A5040106526- T-cell and B-cell Immunology
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Dermatology and Skin Diseases
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Acne and Rosacea Treatments and Effects
- Retinoids in leukemia and cellular processes
- Skin Protection and Aging
- Urticaria and Related Conditions
- Allergic Rhinitis and Sensitization
- Antimicrobial Peptides and Activities
- Pharmacological Effects of Natural Compounds
- Inflammasome and immune disorders
- Research on Leishmaniasis Studies
- Adrenal Hormones and Disorders
- Kawasaki Disease and Coronary Complications
- Pluripotent Stem Cells Research
- Macrophage Migration Inhibitory Factor
- Phagocytosis and Immune Regulation
- Systemic Lupus Erythematosus Research
- Cytokine Signaling Pathways and Interactions
- Reproductive System and Pregnancy
- PI3K/AKT/mTOR signaling in cancer
Galderma (United States)
2023
Galderma (Switzerland)
2021-2023
AstraZeneca (Sweden)
2016-2022
Galderma (France)
2021
Yale University
2013-2017
Medizinische Hochschule Hannover
2010-2012
HTG Molecular Diagnostics (United States)
2010
Philipps University of Marburg
2010
Center for Rheumatology
2010
Conventional DC2s are necessary and sufficient for priming T follicular helper cells.
Evidence suggests that distinct splenic dendritic cell (DC) subsets activate either CD4+ or CD8+ T cells in vivo. This bias has been partially ascribed to differential antigen presentation; however, all DC can both lineages vitro. Therefore, we tested whether the organization of and spleen dictated this preference. We discovered segregated within zones prior immunization. After intravenous immunization, two major conventional populations, distinguished by 33D1 XCR1 staining, migrated into...
Red blood cell (RBC) transfusion is a life-saving therapeutic tool. However, major complication in recipients the generation of antibodies against non-ABO alloantigens on donor RBCs, potentially resulting hemolysis and renal failure. Long-lived antibody responses typically require CD4(+) T help and, murine models, alloimmunization requires spleen. Yet, it not known how RBC-derived antigens are presented to naive cells We sought answer whether splenic dendritic (DCs) were essential for...
BackgroundPrurigo nodularis (PN) is a debilitating, difficult-to-treat, intensely pruritic, chronic inflammatory skin disease characterized by hyperkeratotic nodules. The pathogenesis of PN not well understood but believed to involve cross talk between sensory nerve fibers, immune cells, and the epidermis. It centered around neuroimmune cytokine IL-31, driving an intractable itch–scratch cycle.ObjectiveWe sought provide comprehensive view transcriptomic changes in characterize mechanism...
Significance Understanding dendritic cell (DC) migration during an immune response is fundamental to defining the rules that govern T cell-mediated immunity. We recently described mice deficient in pattern recognition receptor NLRP10 (NLR family, pyrin domain containing 10) with a severe DC defect. Using whole-exome sequencing, we discovered this defect was due mutation of guanine nucleotide exchange factor Dock8 (dedicator cytokinesis 8). DOCK8 regulates cytoskeleton dynamics leukocytes,...
Prurigo nodularis (PN) is a chronic neuroimmune skin disease characterized by bilaterally distributed pruritic hyperkeratotic nodules on extremities and trunk. Neuroimmune dysregulation scratching are believed to both induce maintain the characteristic lesions.
Induced pluripotent stem cells (iPSC) offer the possibility to generate diverse disease-relevant cell types, from any genetic background with use of cellular reprogramming and directed differentiation. This provides a powerful platform for disease modeling, drug screening therapeutics. The critical question is how differentiated iPSC-derived translate their primary counterparts. Our refinement published differentiation protocol produces CD14+ monocytic lineage at higher yield, in smaller...
Prurigo nodularis (PN) is a debilitating skin disease characterized by intense pruritus and hyperkeratotic nodules. Nemolizumab, monoclonal antibody targeting interleukin 31 receptor α, promising novel therapy for the treatment of moderate to severe PN. The biological mechanisms which nemolizumab promotes improvement itch lesions in PN are unknown.To characterize changes plasma protein biomarkers associated with clinical response patients PN.This multicenter cohort study included recruited...
Recent studies highlight the role of Treg in preventing unnecessary responses to allergens and maintaining functional immune tolerance lung. We investigated during sensitization phase a murine model experimental allergic airway inflammation by selectively depleting population vivo. DEpletion REGulatory T cells (DEREG) mice were depleted diphtheria toxin injection. Allergic was induced using OVA as allergen. Pathology assessed scoring for differential cellular infiltration bronchoalveolar...
The property of DC to generate effective CTL responses is influenced by TLR signaling. ligands contain molecular signatures associated with pathogens, have an impact on the antigen processing and presentation DC, are being exploited as potential adjuvants. We hypothesized that TLR2/6 heterodimer agonist S-[2,3-bispalmitoyiloxy-(2R)-propyl]-R-cysteinyl-amido-monomethoxyl polyethylene glycol (BPP), a synthetic derivative Mycoplasma macrophage activating lipopeptide-2, potent adjuvant for...
Interferon γ (IFN-γ) and interleukin 17A (IL-17A)-producing cells are described to be related the protection against Leishmania infantum infection. How immune system coordinates balance between T-helper type 1 (Th1) 17 (Th17) responses during visceral leishmaniasis (VL) is still unknown. Here, we combined transcriptional profiling, using RNA sequencing analysis of human samples, with an experimental model show that Th17-related genes suppressed Th1 signature induced VL. The high amount in VL...
Regulatory T cells (Tregs) play a non-redundant role in maintenance of immune homeostasis. This is achieved by suppressing both, priming naïve and effector cell functions. Although Tregs have been implicated modulating allergic responses, their influence on distinct phases development allergies remains unclear. In this study, using bacterial artificial chromosome (BAC)-transgenic Foxp3-DTR (DEREG) mice we demonstrate that the absence Foxp3+ during allergen challenge surprisingly does not...
During asthma, lung DC capture and process antigens to initiate maintain allergic Th2 cell responses inhaled allergens. The aim of the study was investigate whether allergen-specific IgG, generated during sensitization, can potentiate acute airway inflammation through Fcgamma receptor (FcgammaR)-mediated antigen uptake enhance presentation resulting in augmented T-cell proliferation. We examined impact stimulation on hyperresponsiveness using transgenic gene-deficient mice. Both eosinophilia...
Toll-like receptor (TLR) agonists beneficially modulate allergic airway inflammation. However, the efficiency of TLR varies considerably, and their exact cellular mechanisms (especially 2/6 agonists) are incompletely understood. We investigated at a level whether administration pharmacologically improved TLR2/6 agonist S-[2,3-bispalmitoyiloxy-(2R)-propyl]-R-cysteinyl-amido-monomethoxy polyethylene glycol (BPP) conjugated to antigenic peptide (BPP-OVA) could divert an existing Th2 response...
Background and Objectives: Trifarotene is a topical retinoid selective for retinoic acid receptor gamma that was recently approved treatment of acne vulgaris. We performed gene expression analysis to identify the molecular cellular impact trifarotene on papules. Methods: In this open-label prospective study, subjects with moderate inflammatory back were treated 0.005% or vehicle cream dedicated areas 27 days, 4 biopsies collected from each subject (1 skin without visible lesion three at site...
Tissue transcriptomics is used to uncover molecular dysregulations underlying diseases. However, the majority of studies focus on single diseases with limited relevance for understanding relationship between or identifying disease-specific markers. In this study, we a normalization approach compare gene expression across nine inflammatory skin The normalized datasets were found retain differential signals that allowed unsupervised disease clustering and identification signatures. Using...
Dendritic cells (DCs) are essential for the generation and modulation of cell-mediated adaptive immunity against infections. DC-based vaccination involves transplantation ex vivo-generated DCs loaded with antigen in vitro, but remains limited by number autologous or allogeneic cells. While vitro expansion differentiation hematopoietic stem (HSCs) into seems to be most viable alternative overcome this problem, complexity HSC has posed significant limitations clinical application. We...
In this article, the affiliation for Prof. Holger Garn and Harald Renz was provided incorrectly. The correct is: Department of Clinical Chemistry Molecular Diagnostics, Medical Faculty, Philipps University Marburg, Germany.