A5030770974- Glycosylation and Glycoproteins Research
- Gut microbiota and health
- Helicobacter pylori-related gastroenterology studies
- Probiotics and Fermented Foods
- Carbohydrate Chemistry and Synthesis
- Social and Educational Sciences
- Gastrointestinal motility and disorders
- Cystic Fibrosis Research Advances
- Galectins and Cancer Biology
- Proteoglycans and glycosaminoglycans research
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory Bowel Disease
- Digestive system and related health
- Infant Nutrition and Health
- Ion Transport and Channel Regulation
- International Maritime Law Issues
- Arctic and Russian Policy Studies
- Enterobacteriaceae and Cronobacter Research
- Nutrition and Health in Aging
- International Environmental Law and Policies
- Escherichia coli research studies
- Environmental DNA in Biodiversity Studies
- Mycobacterium research and diagnosis
- Blood properties and coagulation
- Skin Protection and Aging
University of Gothenburg
2016-2025
University of Manchester
2021
Lund University
1995-2021
Sahlgrenska University Hospital
2011-2021
University of Maryland, Baltimore
2021
Hunter Medical Research Institute
2021
University of Virginia
2021
Harvard University
2021
Massachusetts General Hospital
2021
University of Technology Sydney
2021
We normally live in symbiosis with approximately 10(13) bacteria present the colon. Among several mechanisms maintaining bacteria/host balance, there is limited understanding of structure, function, and properties intestinal mucus. now demonstrate that mouse colonic mucus consists two layers extending 150 mum above epithelial cells. Proteomics revealed both these have similar protein composition, large gel-forming mucin Muc2 as major structural component. The inner layer densely packed,...
The normal intestinal microbiota inhabits the colon mucus without triggering an inflammatory response. reason for this and how of is organized have begun to be unraveled. in two layers: inner, stratified layer that firmly adherent epithelial cells approximately 50 μm thick; outer, nonattached usually 100 thick as measured mouse. These layers are around highly glycosylated MUC2 mucin, forming a large, net-like polymer secreted by goblet cells. inner dense does not allow bacteria penetrate,...
<h3>Objective</h3> The inner mucus layer in mouse colon normally separates bacteria from the epithelium. Do humans have a similar and are defects this common denominator for spontaneous colitis mice models ulcerative (UC)? <h3>Methods results</h3> deficient Muc2 mucin, Core 1 <i>O</i>-glycans, Tlr5, interleukin 10 (IL-10) Slc9a3 (Nhe3) together with that dextran sodium sulfate-treated was immunostained Muc2, bacterial localisation analysed. All murine revealed contact Additional analysis of...
Scientific Report18 December 2014Open Access Source Data The composition of the gut microbiota shapes colon mucus barrier Hedvig E Jakobsson Department Medical Biochemistry, University Gothenburg, Sweden Search for more papers by this author Ana M Rodríguez-Piñeiro André Schütte Anna Ermund Preben Boysen Food Safety and Infection Biology, Faculty Veterinary Medicine Biosciences, Norwegian Life Sciences, Oslo, Norway Mats Bemark Microbiology Immunology, Clinical Immunology Transfusion...
Mounting the intestinal barricades Gut microbiota are important for health and well-being, but they need to be kept under control prevented from doing any harm. Birchenough et al. investigated microbial molecules that trigger protective mucus secretion a class of goblet cells in colon. Once detected, an alarm signal is transmitted these via innate immune mediators inflammasome components adjacent cells, generating more repelling invaders. Subsequently, sentinel expelled epithelium their...
Objective The colonic inner mucus layer protects us from pathogens and commensal-induced inflammation, has been shown to be defective in active UC. aim of this study was determine the underlying compositional alterations, their molecular background potential contribution UC pathogenesis. Design In single-centre case–control study, sigmoid colon biopsies were obtained patients with ongoing inflammation (n=36) or remission (n=28), 47 without disease. Mucus samples collected ex vivo, protein...
Background Protection of the large intestine with its enormous amount commensal bacteria is a challenge that became easier to understand when we recently could describe colon has an inner attached mucus layer devoid (Johansson et al. (2008) Proc. Natl. Acad. Sci. USA 105, 15064–15069). The are thus kept at distance from epithelial cells and lack this layer, as in Muc2-null mice, allow contact epithelium. This causes colitis later on cancer, similar human disease Ulcerative Colitis, still...
Colon has been shown to have a two-layered mucus system where the inner layer is devoid of bacteria. However, complete overview mouse gastrointestinal lacking. We now characterize release, thickness, growth over time, adhesive properties, and penetrability fluorescent beads from stomach distal colon. displayed spontaneous release all regions released in response carbachol PGE2, except colon domes Peyer's patches. Stomach had an that was adherent epithelium. In contrast, small intestine...
Mucin-type O-linked oligosaccharides (O-glycans) are primary components of the intestinal mucins that form mucus gel layer overlying gut epithelium. Impaired expression O-glycans has been observed in patients with ulcerative colitis (UC), but its role etiology this disease is unknown. Here, we report mice epithelial cell–specific deficiency core 1–derived O-glycans, predominant developed spontaneous resembled human UC, including massive myeloid infiltrates and crypt abscesses. The manifested...
Cystic fibrosis (CF) is caused by a nonfunctional chloride and bicarbonate ion channel (CF transmembrane regulator [CFTR]), but the link to phenomenon of stagnant mucus not well understood. Mice lacking functional CFTR (CftrΔ508) have no lung phenotype show similar ileal problems humans. We that mucosa in CF adhered epithelium, was denser, less penetrable than wild-type mice. The properties mice were normalized secretion into high concentration sodium buffer (~100 mM). In addition, added...
MUC2, the major colonic mucin, forms large polymers by N-terminal trimerization and C-terminal dimerization. Although assembly process for MUC2 is established, it not known how packed in regulated secretory granulae of goblet cell. When VWD1-D2-D′D3 domains (MUC2-N) were expressed a goblet-like cell line, protein was stored together with full-length MUC2. By mimicking pH calcium conditions pathway we analyzed purified MUC2-N gel filtration, density gradient centrifugation, transmission...
The MUC2 mucin organizes the two mucus layers in colon. This carries a large number of O-glycans that are assumed to be attachment sites for commensal flora found outer layer.Single biopsies from sigmoid colon controls (25) and patients with inactive (13) or active (15) ulcerative colitis (UC) were collected during routine colonoscopy. insoluble was prepared separated by gel electrophoresis, its relative amount estimated, released, glycans analyzed novel sensitive glycomics chromatography /...
The colon mucus layers minimize the contact between luminal flora and epithelial cells, defects in this barrier may lead to colonic inflammation. We now describe an ex vivo method for analysis of properties human mouse small large intestine. Intestinal explants were mounted horizontal perfusion chambers. surface was visualized by adding charcoal particles on apical side, thickness measured using a micropipette. Mucus thickness, adhesion, growth rate recorded 1 h. In colon, ability act as...
Goblet cell diversity An adult human has a gut surface area averaging 30 square meters that is bombarded daily by xenobiotics and microorganisms. Mucus synthesized goblet cells supplies protective barrier coating. Nyström et al. discovered are not all the same along length of gut; rather, they form different functional populations depending on location. Small-intestine mucus laced with antimicrobial peptides permeable to small molecules; downstream, thicker generated excludes bacteria...