A5004253231- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Immune Cell Function and Interaction
- Offshore Engineering and Technologies
- Drilling and Well Engineering
- T-cell and B-cell Immunology
- Neuroendocrine regulation and behavior
- Virus-based gene therapy research
- Social and Educational Sciences
- Historical and Archaeological Studies
- Animal testing and alternatives
- Molecular Biology Techniques and Applications
- Liver Disease Diagnosis and Treatment
- Genetic Mapping and Diversity in Plants and Animals
- Helicobacter pylori-related gastroenterology studies
- Stress Responses and Cortisol
- Oil and Gas Production Techniques
- Animal Genetics and Reproduction
- Public Spaces through Art
- Veterinary medicine and infectious diseases
- Veterinary Pharmacology and Anesthesia
- Engineering and Environmental Studies
- Mycobacterium research and diagnosis
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
University of Copenhagen
2022
Radboud University Nijmegen
2021
Halliburton (United Kingdom)
2019
University of Algarve
2015
Taconic (United States)
2008-2014
We recently investigated the applicability of antibiotic-treated recipient mice for transfer different gut microbiota profiles. With this addendum we elaborate on perspectives and limitations using antibiotics as an alternative to germ-free (GF) technology in microbial transplantation studies, speculate housing effect. It is possible host phenotypes via fecal animals, but problems with reproducibility, baseline values, antibiotic resistance genes should be considered. GF animals maintained...
Transplantation of germ-free (GF) mice with microbiota from or humans stimulates the intestinal immune system in disparate ways. We transplanted a human into GF C57BL/6 and murine Swiss-Webster (SW) mice. Mice were bred to produce an offspring generation. 56% Operational Taxonomic Units (OTUs) present donor established recipient mice, whereas 81% OTUs SW Anti-inflammatory bacteria such as Faecalibacterium Bifidobacterium not transferred Expression immune-related genes was lower...
Germ-free rodents colonized with microbiotas of interest are used for host-microbiota investigations and testing microbiota-targeted therapeutic candidates. Traditionally, isolators housing such gnotobiotic due to optimal protection from the environment, but research groups focused on microbiome increasingly combining or substituting isolator individually ventilated cage (IVC) systems. We compared effect systems gut microbiota composition germ-free mice a complex housed in either multiple...
Rats with implanted telemetry transponders were blood sampled by jugular puncture, periorbital puncture or tail vein in carbon dioxide (CO 2 ), isoflurane without anaesthesia a crossover design. Heart rate, pressure and body temperature registered for three days after sampling. Initially increased, but shortly sampling it decreased, which led to increased heart rate. Sampling induced rapid fluctuations temperature, an increase temperature. Generally, rats recovered from within 2–3 h, except...
Abstract Laboratory mice live in specific pathogen‐free (SPF) conditions, resulting an immature immune system comparable to that of newborns rather than adult humans or from pet shops. This condition may compromise their translational value. Reintroducing pathogens would lead the uncontrolled spread infections and associated diseases, so research facilities should seek safer alternatives. We immunized laboratory with a cocktail pathogens, which were inactivated by ultraviolet irradiation...
Pre-immunization with inactivated antigens has been developed as an alternative to the use of 'dirty' mice, which in contrast specific pathogen free (SPF) harbour a range pathogens. Within certain research areas, such mice are considered better models for humans than SPF they have immune system that mirrors human immunity. We murine adenovirus type 1 (FL), minute virus mouse hepatitis (A59), respirovirus muris (Sendai), Theiler's encephalomyelitis (GD7) and
Abstract The operator set challenging rate of penetration (ROP) targets on a six-well campaign in the Norwegian Sea, with total depths between 6000 and 7000 m (19,500 to 22,750 ft) initial 70 m/h (17.5-in. interval) 120 (12.25-in. interval). To support these ROPs, cuttings slops management system should provide higher conveyance storage capacities than typically observed during drilling operations. When specified ROPs were achieved, targeted rates increased subsequent wells. Collaboration...
Abstract Laboratory mice show poor translation to humans within a range of pre-clinical fields, which might be due their specific pathogen free (SPF) status. SPF have CD8 + effector memory T cell levels more comparable newborn than adult or pet shop mice. However, reintroducing pathogens would re-introduce diseases, and, therefore, research facilities should look for safer alternatives. We inactivated mouse adenovirus type 1, minute virus mice, hepatitis virus, Sendai Theiler’s...
ICLAS Laboratory Animal Quality Network (LAQN) programs currently consist of the Performance Evaluation Program (PEP), which focuses on microbial monitoring by and for laboratory animal diagnostic laboratories, Genetic Reference Monitoring (GENRef), provides assay-ready reference DNA genetic testing mouse strains. Since 2008, PEP has grown to become a truly international program with participating laboratories in 5 continents. Launched 2016, GENRef distributes from 12 common inbred strains...