A5047309158- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Glycosylation and Glycoproteins Research
- Blood transfusion and management
- Hemoglobinopathies and Related Disorders
- Monoclonal and Polyclonal Antibodies Research
- Blood donation and transfusion practices
- Neonatal Health and Biochemistry
- COVID-19 Clinical Research Studies
- Diabetes and associated disorders
- Iron Metabolism and Disorders
- Blood disorders and treatments
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Rabies epidemiology and control
- Blood properties and coagulation
- Carbohydrate Chemistry and Synthesis
- Hematopoietic Stem Cell Transplantation
- Erythropoietin and Anemia Treatment
- Metabolomics and Mass Spectrometry Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Galectins and Cancer Biology
- Hepatitis B Virus Studies
- Trauma, Hemostasis, Coagulopathy, Resuscitation
Columbia University
2016-2025
Columbia University Irving Medical Center
2014-2024
NewYork–Presbyterian Hospital
2012-2023
New York Hospital Queens
2006-2023
University of Colorado Denver
2021
GTx (United States)
2008-2021
Barcelona Provincial Council
2021
University of Denver
2021
University of Virginia
2021
University of Maryland, College Park
2021
Although the CD4 molecule is principal cellular receptor for human immunodeficiency virus (HIV), several CD4-negative cell lines are susceptible to infection with one or more HIV strains. These findings indicate that there alternate modes of viral entry, perhaps involving molecules. Antibodies against galactosyl ceramide (galactocerebroside, GalC) inhibited internalization and in two derived from nervous system: U373-MG SK-N-MC. Furthermore, recombinant surface glycoprotein gp120 bound GalC...
BACKGROUND. Reprogramming of host metabolism supports viral pathogenesis by fueling proliferation, providing, for example, free amino acids and fatty as building blocks.
A plasmid vector, termed pSG5rab.gp, that expresses the rabies virus glycoprotein under control of an SV40 early promoter, was tested in C3H/He mice for induction virus-specific immune responses. Mice immunized intramuscularly with pSG5rab.gp vector developed glycoprotein-specific cytolytic T cells, lymphokine-secreting helper cells TH1 subset, and virus-neutralizing antibodies. vaccinated were fully protected against a subsequent challenge virus.
The SARS-CoV-2 beta coronavirus is the etiological driver of COVID-19 disease, which primarily characterized by shortness breath, persistent dry cough, and fever. Because they transport oxygen, red blood cells (RBCs) may play a role in severity hypoxemia patients. present study combines state-of-the-art metabolomics, proteomics, lipidomics approaches to investigate impact on RBCs from 23 healthy subjects 29 molecularly diagnosed patients had increased levels glycolytic intermediates,...
Over 5 million people around the world have tested positive for beta coronavirus SARS-CoV-2 as of May 29, 2020, a third which are in United States alone. These infections associated with development disease known COVID-19, is characterized by several symptoms, including persistent dry cough, shortness breath, chills, muscle pain, headache, loss taste or smell, and gastrointestinal distress. COVID-19 has been elevated mortality (over 100 thousand already died US alone), mostly due to...
BACKGROUND. Some countries have limited the maximum allowable storage duration for red cells to 5 weeks before transfusion. In US, blood can be stored up 6 weeks, but randomized trials not assessed effects of this final week on clinical outcomes.
BACKGROUNDAlthough convalescent plasma has been widely used to treat severe coronavirus disease 2019 (COVID-19), data from randomized controlled trials that support its efficacy are limited.METHODSWe conducted a randomized, double-blind, trial among adults hospitalized with and critical COVID-19 at 5 sites in New York City (USA) Rio de Janeiro (Brazil). Patients were 2:1 receive single transfusion of either or normal control plasma. The primary outcome was clinical status 28 days following...
N-Linked glycosylation is a common form of protein processing that can profoundly affect expression, structure, and function. generally occurs at the sequon Asn-X-Ser/Thr, where X any amino acid except Pro. To assess impact on core glycosylation, rabies virus glycoprotein variants were generated by site-directed mutagenesis with each 20 acids substituted position an Asn-X-Ser sequon. The efficiency in variant was quantified rabbit reticulocyte lysate cell-free translation system supplemented...
Many eukaryotic proteins are modified by Asn-linked (N-linked) glycosylation. The number and position of oligosaccharides added to a protein the enzyme oligosaccharyltransferase can influence its expression function. N-Linked glycosylation usually occurs at Asn residues in Asn-X-Ser/Thr sequons where X ≠ Pro. However, many not glycosylated or inefficiently. Inefficient one more results production heterogeneous glycoprotein products. These glycoforms may differ from another their level...
The gastrointestinal tract plays a major role in the pathogenesis and pathophysiology of infection by type 1 human immunodeficiency virus (HIV-1). It is potential route for viral entry it site number complications, including both opportunistic infections primary HIV-induced enteropathy. Correspondingly, vivo vitro studies have demonstrated HIV cells lymphoid epithelial origin. HT-29, colonic cell line that infectable with many HIV-1 strains, does not express CD4 protein or mRNA. Recent...
This report demonstrates that galactosyl ceramide (GalCer) or a molecule derived from it may serve as an alternative receptor for human immunodeficiency virus in the nervous system. Recombinant gp120, envelope glycoprotein of type 1, specifically binds to GalCer and its derivatives. specificity was studied by inhibiting binding radioiodinated gp120 with antibodies GalCer, excess unlabeled gp120. Binding activity also removed absorbing liposomes containing GalCer. In addition, studies using...
A plasmid vector expressing the full-length rabies virus glycoprotein (G protein) under control of simian 40 (SV40) promoter has previously been shown to induce upon intramuscular (i.m.) inoculation into mice a specific B- and T-cell-mediated immune response protection against challenge with virulent strain virus. Here we tested two parameters that might affect efficacy this DNA vaccine. First, replaced SV40 original early derived from cytomegalovirus leaving all other intact. Although...
Hyperacute rejection of vascularized, discordant xenografts is generally thought to be initiated when natural antibodies the recipient bind endothelial cells donor organ. While such always occurs, molecular targets have not been elucidated. The aim experiments reported herein was identify molecules on porcine that would recognized by human if a organ were transplanted into (or rhesus). Toward end, it shown major components serum are glycoproteins 115kDa, 125kDa, and 135kDa (gp115/135)....
Objective: To determine the association between a composite measure of serological test results for common infections (Chlamydia pneumoniae, Helicobacter pylori, cytomegalovirus, and herpes simplex virus 1 2) stroke risk in prospective cohort study.Design: Prospective followed up longitudinally median 8 years.Setting: Northern Manhattan Study.Patients: Randomly selected stroke-free participants from multiethnic urban community.Main Outcome Measure: Incident other vascular events.Results: All...
Previous studies of the agglutination erythrocytes by basement membrane glycoprotein laminin have suggested that binds to gangliosides [Kennedy, D.W., Rohrbach, D.H., Martin, G.R., Momoi, T. & Yamada, K.M. (1983) J. Cell. Physiol. 114, 257-262]. Based on following evidence, however, we find specifically sulfatides, not gangliosides. Monogalactosyl purified from sheep with a yield 4.3 mg/kg packed cells, bound high affinity as did authentic bovine brain sulfatide...
Hypoxanthine catabolism in vivo is potentially dangerous as it fuels production of urate and, most importantly, hydrogen peroxide. However, unclear whether accumulation intracellular and supernatant hypoxanthine stored red blood cell units clinically relevant for transfused recipients. Leukoreduced cells from glucose-6-phosphate dehydrogenase-normal or -deficient human volunteers were AS-3 under normoxic, hyperoxic, hypoxic conditions (with oxygen saturation ranging 95%). Red healthy also...
Red blood cell (RBC) transfusion is a life-saving therapeutic tool. However, major complication in recipients the generation of antibodies against non-ABO alloantigens on donor RBCs, potentially resulting hemolysis and renal failure. Long-lived antibody responses typically require CD4(+) T help and, murine models, alloimmunization requires spleen. Yet, it not known how RBC-derived antigens are presented to naive cells We sought answer whether splenic dendritic (DCs) were essential for...