A5020928967- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cytokine Signaling Pathways and Interactions
- Liver Diseases and Immunity
- Liver Disease Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Blood transfusion and management
- Immune Response and Inflammation
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Liver physiology and pathology
- Trypanosoma species research and implications
- Immune cells in cancer
- TGF-β signaling in diseases
- Research on Leishmaniasis Studies
- interferon and immune responses
- Hemoglobinopathies and Related Disorders
- Blood donation and transfusion practices
- Erythrocyte Function and Pathophysiology
- Cancer Cells and Metastasis
- NF-κB Signaling Pathways
- Cellular Mechanics and Interactions
- Skin and Cellular Biology Research
- COVID-19 Impact on Reproduction
- Viral gastroenteritis research and epidemiology
- IL-33, ST2, and ILC Pathways
University of Virginia
2015-2024
University of Virginia Health System
2019-2024
University of North Georgia
2019
Dartmouth–Hitchcock Medical Center
2008-2015
Dartmouth College
2003-2015
Great Western Hospital
2015
Park Plaza Hospital
2012
Level (Czechia)
2012
Boston University
2008
Hemanext (United States)
2008
This guideline has been approved by the American Association for Study of Liver Diseases (AASLD) and represents position Association. Clinical practice guidelines are defined as "systematically developed statements to assist practitioner patient decisions about appropriate heath care specific clinical circumstances."1 (All references available in Supporting Information.) These on autoimmune hepatitis provide a data-supported approach diagnosis management this disease. They based following:...
In this study, the joining sequences between chromosomes 14 and 18 on 14q + of a patient with pre-B-cell leukemia four patients follicular lymphoma carrying t(14;18) chromosome translocation were analyzed. each case, involved segment has recombined immunoglobulin heavy-chain (J H ) 14. The sites recombination are located close to 5′ end J segment, where diversity (D) regions rearranged segments in production active genes. As extraneous nucleotides (N regions) observed at specific signal-like...
Bone marrow stromal cells [BMSCs; also known as mesenchymal stem (MSCs)] effectively suppress inflammatory responses in acute graft-versus-host disease humans and a number of models mice. Many the studies concluded that BMSC-driven immunomodulation is mediated by suppression proinflammatory Th1 while rebalancing Th1/Th2 ratio toward Th2. In this study, using ragweed induced mouse asthma model, we studied if BMSCs could be beneficial an allergic, Th2-dominant environment. When were injected...
The genetic background of T lymphocytes influences development the helper (T H ) phenotype, resulting in either resistance or susceptibility certain mouse strains to pathogens such as Leishmania major . With an vitro model system, a difference maintenance responsiveness cells interleukin-12 (IL-12) was detected between BALB/c and B10.D2 mice. Although naïve from both initially responded IL-12, lost IL-12 after stimulation with antigen vitro, even when cocultured cells. Thus, mice infection...
Interleukin 12 (IL-12) strongly augments gamma interferon production by natural killer (NK) and T cells. IL-12 also promotes effective cell-mediated immune responses, which are particularly important against intracellular bacteria such as Listeria monocytogenes. While the lipopolysaccharide (LPS) of gram-negative induces monocyte IL-12, relevant gram-positive components induce uncharacterized. We used human monocytic cell line THP-1 to study induction bacteria. Muramyl dipeptides well major...
Differential screening of a cDNA library from the PC12 rat pheochromocytoma cell line previously revealed clone, clone 73, whose corresponding mRNA is induced by nerve growth factor (NGF). Induction parallels NGF-stimulated differentiation chromaffinlike phenotype to sympathetic neuronlike phenotype. We report that DNA sequence analysis reveals 73 encodes an intermediate filament (IF) protein predicted amino acid distinct known sequences other members IF family. The has highest homology with...
Genetic background of the T cell can influence helper (Th) phenotype development, with some murine strains (e.g., B10.D2) favoring Th1 development and others BALB/c) Th2 development. Recently we found that B10.D2 exhibit an intrinsically greater capacity to maintain interleukin 12 (IL-12) responsiveness under neutral conditions in vitro compared BALB/c cells, allowing for prolonged undergo IL-12-induced To begin identification loci controlling this genetic effect, used a T-cell antigen...
Abstract Red blood cells (RBCs) are the most numerous cell type in body and serve a vital purpose of delivering oxygen to essentially all tissues. In addition central role RBCs health disease, RBC storage is requirement for >90 million units transfusions given millions recipients each year, worldwide. It well known that there genetic donor-to-donor variability how human store, rendering nonstandardized therapeutic with wide range biological properties from unit unit, by time it...
Abstract TGF-β1 plays a critical role in restraining pathogenic Th1 autoimmune responses vivo, but the mechanisms that mediate TGF-β1’s suppressive effects on CD4+ T cell expression of IFN-γ remain incompletely understood. To evaluate by which inhibits cells, we primed naive wild-type murine BALB/c cells vitro under development conditions presence or absence added TGF-β1. We found during priming suppressed both as well effector expressing at recall stimulation. inhibited IFN-γ-expressing...
Immune-mediated liver injury in hepatitis is due to activated T cells producing interferon-γ (IFN-γ). It important identify negative feedback immune mechanisms that can regulate cell activity. In this study, we demonstrate inflammation mediated by type 1 helper (Th1) induce the accumulation of myeloid-derived suppressor (MDSCs), pleiomorphic capable modulating cell–mediated immunity, heretofore have been studied almost exclusively context tumor-associated inflammation. Mice deficient gene...
Significance Understanding dendritic cell (DC) migration during an immune response is fundamental to defining the rules that govern T cell-mediated immunity. We recently described mice deficient in pattern recognition receptor NLRP10 (NLR family, pyrin domain containing 10) with a severe DC defect. Using whole-exome sequencing, we discovered this defect was due mutation of guanine nucleotide exchange factor Dock8 (dedicator cytokinesis 8). DOCK8 regulates cytoskeleton dynamics leukocytes,...
Parasitoids are among the most important natural enemies of insects in many environments. Acyrthosiphon pisum, pea aphid, is a common pest leguminous crops temperate regions. Pea aphids frequently attacked by range endoparasitic wasps, including aphidiine, Aphidius ervi. Immunity to parasitoid attack thought involve secondary symbiotic bacteria, presence which associated with death egg. It has been suggested that there fecundity cost resistance, as individuals carrying symbionts resistance...
Interleukin 12 (IL-12)-induced T helper 1 (Th1) development requires Stat4 activation. However, antigen-activated Th1 cells can produce interferon γ (IFN-γ) independently of IL-12 and Thus, in differentiated cells, factors regulated by may be involved IFN-γ production. Using subtractive cloning, we identified ERM, an Ets transcription factor, to a Th1-specific, IL-12-induced gene. IL-12-induction ERM occurred wild-type Stat1-deficient, but not Stat4-deficient, suggesting is Stat4-inducible....
Abstract Expression of IL-12Rs is one important checkpoint for Th1 development. BALB/c DO11.10 CD4+ T cells stimulated by Ag in neutral conditions lose expression the IL-12R β2 subunit and become unresponsive to IL-12. In contrast, B10.D2 or F1 (BALB/c × B10.D2) maintain IL-12Rβ2 when similarly. Here we show that loss IL-12 responsiveness involves action endogenous TGF-β. presence anti-TGF-β specifically responsiveness, express mRNA, can stimulate nitric oxide production peritoneal exudate...
Abstract Autoimmune hepatitis (AIH) in humans arises spontaneously genetically susceptible individuals and is associated with the presence of Th1 cells liver. The understanding AIH has advanced more slowly than that other organ-specific autoimmune diseases, however, largely because lack an appropriate animal model. We now describe a new mouse model characterized by spontaneous development necroinflammatory restricted genetic background. Mice deficient immunomodulatory cytokine TGF-β1 were...
Abstract TGF-β1 prevents the development of autoimmune disease by restraining autoreactive Th1 cells. inhibits in part suppressing expression T-bet, an IFN-γ-induced transcription factor that promotes differentiation, but how suppresses T-bet is not known. In this study we show through hemopoietic protein tyrosine phosphatase (PTP) Src homology region 2 domain-containing phosphatase-1 (Shp-1). murine CD4+ T cells, IFN-γ rapidly induced as well IFN regulatory factor-1, another important for...
Red cell (RBC) storage can be extended to 9 weeks under anaerobic or alkaline conditions. Simultaneous use of these approaches has not provided additive benefit. Our objective was determine whether with acidified solution (AS) coupled metabolic rejuvenation might further improve the benefits storage.RBC in AS a pH value 6.5, 7.4, 8.3 aerobic conditions examined using panel vitro biochemical and RBC markers. during cold also evaluated. A randomized crossover radiolabeled recovery study (eight...