Eosinophilic Esophagitis
- Immune cells in cancer
- CAR-T cell therapy research
- Cancer Research and Treatments
- interferon and immune responses
- Cancer Immunotherapy and Biomarkers
- Whipple's Disease and Interleukins
- HIV Research and Treatment
- Inflammatory Bowel Disease
- Immunodeficiency and Autoimmune Disorders
- Virus-based gene therapy research
- Reproductive System and Pregnancy
- Medicinal Plants and Neuroprotection
- Autoimmune and Inflammatory Disorders Research
- Autoimmune and Inflammatory Disorders
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Microscopic Colitis
Washington University in St. Louis
2016-2025
Matsumoto Dental University
2019
Hope Center for Neurological Disorders
2019
University of Toronto
2019
Case Western Reserve University
2019
National Institutes of Health
2019
Pulmonary and Critical Care Associates
2015
Pediatrics and Genetics
2015
Institute of Molecular Biology and Biophysics
2015
University of Missouri–St. Louis
2011
We have previously demonstrated that human peripheral blood low density mononuclear cells cultured in granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 develop into dendritic (DCs) are extremely efficient presenting soluble antigens to T cells. To identify the mechanisms responsible for antigen capture, we studied endocytic capacity of DCs using fluorescein isothiocyanate-dextran, horseradish peroxidase, lucifer yellow. found use two distinct capture. The first...
We investigated the possibility that T helper cells might enhance stimulatory function of dendritic (DCs). found ligation CD40 by CD40L triggers production extremely high levels bioactive IL-12. Other stimuli such as microbial agents, TNF-alpha or LPS are much less effective not at all. In addition, is most potent stimulus in upregulating expression ICAM-1, CD80, and CD86 molecules on DCs. These effects result an increased capacity DCs to trigger proliferative responses IFN-gamma cells....
The innate immune system recognizes viral dsRNA through two distinct pathways; the Toll-like receptor 3 (TLR3) pathway detects phagocytosed in endosomes; helicases retinoic acid-induced protein I (RIG-I) and melanoma differentiation-associated gene-5 (mda-5) detect cytoplasmic generated during replication. Both RIG-I mda-5 can bind polyriboinosinic:polyribocytidylic acid (polyI:C), synthetic analog of dsRNA, mediate type IFN responses to polyI:C multiple RNA viruses vitro. We mda-5-deficient...
Natural killer (NK) cell–mediated lysis is negatively regulated by cell inhibitory receptors specific for major histocompatibility complex (MHC) class I molecules. In this study, we characterize a novel MHC receptor of the immunoglobulin-superfamily, expressed not only subsets NK and T cells, but also B monocytes, macrophages, dendritic cells. This receptor, called Ig-like transcript (ILT)2, binds molecules delivers negative signal that inhibits killing as well Ca2+ mobilization in cells...
Plasmacytoid dendritic cells are present in lymphoid and nonlymphoid tissue contribute substantially to both innate adaptive immunity. Recently, we have described several monoclonal antibodies that recognize a plasmacytoid cell-specific antigen, which termed BDCA-2. Molecular cloning of BDCA-2 revealed is novel type II C-type lectin, shows 50.7% sequence identity at the amino acid level its putative murine ortholog, cell-associated lectin 2. Anti-BDCA-2 rapidly internalized efficiently...
The initiation of an immune response is critically dependent on the activation dendritic cells (DCs). This process triggered by surface receptors specific for inflammatory cytokines or conserved patterns characteristic infectious agents. Here we show that human DCs are activated influenza virus infection and double-stranded (ds)RNA. results not only in increased antigen presentation T cell stimulatory capacity, but also resistance to cytopathic effect virus, mediated production type I...
Tolerogenic T cells need probiotics CD4 + CD8αα double-positive intraepithelial lymphocytes (DP IELs) are a recently discovered class of intestinal believed to take part in variety immune responses, including oral tolerance. These absent germ-free mice, but the mechanisms driving their development unclear. Cervantes-Barragan et al. found that particular species probiotic bacteria, Lactobacillus reuteri , induces DP IELs. This does not occur by stimulating system directly. Instead, L....
Triggering receptor expressed on myeloid cells 2 (TREM2) is a microglial that recognizes changes in the lipid microenvironment, which may occur during amyloid β (Aβ) accumulation and neuronal degeneration Alzheimer's disease (AD). Rare TREM2 variants affect function lead to an increased risk of developing AD. In murine models AD, deficiency prevents clustering around Aβ deposits. However, origin surrounding impact are matter debate. Using parabiosis, we found amyloid-associated derive from...
Abstract The triggering receptor expressed on myeloid cells 2 (TREM-2) delivers intracellular signals through the adaptor DAP12 to regulate cell function both within and outside immune system. role of TREM-2 in immunity has been obscured by failure detect expression protein vivo. In this study, we show that is macrophages infiltrating tissues from circulation alternative activation with IL-4 can induce TREM-2. abrogated macrophage maturation LPS IFN-γ. Using TREM-2−/− mice, find functions...
Abstract In humans, the type I interferon (IFN) family consists of 13 IFN‐α subtypes, IFN‐β and IFN‐o newly discovered IFN‐like IFN‐λ1, ‐λ2 ‐λ3. We have investigated expression λ IFN genes following virus infections or Toll‐like receptor (TLR) triggering in monocyte‐derived DC (MDDC) plasmacytoid (pDC). found thatall IFN‐α, ‐β, ‐o ‐λ subtypes are expressed influenza‐virus‐infected MDDC pDC. Conversely, differential gene transcription was induced pDC stimulated by specific TLR agonists. TLR‐9...