A5036876226- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Gut microbiota and health
- Monoclonal and Polyclonal Antibodies Research
- Probiotics and Fermented Foods
- Helicobacter pylori-related gastroenterology studies
- Cystic Fibrosis Research Advances
- Galectins and Cancer Biology
- Microbial Metabolites in Food Biotechnology
- Proteoglycans and glycosaminoglycans research
- Erythrocyte Function and Pathophysiology
- Infant Nutrition and Health
- Digestive system and related health
- Tracheal and airway disorders
- Neonatal Respiratory Health Research
- Pancreatic and Hepatic Oncology Research
- Escherichia coli research studies
- Gastrointestinal motility and disorders
- Ion Transport and Channel Regulation
- Polysaccharides and Plant Cell Walls
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Pancreatic function and diabetes
- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
University of Gothenburg
2016-2025
Vanderbilt University Medical Center
2023
University of Cincinnati Medical Center
2023
University of Liverpool
2021
University of Manchester
2021
ORCID
2021
Institute for Biomedicine
2020
Sahlgrenska University Hospital
1981-2017
University of Oslo
2017
Building Bridges
2016
We normally live in symbiosis with approximately 10(13) bacteria present the colon. Among several mechanisms maintaining bacteria/host balance, there is limited understanding of structure, function, and properties intestinal mucus. now demonstrate that mouse colonic mucus consists two layers extending 150 mum above epithelial cells. Proteomics revealed both these have similar protein composition, large gel-forming mucin Muc2 as major structural component. The inner layer densely packed,...
The normal intestinal microbiota inhabits the colon mucus without triggering an inflammatory response. reason for this and how of is organized have begun to be unraveled. in two layers: inner, stratified layer that firmly adherent epithelial cells approximately 50 μm thick; outer, nonattached usually 100 thick as measured mouse. These layers are around highly glycosylated MUC2 mucin, forming a large, net-like polymer secreted by goblet cells. inner dense does not allow bacteria penetrate,...
<h3>Objective</h3> The inner mucus layer in mouse colon normally separates bacteria from the epithelium. Do humans have a similar and are defects this common denominator for spontaneous colitis mice models ulcerative (UC)? <h3>Methods results</h3> deficient Muc2 mucin, Core 1 <i>O</i>-glycans, Tlr5, interleukin 10 (IL-10) Slc9a3 (Nhe3) together with that dextran sodium sulfate-treated was immunostained Muc2, bacterial localisation analysed. All murine revealed contact Additional analysis of...
Scientific Report18 December 2014Open Access Source Data The composition of the gut microbiota shapes colon mucus barrier Hedvig E Jakobsson Department Medical Biochemistry, University Gothenburg, Sweden Search for more papers by this author Ana M Rodríguez-Piñeiro André Schütte Anna Ermund Preben Boysen Food Safety and Infection Biology, Faculty Veterinary Medicine Biosciences, Norwegian Life Sciences, Oslo, Norway Mats Bemark Microbiology Immunology, Clinical Immunology Transfusion...
Mounting the intestinal barricades Gut microbiota are important for health and well-being, but they need to be kept under control prevented from doing any harm. Birchenough et al. investigated microbial molecules that trigger protective mucus secretion a class of goblet cells in colon. Once detected, an alarm signal is transmitted these via innate immune mediators inflammasome components adjacent cells, generating more repelling invaders. Subsequently, sentinel expelled epithelium their...
Objective The colonic inner mucus layer protects us from pathogens and commensal-induced inflammation, has been shown to be defective in active UC. aim of this study was determine the underlying compositional alterations, their molecular background potential contribution UC pathogenesis. Design In single-centre case–control study, sigmoid colon biopsies were obtained patients with ongoing inflammation (n=36) or remission (n=28), 47 without disease. Mucus samples collected ex vivo, protein...
The colonic mucus layer serves as an important barrier and prevents bacteria from invading the mucosa cause inflammation. regulation of secretion is poorly understood. aim this study was to investigate role in induction colitis. Furthermore, by luminal bacterial products studied. colon anesthetized Muc2 −/− , Muc1 wild-type (wt), germ-free mice exteriorized, mucosal surface visualized, thickness measured with micropipettes. Colitis induced DSS (dextran sodium sulfate, 3%, drinking water),...
Background Protection of the large intestine with its enormous amount commensal bacteria is a challenge that became easier to understand when we recently could describe colon has an inner attached mucus layer devoid (Johansson et al. (2008) Proc. Natl. Acad. Sci. USA 105, 15064–15069). The are thus kept at distance from epithelial cells and lack this layer, as in Muc2-null mice, allow contact epithelium. This causes colitis later on cancer, similar human disease Ulcerative Colitis, still...
Colon has been shown to have a two-layered mucus system where the inner layer is devoid of bacteria. However, complete overview mouse gastrointestinal lacking. We now characterize release, thickness, growth over time, adhesive properties, and penetrability fluorescent beads from stomach distal colon. displayed spontaneous release all regions released in response carbachol PGE2, except colon domes Peyer's patches. Stomach had an that was adherent epithelium. In contrast, small intestine...
Mucin-type O-linked oligosaccharides (O-glycans) are primary components of the intestinal mucins that form mucus gel layer overlying gut epithelium. Impaired expression O-glycans has been observed in patients with ulcerative colitis (UC), but its role etiology this disease is unknown. Here, we report mice epithelial cell–specific deficiency core 1–derived O-glycans, predominant developed spontaneous resembled human UC, including massive myeloid infiltrates and crypt abscesses. The manifested...
Cystic fibrosis (CF) is caused by a nonfunctional chloride and bicarbonate ion channel (CF transmembrane regulator [CFTR]), but the link to phenomenon of stagnant mucus not well understood. Mice lacking functional CFTR (CftrΔ508) have no lung phenotype show similar ileal problems humans. We that mucosa in CF adhered epithelium, was denser, less penetrable than wild-type mice. The properties mice were normalized secretion into high concentration sodium buffer (~100 mM). In addition, added...
MUC2, the major colonic mucin, forms large polymers by N-terminal trimerization and C-terminal dimerization. Although assembly process for MUC2 is established, it not known how packed in regulated secretory granulae of goblet cell. When VWD1-D2-D′D3 domains (MUC2-N) were expressed a goblet-like cell line, protein was stored together with full-length MUC2. By mimicking pH calcium conditions pathway we analyzed purified MUC2-N gel filtration, density gradient centrifugation, transmission...
The MUC2 mucin organizes the two mucus layers in colon. This carries a large number of O-glycans that are assumed to be attachment sites for commensal flora found outer layer.Single biopsies from sigmoid colon controls (25) and patients with inactive (13) or active (15) ulcerative colitis (UC) were collected during routine colonoscopy. insoluble was prepared separated by gel electrophoresis, its relative amount estimated, released, glycans analyzed novel sensitive glycomics chromatography /...
Mucins are proteins that cover and protect epithelial cells characterized by domains rich in proline, threonine, serine heavily glycosylated (PTS or mucin domains). Because of their sequence polymorphism, these cannot be used for evolutionary analysis. Instead, we have made use the von Willebrand D (VWD) SEA domains, typical mucins. A number animal genomes were examined to identify homologues, resulting phylogenetic studies. The frog Xenopus tropicalis stands out because gel-forming mucins...