A5016366933- Blood transfusion and management
- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Blood groups and transfusion
- Neonatal Health and Biochemistry
- Analytical chemistry methods development
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Hematological disorders and diagnostics
- Chemical Synthesis and Reactions
- Water Treatment and Disinfection
- Erythropoietin and Anemia Treatment
- Analytical Chemistry and Sensors
- Analytical Chemistry and Chromatography
- Synthesis and Catalytic Reactions
- Chemical Thermodynamics and Molecular Structure
- High Altitude and Hypoxia
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Hormonal and reproductive studies
- Electrolyte and hormonal disorders
- Flavonoids in Medical Research
- Hemoglobin structure and function
- Spaceflight effects on biology
- Polymer Surface Interaction Studies
Hemanext (United States)
2016-2023
Florida Institute of Technology
2022
Baxter (United States)
2007
University of Colorado Boulder
1994-1995
University of Colorado Health
1994
University of Colorado System
1992
Northern Illinois University
1988
William Harvey Hospital
1987
University of California, Los Angeles
1982
University of California, San Diego
1982
Hypoxanthine catabolism in vivo is potentially dangerous as it fuels production of urate and, most importantly, hydrogen peroxide. However, unclear whether accumulation intracellular and supernatant hypoxanthine stored red blood cell units clinically relevant for transfused recipients. Leukoreduced cells from glucose-6-phosphate dehydrogenase-normal or -deficient human volunteers were AS-3 under normoxic, hyperoxic, hypoxic conditions (with oxygen saturation ranging 95%). Red healthy also...
BACKGROUND Being devoid of de novo protein synthesis capacity, red blood cells (RBCs) have evolved to recycle oxidatively damaged proteins via mechanisms that involve methylation dehydrated and deamidated aspartate asparagine residues. Here we hypothesize such are relevant routine storage in the bank. STUDY DESIGN AND METHODS Within framework REDS‐III RBC‐Omics (Recipient Epidemiology Donor Evaluation Study III Red Blood Cell‐Omics) study, packed RBC units (n = 599) were stored under bank...
BACKGROUND Blood transfusion is a lifesaving intervention for millions of recipients worldwide every year. Storing blood makes this possible but also promotes series alterations to the metabolism stored erythrocyte. It unclear whether metabolic storage lesion correlated with clinically relevant outcomes and strategies aimed at improving quality units, such as hypoxic storage, ultimately improve performance in transfused recipient. STUDY DESIGN AND METHODS Twelve healthy donor volunteers were...
State-of-the-art proteomics technologies have recently helped to elucidate the unanticipated complexity of red blood cell metabolism. One recent example is citrate metabolism, which catalyzed by cytosolic isoforms Krebs cycle enzymes that are present and active in mature erythrocytes was determined using quantitative metabolic flux analysis. In previous studies, we reported significant increases glycolytic fluxes cells exposed hypoxia vitro or vivo, an observation relevant transfusion...
Recent research focused on understanding stored red blood cell (RBC) quality has demonstrated high variability in measures of RBC function and health across units. Studies have historically linked this to variations processing, storage method, age. More recently, a large number studies differences donor demographics, iron sufficiency, genetic predisposition the poor storage, particularly through mechanisms accelerated oxidative damage. A study was undertaken evaluate potential additional...
Resuscitation from hemorrhagic shock (HS) by blood transfusion restores oxygen (O2) delivery and provides hemodynamic stability. Current regulations allow red cells (RBCs) to be stored used for up 42 days. During storage, RBCs undergo many structural functional changes. These storage lesions have been associated with adverse events increased mortality after transfusion, increasing the need improved RBC protocols. This study evaluates efficacy of anaerobically resuscitate rats severe HS...
Steroids are an important class of biomolecules studied for their role in metabolism, development, nutrition, and disease. Although highly sensitive GC- LC-MS/MS-based methods have been developed targeted quantitation known steroid metabolites, emerging techniques including ion mobility (IM) shown promise improved analysis capacity to better identify unknowns complex biological samples. Herein, we couple LC-IM-MS/MS with structurally selective reactions targeting hydroxyl carbonyl functional...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAqueous Nitrite Ion Determination by Selective Reduction and Gas Phase Nitric Oxide ChemiluminescenceAndrew J. Dunham, Robert M. Barkley, E. SieversCite this: Anal. Chem. 1995, 67, 1, 220–224Publication Date (Print):January 1995Publication History Published online1 May 2002Published inissue 1 January 1995https://pubs.acs.org/doi/10.1021/ac00097a033https://doi.org/10.1021/ac00097a033research-articleACS PublicationsRequest reuse permissionsArticle...
Objective: Unexpectedly wide distribution (<10 to >90%) of hemoglobin oxygen saturation (sO 2 ) within red cell concentrates (RCCs) has recently been observed. Causes such variability are not yet completely explained whereas the roles and oxidative lesions during storage RCCs known. The objectives present study characterize sO in produced a Swiss blood center investigate influence processing donors’ characteristics. Methods: level was measured 1701 leukocyte-depleted derived...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSulfur-Selective Detector for Liquid Chromatography Based on Sulfur Monoxide-Ozone ChemiluminescenceThomas B. Ryerson, Andrew J. Dunham, Robert M. Barkley, and E. SieversCite this: Anal. Chem. 1994, 66, 18, 2841–2851Publication Date (Print):September 15, 1994Publication History Published online1 May 2002Published inissue 15 September 1994https://pubs.acs.org/doi/10.1021/ac00090a009https://doi.org/10.1021/ac00090a009research-articleACS...
Oxidative stress is a major driving force in the development of storage lesions red cell concentrates (RCCs). Unlike manufactured pharmaceuticals, differences component preparation methods and genetic/physiological status donors result nonuniform biochemical characteristics RCCs. Various donated blood on oxygen saturation (SO2 ) distribution were investigated, model to estimate potential oxidative burden stored RCC at transfusion proposed.The content freshly prepared RCCs (770) was...
Abstract Background Good models of chronic red blood cell (RBC) transfusion can improve care by accurately estimating time‐dependent hemoglobin (Hb) levels and clinically relevant parameters. Study Design Methods A previously derived mathematical model based on overall Hb mass balance (HMB model) with input parameters transfused units, efficiency, RBC lifespan, endogenous Hb, interval was applied to three separate clinical studies spanning six conditions across patient populations...
Anemia is a common symptom of hematological malignancies and red blood cell (RBC) transfusion the primary supportive treatment, with many patients becoming dependent. Hemanext Inc. (Lexington, MA, United States) has developed CE mark certified device to process store RBCs hypoxically - citrate-phosphatedextrose (CPD)/phosphate-adenine-glucose-guanosine-saline-mannitol (PAGGSM) RBCs, leukocytes-reduced (LR), O2/CO2 reduced aim improving RBC quality for transfusion. This interim analysis...
The treatment of (E)-3,4-dimethoxycinnamic acid with ceric ammonium nitrate in trifluoroacetic afforded (E)-1,2-dimethoxy-4-nitro-5-(2-nitroethenyl)benzene 79% yield. unusual ipso substitution the carboxylic moiety by a nitro functional center illustrated new reaction manifold cerium(IV). Six cinnamic acids were examined to ascertain generality transformation. bidentate nitrato structure metal salt is believed account for nitrating ability this system.
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γ-irradiation is used to treat red blood cell (RBC) concentrates (RCCs) transfused immunosuppressed patients. This treatment damages RBCs and increases storage lesions. Several studies have shown the beneficial effect of reducing O2 content during RBC storage. The present research work investigated on RCCs stored under normal hypoxia/hypocapnia conditions.O2 concentration (measured as oxyhaemoglobin fraction, sO2) ABO-matched from whole donations, leukoreduced prepared in phosphate, adenine,...
Abstract A flow injection method is described for the determination of low levels nitrite ion in cell culture solutions. Nitrite-containing solutions are injected into a flowing stream containing phosphoric acid and iodide ion, which reduces to nitric oxide. The solution directed through microporous tube out oxide gas diffuses detection by chemiluminescence. has been applied analysis generated cultured bovine pulmonary artery endothelial cells human neutrophils.
Summary Three cases of premature ovarian failure at a very early age (two patients aged 16 and one 18) are reported. All presented with secondary amenorrhoea. The diagnosis was made after the finding greatly raised serum FSH LH levels confirmed laparoscopy biopsy ovary, when tiny uterus atrophic ovaries seen. Ovarian showed lack primordial follicles. three on hormone replacement therapy.