A5064246014- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Microscopic Colitis
- Kawasaki Disease and Coronary Complications
- Phagocytosis and Immune Regulation
- CAR-T cell therapy research
- Inflammation biomarkers and pathways
- Pancreatitis Pathology and Treatment
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Colorectal Cancer Treatments and Studies
- Probiotics and Fermented Foods
- Sphingolipid Metabolism and Signaling
University of Gothenburg
2017-2024
Conventional DC2s are necessary and sufficient for priming T follicular helper cells.
// Patrik Sundström 1 , Louis Szeponik Filip Ahlmanner Malin Sundquist Justin S.B. Wong 2 Elinor Bexe Lindskog 3 Bengt Gustafsson and Marianne Quiding-Järbrink Department of Microbiology Immunology, Sahlgrenska Academy at University Gothenburg, Göteborg, Sweden Pathology, National Hospital, Singapore Microbiology, National, Singapore, Surgery, Correspondence to: Quiding-Järbrink, email: marianne.quiding@microbio.gu.se Keywords: tumor immunity; MAIT cells; cytotoxicity; colon adenocarcinoma;...
The incidence of left-sided colon and rectal cancer in young people are increasing worldwide, but its causes poorly understood. It is not clear if the tumor microenvironment dependent on age onset, little known about composition tumor-infiltrating T cells early-onset colorectal (EOCRC). To address this, we investigated T-cell subsets performed gene expression immune profiling sporadic EOCRC tumors matched average-onset (AOCRC) tumors. Left-sided from 40 cases were analyzed; 20 (<45 years)...
Citrobacter rodentium infection is a murine model for pathogenic intestinal Escherichia coli infection. C. causes an initial decrease in mucus layer thickness, followed by increase during clearance. We aimed to identify the cause of these changes and utilize this naturally occurring stimulus pathogen impact inflammation. identified that mucin production speed transport from Golgi secretory vesicles at apical surface increased concomitantly with thickness. Of cytokines differentially...
Uncontrolled activation of intestinal mononuclear phagocytes [MNPs] drives chronic inflammation in inflammatory bowel disease [IBD]. Triggering receptor expressed on myeloid cells 1 [TREM-1] has been implicated the pathogenesis IBD. However, role TREM-1+ cell subsets driving IBD pathology and link with clinical parameters are not understood. We investigated TREM-1 expression human MNP examined blocking as a potential therapy.TREM-1 gene was analysed mucosa, enriched epithelial lamina propria...
Mucosal-associated invariant T (MAIT) cells are unconventional recognizing microbial metabolites, presented by the MR1 protein. Upon activation, MAIT rapidly secrete cytokines and exert cytotoxic functions, may thus be highly relevant also in tumor immunity. accumulate colon tumors, but contrast to other cell subsets, their presence tumors has been associated with worse patient outcome. Here we investigated if exhaustion contribute reduced anti-tumor immunity cells. Freshly isolated...
Tumor-infiltrating lymphocytes are crucial for anti-tumor immunity. We have previously shown that regulatory T cells (Treg) able to reduce T-cell transendothelial migration in vitro and accumulation of effector intestinal tumors vivo. Treg depletion also resulted increased levels the chemokines CXCL9 CXCL10 specifically tumors. In this study, we investigated mechanisms mediated suppression into APCmin/+ mouse model. By breeding mice with DEREG mice, which harbour a high affinity diphtheria...
Abstract Background Intratumoral regulatory T cells (Treg) in colon cancer are a heterogeneous cell population, with potential impact on patient outcome. Generally, high Treg infiltration has been correlated to worse outcome, but it is still unclear how the composition of different subsets affects relapse and survival. In this study, we used mass flow cytometry characterize tumors corresponding unaffected tissue, followed by correlation clinical parameters Results Using cytometry, defined 13...
Abstract The presence of activated T cells in colorectal cancer tissues is a strong predictor patient survival. Our previous studies have shown that regulatory (Treg) are able to reduce cell transendothelial migration vitro and accumulation effector intestinal tumors vivo the murine APC Min/+ model for microsatellite stable tumors. In this study, we investigated effect Treg depletion on density functions different TCRαβ + TCRγδ populations We used \DEREG mouse model, which harbor diphtheria...
Endothelial cells are key regulators of transendothelial migration and their secretion chemokines expression adhesion molecules facilitates lymphocyte entry into tissues. Previously, we demonstrated that Tregs can reduce T tumors by decreasing endothelial CXCL10 secretion, but the mechanism which this occurs is still not known. In study, aimed to define how decrease tumors. mRNA sequencing intestinal tumor from Treg depleted mice identified neutral sphingomyelinase 2 (nSMase2) as a gene...
Tumor-associated macrophages (TAM) are known to facilitate colorectal cancer (CRC) growth. High macrophage infiltration in thymidine phosphorylase (TYMP) expressing CRC may correspond poor prognosis. The prognostic impact of the expression CD163, a receptor associated with TAM, and TYMP stroma, respectively, tumor tissue is not yet established. aim this study was identify potential associations between CD163 levels relapse-free survival (RFS) patients stage II CRC, if microdissection...
Although mouse models of CRC treatments have demonstrated robust immune activation, it remains unclear to what extent patients' APCs and TILs interact fuel or quench treatment-induced responses. Our ex vivo characterization tumor adjacent colon cell suspensions suggest that contrasting environments in these tissues promoted inversed expression T co-stimulatory CD80, co-inhibitory programmed death (PD)-ligand1 (PD-L1) on intratumoral vs. colonic APCs. While putative tumor-specific...
Γδ T cell infiltration into tumours usually correlates with improved patient outcome, but both tumour-promoting and tumoricidal effects of γδ cells have been documented. Human can be divided functionally distinct subsets based on receptor (TCR) Vδ usage. Still, the contribution these different to tumour immunity remains elusive. Here, we provide a detailed profiling in colon tumours, using mass flow cytometry, mRNA quantification, TCR sequencing. δ chain usage macroscopically unaffected...
B cells interact with T follicular helper (Tfh) in germinal centers (GCs) to generate high-affinity antibodies. Much less is known about how cognate T-B-cell interactions influence Th that enter circulation and peripheral tissues. Therefore, we generated mice lacking MHC-II expressing and, by thoracic duct cannulation, analyzed the efferent lymph at defined intervals post-immunization. Focusing on gut-draining mesenteric nodes (MLNs), show antigen-specific α4β7 + gut-homing effector prior...
Abstract The presence of activated T cells in colorectal cancer (CRC) tissues is a strong predictor patient survival. Our previous research have shown that regulatory (Treg) are able to reduce T-cell transendothelial migration vitro and accumulation effector intestinal tumors vivo murine model for adenomas (APCMin/+). In this study we investigated the effect Treg depletion on cytotoxic potential conventional αβ γδ adenomas. We used APCMin/+ \DEREG mouse model, which harbours high affinity...