A5037574752- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Malaria Research and Control
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Diabetes and associated disorders
- CAR-T cell therapy research
- Herpesvirus Infections and Treatments
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- Dermatology and Skin Diseases
- Complement system in diseases
- Influenza Virus Research Studies
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Viral gastroenteritis research and epidemiology
- Pancreatic function and diabetes
- Hepatitis B Virus Studies
- Invertebrate Immune Response Mechanisms
- Immune cells in cancer
- Adrenal Hormones and Disorders
- SARS-CoV-2 and COVID-19 Research
- Parasites and Host Interactions
The University of Melbourne
2016-2025
Peter Doherty Institute
2016-2025
ARC Centre of Excellence in Advanced Molecular Imaging
2014-2023
Australian Research Council
2015-2022
Northeastern University
2020
Boston University
2020
J. Iverson Riddle Developmental Center
2019
Walter and Eliza Hall Institute of Medical Research
2003-2012
German Rheumatism Research Centre
2010
Bipar
2009
We describe the generation of ovalbumin (OVA)‐specific, MHC class II‐restricted αβ T cell receptor (TCR) transgenic mice. Initial attempts at generating these mice utilized heterologous regulatory elements to drive expression cDNA genes encoding separate α‐ and β‐chains TCR. Unexpectedly, cells bearing TCR failed emerge from thymus in mice, although transgenes did modify endogenous expression. However, subsequent modification approach which enabled β‐chain under control its natural generated...
Class I–restricted presentation is usually associated with cytoplasmic degradation of cellular proteins and often considered inaccessible to exogenous antigens. Nonetheless, certain elements can gain entry into this so-called endogenous pathway by a mechanism termed cross-presentation. This known be effective for class cytotoxic T lymphocyte (CTL) cross-priming directed against variety tumor, viral, minor transplantation The related effect cross-tolerance also effectively eliminate responses...
In this report, we show that cross-presentation of self-antigens can lead to the peripheral deletion autoreactive CD8+ T cells. We had previously shown transfer ovalbumin (OVA)-specific cells (OT-I cells) into rat insulin promoter–membrane-bound form OVA transgenic mice, which express model autoantigen in proximal tubular kidneys, β pancreas, thymus, and testis male led activation OT-I draining lymph nodes. This was due class I–restricted exogenous on a bone marrow–derived antigen presenting...
Ovalbumin (OVA)-specific CD8+ T cells from the cell receptor-transgenic line OT-I (OT-I cells) were injected into unirradiated transgenic RIP-mOVA mice, which express a membrane-bound form of OVA (mOVA) in pancreatic islet beta and renal proximal tubular cells. accumulated draining lymph nodes (LN) kidneys pancreas no other LN. They displayed an activated phenotype proportion entered cycle. Unilateral nephrectomy 7-13 d before inoculation mice allowed to home only regional LN remaining...
Abstract Mouse spleen contains three distinct mature dendritic cell (DC) populations (CD4+8−, CD4−8−, and CD4−8+) which retain a capacity to take up particulate soluble Ags. Although the splenic DC subtypes showed similar uptake of injected OVA, they differed markedly in their present this Ag activate proliferation OVA-specific CD4 or CD8 T cells. For class II MHC-restricted presentation cells, CD8− were more efficient, but for I CD8+ subtype was far effective. This differential persisted...
The classical paradigm for dendritic cell function derives from the study of Langerhans cells, which predominate within skin epidermis. After an encounter with foreign agents, cells are thought to migrate draining lymph nodes, where they initiate T priming. Contrary this, we show here that infection murine epidermis by herpes simplex virus did not result in priming virus-specific cytotoxic lymphocytes cells. Rather, response required a distinct CD8alpha+ subset. Thus, traditional view...
Although circulating memory T cells provide enhanced protection against pathogen challenge, they often fail to do so if infection is localized peripheral or extralymphoid compartments. In those cases, it already resident at the site of virus challenge that offer superior immune protection. These tissue-resident (T RM ) are identified by their expression α-chain from integrin α E (CD103)β 7 , and can exist in disequilibrium with blood, remaining local environment long after infections...
We previously described a mechanism for the maintenance of peripheral self-tolerance. This involves cross-presentation tissue-associated antigens by bone marrow–derived cell type that stimulates proliferation and ultimate deletion self-reactive CD8 T cells. process has been referred to as cross-tolerance. Here, we characterize elusive responsible inducing cross-tolerance CD8α+ dendritic (DC). To achieve this aim, transgenic mice were generated expressing yellow fluorescent protein (YFP)...
Abstract Cross-presentation of cell-bound Ags from established, solid tumors to CD8 cells is efficient and likely have a role in determining host response tumor. A number investigators predicted that when tumor are derived apoptotic either no response, due Ag “sequestration,” or cross-tolerance would ensue. Because the crucial issue whether this happens vivo has never been addressed, we induced apoptosis established hemagglutinin (HA)-transfected AB1 BALB/c mice using apoptosis-inducing...
Secondary lymphoid organs are dominant sites of T cell activation, although many cells subsequently retained within peripheral tissues. Currently, these nonlymphoid compartments viewed as only effector function, without the involvement renewed induction immunity via interactions with professional antigen-presenting cells. We describe a method reactivation herpes simplex virus to examine stimulation tissue-resident during secondary challenge. The results revealed that memory CD8 + responses...
Little is known about the events triggering lymphocyte invasion of pancreatic islets in prelude to autoimmune diabetes. For example, where islet-reactive T cells first encounter antigen has not been identified. We addressed this issue using BDC2.5 cell receptor transgenic mice, which express a recognizing natural islet beta antigen. In animals, activated were found only and lymph nodes draining them, there was close temporal correlation between node activation infiltration. When naive...